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Growth Performance and Nutrient Digestibility of Cirrhinus mrigala Fingerlings Fed Phytase Supplemented Sunflower Meal Based Diet

Syed Makhdoom Hussain^1,*, Tasneem Hameed¹, Muhammad Afzal², Arshad Javid³, Nosheen Aslam⁴, Syed Zakir Hussain Shah⁶, Majid Hussain⁵, Muhammad Zubair ul Hassan Arsalan¹ and Muhammad Mudassar Shahzad¹

...stibilityof Cirrhinus mrigala fingerlings fed sunflower meal based diet. The chromic oxide was added as inert marker in the diets. Three replicate groups of 15 fish (Average weight 5.05±0.013 g fish^-1) were fed at the rate of 5% of live wet weigh and feces were collected twice daily. The results of present study showed improved growth performance of Cirrhinus mrigala fingerlings in response to...

Assessment of Heavy Metals and Antioxidant Enzyme in Different Organs of Fish from Farm, Hatchery and Indus River of Pakistan

Zeenat Bano^1,*, Sajid Abdullah¹, Waqas Ahmad², Muhammad Anjum Zia³and Wardah Hassan¹

...ey) of fish, Cirrhina mrigala. The 3 different representative water samples and 60 fish samples (20 fish from each site) were collected from three sites i.e. fish-farm, hatchery and Indus River of Punjab, Pakistan. The results showed differences in physico-chemical parameters of water samples collected from all the sites. The concentrations of Zinc (Zn), copper (Cu) and nickel (Ni) were higher in river water as compared to farm and hatchery.

Comparative Assessment of the Acute Toxicity, Behavior and Catalase Activity in Cirrhina mrigala Exposed to Fe+Ni+Pb+Zn Mixture

Huma Naz^1,*, Sajid Abdullah², Sidra Naz³, Sidra Abbas², Wardah Hassan², Shakeela Perveen², Moazama Batool¹, Laiba Shafique²

...or the fish, Cirrhina mrigala was determined in this research work. The fish were exposed to each of the 19 different concentrations (5-90 mg/L) of Fe+Zn+Pb+Ni mixture to determine 96 h LC₅₀ and lethal concentration. During acute toxicity test, behavioral changes and catalase activity in liver, kidney, gills, brain and muscle of the fish were also studied. The mean 96 h LC₅₀ and lethal value for C. mri

Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan

Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4

Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan

...beo rohita and Cirrhinus mrigala was conducted from January through May, 2015. Water, sediment and fish samples were collected on monthly basis from upstream and downstream of head Trimmu, river Chenab. Throughout the study period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment samples was 71.08±8.32 mg/L. Maxi...

Risk factors for multiple sclerosis in Pakistani population- A crosssectional study

Nasir Raza Zaidi1, Mian Waheed Ahmad1, Mahesh Gautam1, Riffat Mehboob2*

Risk factors for multiple sclerosis in Pakistani population- A crosssectional study

Forecasting percentage contribution of plankton biomass towards increase in fish yield under composite culture conditions

Jhan Zeb, Muhammad Javed

Forecasting percentage contribution of plankton biomass towards increase in fish yield under composite culture conditions

...a, Catla catla, Cirrhina mrigala, Ctenopharyngodon idella and
Hypophthalmichthys molitrix in the ratio of 27:10:10:07:13, respectively. Each triplicate group of fish T1 to T6 were fed
supplementary diets at varying protein level viz., 22, 24, 26, 28, 30 and 32% digestible protein (DP), respectively @
2% of their wet body weight daily. However, control fish (T7) although had an access to plankton; but were devoid of
the availability ...

Effect of Inorganic Cr (Chromium Chloride on Carbohydrate Metabolism in Cirrhinusmrigala (mori)

Farkhanda Asad^1*, Samina Qamer¹, Tayyaba Ali¹, Ammara Behzad¹ and Tahira Yasmin²

...tinized corn in Cirrhinusmrigala fingerlings for 90 days. Using various levels of chromium chloride hexahydrate six test dietsdesignated as; T1(G/0.0, CrCl3.6H2O mg Kg-1), T2(NG/0.0, CrCl3.6H2O mg Kg-1), T3(G/0.2, CrCl3.6H2O mg Kg-1), T4(NG/0.2, CrCl3.6H2O mgKg-1), T5 (G/0.4, CrCl3.6H2O mg Kg-1) and T6(NG/0.4, CrCl3.6H2O mg Kg-1) were prepared. Results showed highest apparent digestibility coefficient (ADC) of nutrientsdry matter, crude lipid and gross energy ...

Inter-Specific Differences in the Sensitivity, Accumulation and Antioxidant Capacities of Three Cyprinids Exposed to Heavy Metals Mixture

Fariha Latif* and Muhammad Javed

...hita and Cirrhina mrigala towards metals mixture (Cd+Cr+Cu+Pb) were determined during present research work. Sensitivity of the three fish species (120-day old) towards metals mixture was measured in term of 96 h LC₅₀and lethal concentration. These acute toxicity tests were performed at constant temperature (30^oC), pH (8) and total hardness (250 mgL^-1). All the three fish species varied significantly in their sensit...

Comparison of Growth, Proximate and Minerals Composition of Commonly Polycultured Fish Species by Replacing Surface Feeder Fish

Muhammad Ahmad, Zohaib Noor*, Karim Johar Khan, Waqar Younas and Ajmal Hussain

... feeder and Cirrhinus mrigala (C. mrigala) bottom feeder, respectively, while, in group 2 only (C. catla) was replaced with (Hypophthalmichthys molitrix) H. molitrix as a surface feeder keeping other species the same as in group 1. At the end of experiment H. molitrix showed considerably high weight gain (P< 0.05) in group 2 as compare to column and bottom feeder fish speci...

Identification of Three Cyprinidae Family Members through Cytochrome Oxidase I

Shahid Sherzada^1,2,4,*, Muhammad Naeem Khan⁴, Masroor Ellahi Babar², Muhammad Idrees³, Abdul Wajid², Muhammad Nauman Sharif³, Muhammad Shahzad Iqbal³, Iram Amin³ and Muhammad Shahid³

...>Labeo rohita, Cirrhinus mrigala, Catla catla)are very suitable fish species for culture in Pakistan. However, the identification and phylogeny of these fish species are of much interest today. DNA barcoding is used as a bio-identification tool for the organism globally. The current study was also conducted for the fast and accurate identification of Indian major carp species. The fishes used in this study were captured from four geographically isolated di...

Lead Induced Oxidative Stress in Cirrhina mrigala

Fariha Latif¹*, Muhammad Javed², Hammad Ahmad Khan² and Khalil-Ur-Rehman³

...and brain of Cirrhina mrigala. The 120-days old fish were exposed to 1/4^th (11.81mgL^-1) and 2/3^rd (31.49mgL^-1) of 96-h LC₅₀of water-borne Pb for three fortnights (42 days) in glass aquaria at pH 7.5, temperature 30°C and total hardness 250mgL^-1. After each fortnight, fish were sampled from each treatment and different organs such as liver, kidney, gills muscles and brain were taken o...

Bioaccumulation of Metals in Fishes’ Scales – A Reliable Non-Lethal Assessor of Food Security

Hafiz Abdullah Shakir^1*, Javed Iqbal Qazi², Abdul Shakoor Chaudhry², Muhammad Irfan³, Muhammad Khan¹, Shaukat Ali⁴and Saima Shahzad Mirza⁵

...ita (rohu) and Cirrhinus mrigala (mori)) from river Ravi during two flow seasons at four sampling locations including upstream Lahore Siphon = A, Shahdera = B, Sunder=C and downstream Balloki headworks = D. All the metal contents in fish scales were highly significantly different (P<0.001) among sampling locations and flow seasons. Location-wise metal accumulation pattern was C > D > B > A. The highest concentrations (µg/g dry weight) of Cd (...

MRI Investigations on Venomous Glands of Brown Bullhead, Ameiurus nebulosus (Lesueur, 1819) (Actinopterygii: Ictaluridae)

Daniel Cocan¹, Vioara Mireşan¹, Florentina Popescu¹, Radu Constantinescu¹, Aurelia Coroian¹, Călin Laţiu¹, Romulus Valeriu Flaviu Turcu³, Alexandru Ştefan Fărcăşanu³ and Cristian Martonos^2,*

Peach Rot Disease Prevalence in the Markets of Federal Capital Territory, Pakistan

Zia-Ur-Rehman¹, Faisal Sohail Fateh^2*, Humayun Saleem¹ and Muhammad Abu Bakar Siddique¹

...na Pul, Burma Chowk, Taramri, NIH Market, Chatha Bakhtawar, I-8, I-10/2, I-10 (Markaz), Sabzi Mandi, I-9. Maximum Mean Disease Index 77.78% was found in the market of Khanna Pul which supplies the fruits to high class, middle class and poor class families. But the study reveals the importance of post-harvest handling of the peach till it reaches to the consumers.
...

Bioaccumulation of Metals in the Organs of Fish inhabiting Ravi River: Serious Threat to Fish and Consumer’s Health

Zarish Yaqoob¹, Saleema Bashir Shams², Gaitee Joshua² and Bibi Nazia Murtaza^2,3*

...0.1 ppm) in Cirrhinus mrigala and Zn (32.9 ppm) in Cyprinus carpio. Maximum accumulation of heavy metals was found in the gills of fish followed by liver, kidneys, muscle and fat cells. Overall, seventeen fish species were recorded in the study areas indicating a decrease in the number of species in Ravi. The results have shown that the water of Ravi is unsafe for aquatic life and irrigation. Metal concentration in the fish meat is higher than th...

Growth and Yield Response of Pea (Pisum sativum L.) Cultivars to Phosphorus Fertilization

Sana Khan¹*, Faiza Aman¹, Muhammad Ismaeel², Zafar Ali², Mehboob Alam¹, Sadeed Iqbal¹ and Taimur Khan³

...and cultivars (Meteor, Samrina Zard, Leena Pak and Pea-2009) to sub plots. Data was collected on growth and yield parameters of randomly selected plants in each treatment and replication. Results showed that phosphorus levels significantly affected all growth parameters. Maximum absolute growth rate (3.31cm day^-1), leaflets plant^-1(102.61), pods plant^-1(16.43), pod length (9.53 cm), root weight (4.67 g), nodules plant^{-1 ...}

Acute Nickel Toxicity Responses of Labeo rohita and Cirrhinus mrigala

Saima Naz¹*, Ahmad Manan Mustafa Chatha², Saba Saeed¹

...beo rohita and Cirrhinus mrigala was determined in this study. During metal stress trials both species were kept at constant temperature (32°C), pH (7) and hardness (250 mg L-1) of water. Physico-chemical parameters of test medium were monitored regularly. The observed mean LC50 and lethal concentrations for Cirrhinus mrigala were 55.85 ± 2.84 and 128.44 ± 9.25 mg L-1, respectively. On the other hand, for L...

Stability of Salmonella typhimurium Bacteriophage to Some Physical and Chemical Factors

Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1

...tious to Salmonella typhimrium ATCC25566 and S.

typhimurium MM11 among the eight bacterial isolates tested belonging to family

enterobacteriacae. The thermal inactivation point of the isolated phage was found to

be 76 ºC. Salmonella bacteriophage (St-1) survived for 7 days at 4, 25, 37, 42 and -

20 ºC. The virus lost its ability to lyse salmonella cells at pH 4, 5, 6, 10, 11 and 12

while it ...

Comparative Analysis of Hematological Parameters of some Farmed and Wild Fish Species

Syed Sikandar Habib^1*, Saira Naz², Sadia Nawaz¹, Iqra Ameer¹, Ameer Khatoon¹, Hameed Ur Rehman³, Sahibzada Muhammad Jawad⁴ and Haider Ali⁵

...>Labeo rohita, Cirrhinus mrigala, Cyprinus carpio, Hypophthalmichthys molitrix, Ctenopharyngodon idella). Fish species selected for the current study is generally widely cultured in Pakistan and worldwide. Fish sampling was done by using different nets (river and farm) and blood sampling was immediately drawn from caudal peduncle by syringe and added in EDTA containing vial and shake well for proper mixing. Vials stored in ice containing boxes and carried ...

Studies on the Wintering Effects on Growth and Survival of Catla Catla Fingerlings in Polyculture System Employing Greenhouse Technology

Kashifa Naghma Waheed*, Tariq Rashid, Sikender Hayat, Muhammad Azeem and Sajid Ali Naqvi

...beo rohita and Cirrhinus mrigala during twelve-week period. The data obtained was subjected to Multivariate analysis of variance (MANOVA) to find out the statistically significant difference (p<0.01) between two treatments for their response to fish growth. The post-hoc test revealed that growth of Major carps was significantly higher with greenhouse as compared to without greenhouse (at p < 0.05). Growth and survival rate of Catla catla were found more ...

Effect of Nano-Se Particles Supplemented Sunflower Meal Based Diets on Mineral Absorption and Carcass Composition of Cirrhinus mrigala Fingerlings

Nisar Ahmad¹, Syed Makhdoom Hussain^2,*, Azhar Rasul², M. Mudassar Shahzad³, Arshad Javid⁴, Hamda Azmat⁵, M. Zubair ul Hassan Arsalan², Sadia Tabassum² and Bilal Ahmad²

...arcass composition of C. mrigala fingerlings fed nano-Se particles supplemented sunflower meal based diets. The experiment was consisted on seven test diets on the basis of supplementation of nano Se graded levels (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg/kg). Chromic oxide was added as an inert marker. Fingerlings were fed at the rate of 5% of their live wet weight. Maximum improvement in mineral absorption (Ca, Na, K, Cu, P and Al) was observed at test diet with 1.5 ...

Genetic Structure Analysis of Freshwater Fish Cirrhinus mrigala by Mitochondrial COI Gene

Shahid Sherzada^1,2*, Muhammad Naeem Khan¹ and Masroor Ellahi Babar²

...analysis of by Cirrhinus mrigala using mitochondrial DNA marker cytochrome oxidase I (COI). Genomic DNA isolated from whole blood was used to amplify and sequence a short region of COI gene in mitochondrial DNA. The identification of sequenced samples was done by NCBI (98-99%) and BOLD (99%) databases. The sequence data analysis represented 15 variable polymorphic sites and 4 haplotypes. Mean haplotypes and nucleotide diversity was 0.42 and 0.0018, respectivel...

Effect of Citric Acid Acidified Moringa oleifera Seed Meal based Diet on Minerals Absorption, Carcass Composition and Hematological Indices of Cirrhinus mrigala Fingerlings

Majid Hussain¹, Syed Makhdoom Hussain², Razia Iqbal³, M. Mudassar Shahzad^4,*, Syed Zakir Hussain Shah³, Afia Muhammad Akram⁴, Nisar Ahmad⁵ and Muhammad Zubair ul Hassan Arsalan²

...cal indices in Cirrhinus mrigala fingerlings. Basal diet was supplemented with 0%, 1%, 2%, 3%, 4% and 5% CA resulting in the formulation of six experimental diets. Ten fingerlings were stocked in tanks in triplicate for each treatment. Feed was given at 5% live wet body weight of fingerlings for 90 days. Results showed that diet acidification with CA significantly (p< 0.05) improved the mineral absorption, carcass composition and hematological indices of C....

Impact of Temperature Variations on Breeding Behavior of Cirrhinus mrigala during Induced Spawning

Saeed Akram Khan¹, Shahid Sherzada¹*, Muhammad Ashraf¹, Durr-e-Shehwar¹, Sonia Iqbal¹, Usman Atique^1,3, Farzana Abbas¹, Sohail Ahmad² and Muhammad Sultan Haider⁴

...ign: justify;">Cirrhinus mrigala naturally breeds in rivers and streams during monsoon season but in ahatchery, it can be induced to spawn by the administration of ovaprim or its analogues. However, the conditions of the induced environment vary from place to place as well as the dose of ovaprim. The present investigation is focused on possible impact of different temperatures on the induced spawning behavior of C. mri...

Management of Spinal Osteochondroma in Young Golden Retriever Dog

Neeranoot Detcharoenyos¹, Nakrob Pattanapon¹, Soontaree Petchdee²*

Effect of Chitosan Edible Coating on the Physico-Chemical and Sensory Characteristics of Stored Mori Fillet (Cirrhinus mrigala)

Hira Jabbin¹, Muhammad Arshad¹*, Naunain Mehmood¹, Wardah Hassan¹, Syed Zakir Hussain Shah² and Farzana Siddique³

A Cross-Sectional Study to Determine the Prevalence, Symptoms and Risk Factors Associated with Low Back Pain and Sciatica in District Nowshera, Pakistan

Sheeza Iqbal* and Asima Azam

Prevalence and Antimicrobial Susceptibility of Bacterial Organisms in Raw Fish of Pond and Retail Market

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1

...me Thaila) and Cirrhinus mrigala (CM; local name Morakhi). The samples were processed for isolation of bacterial organisms using conventional culture method and isolates were tested for antimicrobial sensitivity according to CLSI method. According to results, the prevalence of bacterial organisms was high (p < 0.05) in CC than CM and LR. E.coli were the most common organism in market and pond fish samples with occurrence of 86.6% and 66.6% respectively. Nex...

Stock Assessment of Three Cyprinid Fishes Along the Potamon Zone of Thamirabarani River, Eastern Slope of Western Ghats, South India

Durairaja Ramulu¹*, Jawahar Paulraj², Jayakumar Natarajan¹, Sudhan Chandran¹ Sudhir Kumar Das³, Suresh Eswaran⁴, Santhakumar Rajagopal⁵ and Padmavathy Pandurangan⁶

...ers of Mirgal, Cirrhinus mrigala (Hamiltoon, 1832) (L∞ 89.25 cm, K 0.8y-1), Blackspot barb, Dawkinsia filamentosa (Valenciennes, 1844) (L∞ 22.05 cm, K 0.68y-1) and Roho labeo, Labeo rohita (Hamilton, 1822) (L∞ 47.25 cm, K 0.71y-1) and the mortality parameters of C.mrigala (Z 2.14 y-1, M 1.22 y-1 and F 0.92 y-1), D. filamentosa (Z 2.42 y-1, M 1.54 y-1 and F 0.88 y-1) and L. rohita (Z 2.87 y-1, M 1.42 y-1 and...

The Establishment and Evaluation of an Atherosclerotic Vulnerable Plaque Model Involving New Zealand Rabbits

Wenxiao Jia¹*, Yilinuer Yilihamu¹, Yunling Wang¹, Shuang Ding¹, Dilinuerkezi Aihemaiti¹, Hanjiaerbieke Kukun¹ and Yanhui Ning²

Assessment of Various Biochar Sources as a Dietary Additive in Cirrhinus mrigala Fingerlings to Enhance Mineral Status, Carcass Composition, and Antioxidant Activity

Muhammad Adnan Khalid¹, Syed Makhdoom Hussain¹*, Shafaqat Ali^2,3*, Abdullah Ijaz Hussain⁴, Muhammad Asrar¹, Nisar Ahamd⁵, Majid Hussain⁶ and Muhammad Zubair-ul-Hassan Arsalan⁷

...nt activity of Cirrhinus mrigala. At the conclusion of feeding trial, the protein content was highest (18.51±0.13%) in fingerlings fed with poultry waste biochar and lowest (15.35±0.11%) in fish fed with parthenium biochar. Mineral content was also significantly different in each fish group as compared to control diet. The diet contained Parthenium biochar showed poor results (P<0.05) in terms of minerals except Se (0.65±0.004 mg/kg). A...