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The Effects of Equilibration Time and Post-Thawing Temperatures in Cryopreservation of Gaga Chicken Semen

The Effects of Equilibration Time and Post-Thawing Temperatures in Cryopreservation of Gaga Chicken Semen

Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1

1Department of Animal Reproduction and Genetic, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; 2Student of Doctoral program in Animal Science, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; 3Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Brawijaya Jl. Puncak Dieng, Malang 65151, Indonesia

 
*Correspondence | Sri Wahjuningsih, Department of Animal Reproduction and Genetic, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; Email: yuning@ub.ac.id

Figure 1:

Viability of sperm Gaga chicken a. dead sperm b. live sperm.

Figure 2:

Plasma membrane integrity of sperm a. damaged PMI b. intact PMI (swelling in tail of sperm).

Figure 3:

Acrosome Integrity of sperm Gaga chicken using colouring solution of Coomassie Brilliant Blue (CBB) a: integrated acrosome (blue coloured in head sperm) b: unintegrated acrosome (less coloured in head sperm).

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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