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Management of Root-Knot Nematode (Meloidogyne incognita) in Okra (Abelmoschus esculentus L.) Moench) using Trichoderma Species and Poultry Manure

Management of Root-Knot Nematode (Meloidogyne incognita) in Okra (Abelmoschus esculentus L.) Moench) using Trichoderma Species and Poultry Manure

Daniel Offiong Etim1*, Idorenyin Asukwo Udo2, Rosemary Anietie Bassey1, Victoria Barrong Ogar1 and Etim John Umana1

1Department of Botany, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria; 2Department of Crop Science, Faculty of Forestry and wildlife Resources Management, University of Calabar, Calabar, Nigeria.

 
*Correspondence | D.O. Etim, Department of Botany, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria; Email: danieloffiong@yahoo.com

ABSTRACT

The screen house experiment was conducted to evaluate the efficacy of species of Trichoderma in combination with poultry manure in the control of Meloidogyne incognita infecting okra. Trichoderma viride at 2.65 x 107, 2.40 x 107, 1.85 x 107 spores/ml and T. harzianum at 2.40 x107, 1.40 x 107, 1.10 x 107 and 9.0 x 106 spores/ml were applied singly, in combination with poultry manure at 10, 15 and 20 t/ha. The obtained results showed highly susceptibility of okra to M. incognita with Gall index (G.I = 5.00) with no poultry manure amendment or Trichoderma infestation. Increase in poultry manure rates and spore density of Trichoderma applied singly were significantly (P< 0.05) reduced root galling, nematode density in soil per plant and nematode reproduction factor (Rf). The combination of different rates of poultry manure with either species of Trichoderma at various spore densities were significantly (P<0.05) inhibited root galling and nematode population more than single application. The combination of T. viride at 2.65 x 107 spores/ml or T. harzianum 2.40 x 107 spores/ml with 20 t/ha poultry manure produced okra with the least GI (2.00). The combination of Trichoderma species with poultry manure significantly (P<0.05) enhanced growth, dry matter accumulation and fresh pod yield. 

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Pakistan Journal of Nematology

December

Pakistan Journal of Nematology, Vol. 41, Iss. 2, Pages 101-194

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