Therapeutic Effect of Epigallocatechin-3-Gallate and Quercetin on Renal Stone Formation in Rats
Therapeutic Effect of Epigallocatechin-3-Gallate and Quercetin on Renal Stone Formation in Rats
Youfang Li1, Xu Li2, Shaik Althaf Hussain3, Jayasimha Rayalu Daddam4 and Zhigang Chen5*
ABSTRACT
In the present study, anti-urolithic potentials of epigallocatechin gallate (EGCG), a constituent of green tea polyphenols and quercetin, individually as well as in combination, was evaluated in animal model. In vivo evaluation was carried out on male Sprague-Dawley rats, which were divided into seven groups i.e., Group 1 animals (controls) were fed regular chow and drank water ad libitum; group 2 animals were fed chow containing 3% sodium oxalate with the administration of gentamicin (40 mg/kg) and drank water ad libitum; group 3-7 animals were fed the same diet as group 2 with gentamicin administration and administered with standard drug i.e., allopurinol, EGCG, quercetin and EGCG: quercetin (1:1). All the study animals were sacrificed after 4 weeks of treatment after a 24-hour urine collection, and blood and kidneys were removed for urine and serum biochemical analysis, tissue anti-oxidant, miR-21 as well as morphological examination. We found that administration of Sodium oxalate induced induced oxidative stress, alteration in the serum as well as urinary biochemical alterations as well as spiked expression of miR-21. The alterations were reversed in all the treatment group especially EGCG and EGCG-Quercetin. The reversal potentials of combinational therapy were found to be more effective than the standard drug, Allopurinol. The histopathological analysis also confirmed the same. Although, EGCG has a potent inhibitory effect on urinary stone formation, and the antioxidative action of EGCG is considered to be involved. The combinational activity was enhanced along with quercetin.
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