Johanna Lindahl 1, 2
British Journal of Virology
...ults in more than 60 000 clinical cases of Japanese encephalitis annually, with high case fatality rates. The main vectors preferably feed on livestock and breed in rice fields, and the majority of cases subsequently occur in rural areas. This review lists the risks of emergence of diseases due to the on-going urbanization, with especial focus on vector-borne diseases, particularly Japanese encephalitis. It is concluded, that although the mechanisms required ...
Ye Liu2, Yulong Cong2, Yiming Shao1*
...ines. By highlighting preclinical/clinical applications of Tat and its working mechanisms, this review exhibits the superior potential of Tat as immunoregulator on optimizing immune responses mediated by vaccines. These examples of selected studies will provide researchers some implications and inspirations for using HIV Tat to optimize immune responses of vaccines....
Simon Mwangi Kihu1*, George Chege Gitao1, Lily Caroline Bebora1, Njenga Munene John1, Gidraph Gachunga Wairire2, Ndichu Maingi1, Raphael Githaiga Wahome1, Davis Njuguna Karanja1, Julius Otieno Oyugi3, Ernest Lutomia3
...ck as early as 1995, the clinical disease was observed later in 2006 following dramatic and devastating PPR outbreaks in Turkana County. In this context, an outbreak of PPRV was observed in Tukana County in 2011 and the current study detail the clinical, pathological and laboratory findings of this outbreak. The disease was clinically manifested by the depression, diarrhea, difficult breat...
James Ayukepi Ayukekbong1,2
E-mail | james.ayukekbong@microbio.gu.se
...initiate the appropriate clinical care. Despite the endemicity of dengue in Nigeria, the disease is not a reportable disease and routine diagnosis is neglected. Here I undertake an exhaustive assembly of known records of dengue prevalence in Nigeria and provide a focus for public health intervention....
Nayle Maria Oliveira da Silva1*, Ricardo do Carmo Zanella2
Hu Zenglei1 and Xiufan Liu1, 2*
...with the severity of the clinical disease. Virulent strains of different genotypes induce distinct pathological manifestations in chicken organs, especially in lymphoid tissues. Genotype VIId of NDV, which is currently endemic in many countries of Asian and Middle East, replicates at a significantly higher level, induces more potent innate antiviral and inflammatory response, and causes more severe damages in lymphoid tissues when compared with virulent viruse...
Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
...nd animals seem not show clinical signs. We recommend carrying out further studies, to determine the presence of BTV in goats and wild ruminants, and to identify serotypes present in the country....
Muhammad Arif Zafar1, Murtaz-ul-Hasan1*, Aayesha Riaz1, Asim Shamim2, Muhammad Farooq Iqbal1 and Arfan Yousaf
E-mail | murtazhassan@yahoo.com
...ne animals were selected clinical and sporadic papillomas different body parts. These animals were divided into three groups; Group A animals were operated for partial excision of the lesions, Group B were inoculated with autogenous vaccine alone and Group C were treated with inoculation of autogenous vaccine supplemented with immune-modulators (Levamisole and Vitamin E+Selenium). The animals treated with autogenous vaccine along with immune-modulato...
Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
E-mail | Tomas.Jinnerot@sva.se
...ction can exacerbate the clinical signs. CIRD is highly contagious and can spread rapidly, especially among dogs in close quarters. Fast and accurate diagnostics enables the selection of proper management. Our objective was to develop a sensitive and specific real-time PCR for detection of B. bronchiseptica in samples from dogs with respiratory signs. A genome comparison program was used to select a suitable target DNA sequence. A ...
Claudia Kohl*, Andreas Nitsche, Andreas Kurth
Email: kohlc@rki.de
...e apply this approach to clinical samples we can identify the community of etiological pathogens, without any knowledge on the targets in advance. However, clinical specimens usually comprise an overwhelming amount of host nucleic acids, which by far exceeds the number of pathogen nucleic acids in the sample. Subsequently, it is necessary to either decrease the amount of host nucleic acids or increase the amount of pathogen ...
Muhammad Arif Zafar1*, Arfan Yousaf1, Murtaz-ul-Hasan1, Faisal Ayub Kiayani1, Matee ul Islam2 and Muhammad Farooq Iqbal1
...xt-align: justify;">This clinical report is about the evaluation of complications and survival after using a modified two-layer, hand-sewn, end-to-end anastomosis technique in a calf treated for traumatic umbilical hernia with peritonitis. A cross-bred calf was presented with peritonitis due to traumatic umbilical hernia. The decision to operate the calf was made after confirmation of umbilical hernia resulted in septic shock. Animal was first stabilized with ...
Monireh Ahmadi Bani1*, Mostafa Kamali2, Iman Borji3, Soheyla Aslani3, Francis Fatoye4
...than five minutes is not clinically valuable. This study has evaluated stability with open and close eyes in normal subjects during five minutes standing in twenty healthy subjects. The subjects were asked to stand on a force plate for one and then five minutes, respectively. Their stability parameters were evaluated based on the old and new methods. Paired sample t-test was used for comparison between two methods and different conditions (eyes open and eyes c...
Ali Mohammadi, Mohammad Hossein Ebrahimi
... perform high-resolution clinical trials to evaluate the efficiency of this method....
Ahmed Zein Mahmoud1, Muaz Abdellatif 2, 3*, Luai Shazali1
...ndash;2013 from sick and clinically healthy herds, were subjected to antibodies detection using c-ELISA. Out of examined animals, 83 (62.9%) goats and 70 (33.2%) sheep were detected positive against PPRV antibodies, whereas camels appeared to be seronegative. Based upon the seasonal variations in the antibodies detection, environment appeared to be a significant factor on the level of antibodies in the tested small ruminants population. Taken together, results...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...ive but did not show any clinical signs of PPR. The active PPR virus (PPRV) infection was confirmed by antigen capture ELISA and RT-PCR in both swab and body tissue samples. The molecular characterization revealed clustering of the PPRV within lineage IV with significant substitutions in the nucleoprotein (NP) gene. Genetic variations within NP gene, and possibly in other proteins which are essentially mediating protective immunity, may explain the extreme inf...
Zahir Shah, Shakoor Ahamd, Mian Saeed Sarwar, Murad Ali Khan and Javid Ali
...agnosis was made through clinical sign and physical examination; the area was incised under local anesthesia. After surgical procedure site was closed by applying mattress sutures. Adjunct therapy of broad-spectrum antibiotic was administered intramuscularly for five days as a post-operative measure. Catamnesis revealed that the healing was complete in 14 days with no recurrence and untoward consequences....
Muhammad Ibrahim, Jawaria Ali Khan, Muhammad Sarwar Khan, Wasim Shehzad, Muhammad Avais, Ali Husnain, Naimatullah Khan, Muhammad Kamran Ameen and Abdullah Iqbal
...ents for treatment of subclinical mastitis and to test out how these ingredients can be used for enhancement of milk quality as well as their effects on the haematological values. Total 30 cows were selected for this study kept in different dairy farms in the premises of Lahore. Out of these 30 cows, 24 had subclinical mastitis and 6 were healthy. Cows suffering from subclinical mastitis w...
Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A
...ity of up to 79.12%. The clinical signs observed were prostration, ruffled feathers, diarrhea, anorexia, trembling and mortality that lasted for 6 days. Postmortem findings include ecchymotic hemorrhages in the breast and thigh muscles, mucosa of the junction between the proventriculus and gizzard, severely enlarged and hemorrhagic bursa of Fabricius (BF) and splenomegaly. The bursa of Fabricius was harvested for laboratory investigation using AGID for infecti...
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
...weeks without any direct clinical manifestation of avian influenza. Serum collected from 120 birds was tested for antibodies against H9N2 by using Haemagglutination Inhibition assay. Calculated geometric mean titers of 5.37 revealed the infectivity of the flock with H9N2 Influenza virus. To investigate the presence of virus in the study population, trachea, lung and intestine tissue samples were processed for RNA isolation and subjected to molecular detection ...
KM Fakhrul-Islam, Mohammad Shah Jalal, Sonnet Poddr, Md Nurul Quader, Md Sahidur-Rahman, Avijit Dutta and Shuvo Mazumder
...tive and prospective FMD clinical cases were included considering clinical signs and drugs used for treatment during 13 January to 15 March 2015. A total of 131 cattle was investigated and 16.8% cattle cattle were recorded as FMD. The proportionate prevalence of FMD in different study areas were 4.5-18.2%. The occurrence of FMD was higher in February (23.3%) than in January (13.5%) and March (18.5%). Younger cattle were more...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...s based on case history, clinical sign and clinical examination. Upward fixation of patella was diagnosed in all three animals. After careful palpation of the ligament, medial patellar desmotomy was performed in lateral recumbence, under local anesthesia achieved by 2% lignocaine hydrochloride. The skin was apposed with simple interrupted sutures using silk. Post-operative care was continued for five days.
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
...justify;">
Despite clinical treatments, hepatocellular carcinoma is one of the most common causes of cancer death. We tested whether licofelone, an inhibitor of both cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism, changes the survival rate and induces apoptosis in a hepatoma cell line (H4IIE). We determined in vitro survival rate with MTT assay, the morphological changes with an inverted microscope, apoptosis using flow cy...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...(63/120) with leading subclinical (41.67%, 50/120) form of mastitis on overall milk collected data. Pathogen type was crowned by Staph. aureus with 74.5% (47/63) prevalence, following which were Streptococcus species (17.45%, 11/63), E. coli (3.17%, 2/63), and Bacillus cereus (4.76%, 3/63). Coagulase positive Staph. aureus (41 out of 47), and hemolysin producing Staph. aureus (39 out of 47) primed among Staph. aureu...
Tuli Dey, Sonnet Poddar and Mukti Barua
...erinary college with the clinical sign of complete uterine prolapse which calved one day before. The cow was handled and the prolapsed mass was corrected so carefully that there could be saved the life without any complication. The prolapsed mass of cow was managed with lukewarm water and manual pressure replacement in non-raising condition of hindquarter. Vulvar Retention Suture (modified Bhuners suture) was given for not to recurrence of the condition. Prope...
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
...d in accordance with the clinical laboratory standards and confirmed by PCR. In life birds, only E.coli and Salmonella could be recovered from Cloacal swabs, while in freshly dead birds all four pathogens disseminated in various organs with higher incidence of mixed compared to single infection. Different serotypes of E.coli and Salmonella could be recovered from dead birds however E.coli (O78) and S.enteritidis were recovered mainly from heart and liver. The ...
Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5
... collected from bursa of clinically infected chicks, No morbidity and mortality were observed. During present study, some milder infection with field strain of IBDV almost a week ago recorded were also found in the control group when challenged with IBDV, IBD vaccines in the presence of specific maternally antibodies of IBD could no induce antibodies titer 1500 with live vaccines were protective against the IBDV. Based on the findings of present study, it can ...
Irum, Akram Shah*, Sobia Wahid, Nazma Habib Khan and Qaisar Jamal
...ecies. Based on previous clinical records of dracunculiasis, five villages of Dera Ismail Khan including Kath Garh, Wanda Dost Ali, Chah Mundar Wala, Ketch and Gara Baloch were surveyed for a total of 206 randomly selected households, where the household heads (106 males and 100 females) were interviewed for any previous dracunculiasis lesion scars or active infections among household members. No active cases of dracunculiasis were observed in the present stud...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
... fully protected without clinical signs and virus shedding. Our results revealed that the pCA-Egy-H9 plasmid DNA vaccine could induce complete protection in chickens against H9N2 virus challenge and may propose an alternative strategy to control virus infections in the poultry industry....
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
...e samples collected from clinically sick (60 samples) and apparently healthy broiler birds (30 samples) in a commercial farm, using classical isolation and compared with molecular identification of Mycoplasma infections. A total of 96 tissue samples from trachea, air sacs, lungs and oral swabs along with blood from each bird were collected and initially subjected to isolation of Mycoplasma spp. using brain heart infusion broth supplemented with horse serum. Th...
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...elpful in commercial and clinical application of ADSCs.
...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
... intake could worsen the clinical manifestations in SLE patients, therefore, administration of gluten free diet might be a better strategy for SLE patients. However, further confirmatory studies are required in this regard.
...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
...d with the disorder. The clinical findingsimplicated CTSK and the use of direct sequencing provided a precise molecular diagnosis. Theidentification of the same variant in CTSK as identified in other families from Pakistan suggests that it is common due to a founder effect.
...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
... against NDM-1 producing clinical isolates of Pakistan. A total of five carbapenem resistant bacterial species; E. coli, K. pneumoniae, E. cloacae, A. baumannii and P. aeruginosa, were isolated and identified using VITEK 2 compact system. Carbapenamase and metallo-β-lactamase (MBL) production by these isolates was confirmed by modified Hodge’s test and disc potentiation method, respectively. The blaNDM-1genewas amplif...
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
...evelopment stages of pre-clinical and clinical trials. In this study, we explored the anti-angiogenic properties of three anti-VEGF molecules such as two fusion proteins, VEGF TrapR1R2 and VEGFR1(D1-D3)-Fc, and one DNA aptamer, RNV66, in an in vivo model of human A549 lung cancer. VEGF TrapR1R2 is a commercial anti-angiogenic fusion protein. Our earlier studies demonstrated the anti-angiogenic and anti-proliferative activiti...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...ken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu and 250 cfu from MS and MG respectively in a samples. We have tested pure DNA of other mycoplasmas (5 species from avi...
Tahira Perveen1,*, Shaista Emad1, Saara Ahmad2, Zehra Batool1, Sarwat Yousuf1, Sheeza Sheikh1, Sara Qadeer1 and Saida Haider1
...omparable to that of the clinically used synthetic drug, fluoxetine.
...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4
...ys apart. The birds were clinically observed and swabbed daily. They were killed at 4 and 14 days after single or dual inoculations and were inspected for gross lesions. Samples of the respiratory organs (trachea, lungs, and air sacs) were taken for histological analyses. All the infected subjects showed clinical signs of varying severity. Co-infected groups showed the most obvious clinical
Tania Ahmed Shakoori1,*, Muhammad Saqib Saeed2, Asif Hanif2, Saima Mukhtar3 and Ayesha Ghauri4
...iagnosed on the basis of clinical symptoms and a post bronchodilator values of FEV1/FVC<0.70 on spirometry. Frequent exacerbator’s were defined as patients experiencing 2 or more exacerbation episodes per year, which merited visited to the hospital. Average number of hospital visits for exacerbations per year, smoking history, family history of smoking related chronic lung disease in first degree relatives and presence of comorbidities were recorded f...
Afsheen Masood1*, Rubab Musarrat2, Shama Mazahir3 and Adeela Ashraf4
...ificant implications for clinical professionals, health psychologists, community workers and the future researchers. ...
Arooj Fatima1* and Ashfaq Ahmed2
...ingle blinded randomized clinical trial was conducted at University Physical therapy and Rehabilitation clinic, Raiwind road, Lahore. The study included sample size of 50 subjects diagnosed with rotator cuff tendinopathy. Patients included in study were allocated randomly into; Group-A: This group was treated with routine physiotherapy treatment. Group-B: In this group, subjects were trained with eccentric loading exercises along with routine physiotherapy tre...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
...mel were screened for subclinical mastitis using California mastitis test using convenient sampling technique. Biochemical examination was performed to isolate S. aureus that further were tested Penicillin, Glycopeptide, and Cephalosporin classes of antibiotics for confirmation of MDR isolates using disk diffusion method. MDR were tested in vitro for response against various antibiotics. A questionnaire was filled for estimation of association of...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...fe for consumption as no clinical symptoms were observed in rabbits after giving the maximum dose of 1000ml of color.
...
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...> isolates isolated from clinical samples. In the current study total 794 patients were tested for suspected tuberculosis by fluorescence microscopy and GeneXpert system during the study period (2015-2016) at the Provincial TB Reference Laboratory, Khyber Pakhtunkhwa, Pakistan. Among the 794 patients studied, 170 samples gave positive results. Fifty-two Mycobacterium tuberculosis complex (MTBC) isolates were randomly selected for RAPD-PCR and PCR-restri...
Rahila Nizami1, Muhammad Zahid Latif2*, Intzar Hussain3 and Khalid Rashid4
...o-day bases dealing with clinical directors and managers. This research was conducted to study the leadership styles among the medical professionals. For this purpose, a cross sectional and non-probability convenient sampling was conducted involving 59 medical professionals of Azra Naheed Medical College, Lahore. The assessment of leadership-style (T-P leadership rating scale) was applied. The age of subjects was 37.42±11.91 years; 29(49.15%) were males...
Nukhbat-Ullah Awan1*, Muhammad Sohail2, Zeeshan Sarwar3, Muhammad Mustahsen Bashir4, Muhammad Abbas Khokhar5 and Asad Aizaz Chatha6
Mian Sajjad Ahmad1, Somia Iqtadar1, Sami Ullah Mumtaz1*, Zafar Niaz1, Iqra Waheed2 and Sajid Abaidullah1
...o hours was sent to KEMU clinical lab. A positive result of the patient was labeled as IGT. The patients mean age was 46.3±13.4 years. Out of 160 patients, 78 (48.8%) were males and 82 (51.2%) females.116 (72.5%) patients were obese grade 1 and 44 (27.5%) patients were obese grade 2. The mean BMI (Normal BMI=18.5-22.9kg/m2) of the patients came out to be 29.1±13.6 kg/m2.There were 116 (72.5%) patients of obese-1 and 44 (27.5%) patients of obese 2...
Halima Shoukat1*, Hafiz Sheraz Arshad2, Faiza Sharif1, Arooj Fatima1 and Filza Shoukat1
...eat muscle tightness. In clinical settings, different stretch holding times are used to treat hamstring tightness. The purpose of the study was to determine effects of different static stretch holding-times on hamstring muscles flexibility and their impacts on improving range of motion (ROM) of knee extension in adults. This experimental work was conducted on students of University of Lahore from December 2013 to March 2014. The study comprised of 60 subjects ...
Muhammad Kashif Maan1, Muhammad Arif Khan1, Shehla Gul Bokhari1, Muhammad Ijaz2, Hamid Akbar1, Sajid Umar 3 and Muhammad Luqman Sohail4,*
...esthesia, isoflurane was clinically compared in healthy female rabbits (n=16) undergoing ovariohysterectomy. One group of 8 rabbits was anesthetized with combination of xylazine (3mg/kg) and ketamine (10mg/kg, I/V), while the second group (n=8) was pre-anesthetized with xylazine, followed by treatment with isoflurane (4%) with oxygen flow rate of 1L/min. Rabbits administered with xylazine-ketamine anesthesia showed significant increase (P<0.05) in serum ala...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
...pre-S2 region of the two clinically diagnosed Surface Antigen of Hepatitis B Virus (HBsAg). Pre-S2 region nucleotide sequence of the sample MR02 was found completely homologous with the Pre-S2 region of already reported subtype “adr4”. This sample showed 1.2 to 20% variation with the other reported sequences. Nucleotide sequence of the Pre-S2 region of the second sample MR03 does not show 100% homology with any of the reported sequences. It differs...
Wazha Mugabe1,3,Gaolebale Segolame Mpapho1, John Kamau1, Wameotsile Mahabile2, Shalaulani James Nsoso1, Kealeboga Dipheko1 and Assar Ali Shah3,*
...t diagnosis was based on clinical observations (68) with the low utility of California Mastitis Test (CMT) (3%). Milk production was at subsistence scale and a secondary product from meat type breeds. With a commercial mindset, improved goat management and cooperative pooling and marketing of milk the current farming setup of using meat breed for milk could prove beneficial to both the meat and dairy sectors.
...
Anza Zahid*, Muhammad Ali Ayub, Mujtaba Saeed, Nasir Pasha, Abdul Jabbar Dar, Huzaifa Javed, Aimen Shakeel, Amna Nasir, Ana Akbar, Zainab Tarrar and Ayesha Humayun
...positively impact on the clinical outcome of the ailments and their therapies. ...
Aitezaz Ahsan1*, Muhammad Usman1, Ihsan Ullah2, Aamer Bin Zahur3 and Adnan Rasheed Malik4
...e East and Asia. Besides clinical signs and symptoms of PPR various diagnostic assays, both serological and molecular, have been developed and are applied for estimating the current status of the disease. This review article focuses on the development of different molecular diagnostic assays from conventional reverse transcriptase polymerase chain reaction to real time reverse transcriptase polymerase chain reaction and reverse transcriptase loop mediated isot...
Abid Hussain1,*, Mansur-ud-Din Ahmad2, Muhammad Hussan Mushtaq2, Mamoona Chaudhry2, Muhammad Sarwar Khan2, Michael Reichel3, Tanveer Hussain4, Amjad Khan2, MuhammadNisar2 and Imtiaz Ahmad Khan5
...tigate the prevalence of clinical and subclinical mastitis in dairy buffaloes. The present study was conducted in two districts Lahore and Bhimber Azad Kashmir Pakistan. Total sample size for this survey was consisted of 1,036 lactating buffaloes. The screening of lactating buffaloes was performed with the California Mastitis Test. The overall prevalence of mastitis was recorded at 49%. Among mastitis cases the prevalence of...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...n. Data were obtained by clinical testing of 1,440 ß thalassaemia major patients visiting for blood transfusions at regular intervals. The confirmatory screening for HBV and HCV was performed through Chemiluminescent Immunoassay (CLIA). Of the total 1,440 patients studied, 930 (64.6%) were males and 510 (35.4%) were females. The patient age ranged from 1 to 30 years were with mean age of 7.9±4.5 years. Among 1,440 patients, 44 patients were positi...
Riyadh S. Aljumaah*, Mohammed A. Alshaikh, Abdulrahman Jarelnabi, Mutassim M. Abdelrahman and Mansour F. Hussein
.... All camels tested were clinically normal. Out of 253 camels of either sex, 117 (21.99%) were found to be serologically positive to specific anti-N.caninum antibodies. Logistic regression analysis and estimation of odds ratio revealed significant association of the sex and location of the camels with N. caninum seropositivity. On the other hand, neither the breed nor age of the camels was significantly associated with seropositivity.Studies are ...
Sherin Reda Rouby
...oxviruses from different clinical samples and differentiate capripoxviruses without the need of gene sequencing. A total of 16 lumpy skin disease clinical samples (skin nodules, scabs, buffy coat, lymph aspirate and engorged ticks) and three sheep pox biopsy skin samples were subjected to DNA extraction followed by 30 kDa RNA polymerase subunit gene-based polymerase chain reaction (PCR) (RPO30) together with tissue cult...
Hasina Baloch1, Rahmatullah Rind2,*, Muhammad Rafique Rind3, Viram Kumar4, Nazia Baloch5 and Rajesh K. Oad6
... factors associated with clinical and subclinical mastitis in buffaloes. Significantly higher prevalence (49. 07%) of clinical mastitis was observed in buffaloes with average age of 6 to 9 years whereas those of > 9 years of age showed higher (33.33%) but non-significant prevalence of subclinical mastitis. However, significant impact of the numbe...
Xi-wen Chen1,2, Qian Wang1,3, Miao Yin1, Zhong-hui Pu1,2, Ai-wei Guo3, Lian Li 1,3, Wen-tao Luo1,3 and Xiong-qing Wang1,*
...V, PCV-2, PPV or PRV. In clinical samples (n=5) RT-PCR and RT-LAMP both identified the same 2 PRRSV infected samples. Thus, the novel RT-LAMP assay described here is a rapid, simple, sensitive, specific test for PRRSV that can potentially be applied in clinical settings.
...
Kaleem Ullah1, Zafar Niaz2, Sami Ullah Mumtaz3, Sajid Abaidullah4*
...r positive signs for the clinical, ECG, x-ray chest PA view and pulmonary function tests. Additionally, they were subjected to echocardiography and left ventricular diastolic dysfunctions were noted.
Results: In our study, mean age of the patients was 60.7±6.2 years and mean FEV1/FVC ratio was 56.9±14.3%. Similarly mean ejection fraction of the patients turned out to be 60.6±4.6. The mean peak mitral filling velocity of ...
Maaz Ahmad1*, Mussab Ahmad2 and Tehreem Munir3
...centered, and randomized clinical trial through systematic random sampling was conducted in Lahore urban community.After initial diagnostic investigations in 200 patients diagnosed as cases of FD fulfilling Rome III criteria were recruited after taking written consent and randomly assigned to one of 2 treatment groups (CP extract group or placebo) in equal number. A 7 days medication free period was observed before the start of trial. Each patient received the...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
...y 2017. A total of 12126 clinical samples were collected from patients presenting to Jinnah Hospital, Lahore. Every sample was processed for bacterial culture. Bacterial identification was performed according to standard guidelines. Every gram-negative isolate was further processed for antimicrobial susceptibility testing by modified Kirby Baur disc diffusion method. Zone sizes were interpreted according to CLSI 2016 guidelines. Next day every carbapenem-resis...
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
...are associated with many clinically important parameters of good prognosis. The objective of this study was to detect the frequency of TEL-AML1 fusion oncogene in ALL and its association with already established prognostic factors such as age, WBC count and FAB subtype.
Materials and Methods: Sixty-six patients with newly diagnosed ALL were studied and patients on chemotherapy and T-ALL were excluded. The data was analyzed by SPSS20 for prog...
Zartasha Anjum1*, Rakhshindah Bajwa2 and Munazza Hassan3
Shumaila Irum1, Muhammad Zafar Iqbal*2 and Fatima Naumeri3
... medical students in pre-clinical years about educational environment in a public sector medical college of Pakistan and to compare the DREEM scores with previous published scores.
Material and Methods: The DREEM questionnaire was conducted on undergraduate medical students (n=300) of Sheikh Zayed Medical College, Rahim Yar Khan, during the month of June 2015.
Results: Two eighty-six (n=286) of the 300 students (95.3...
Muhammad Sarfraz*, Shahida Hasnain
...ta-lactamases (AmpC) has clinical significance due to developing resistance of clinical pathogens against several beta-lactam antibiotics. This study was conducted on 11,725 specimens collected from hospitalized and non-hospitalized patients from July 2013 to June 2016. API 20E system has been used to identify K. pneumoniae and E. coli; preliminary screening for AmpC beta-lactamase production was done by cefoxi...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...ively. The MBL producing clinical isolates were further subjected to PCR for the existence of blaIMP and blaVIM genes. The carbapenem resistant A. baumannii (n=32), P.aeruginosa (n=26), K. pneumoniae (n=19), E. coli (n=16), C. ferundi (n=04), P. vulgaris (n=02) and E. cloacae (n=01), were isolated from clinical samples of hospitalized patien...
Shabana*, Saleem Ullah Shahid and Shahida Hasnain
... BMI. The progression of clinical forms of obesity can be assessed by the strong predicting indices, including weight, BMI, low HDL and high TC.
...
Qiang Li1,2, Guo Qiao2, Li Wang1, Jipeng Zhang1, Ruijun Li1, Ping Ni1, Yi Guo1 and Shigen Ye1,*
...cember in 2014. The main clinical signs included fin ulceration, skin darkness, hepatohemia and intestinal hydrops. Totally, 104 diseased live fish were collected and 70 strains isolated from naturally diseased T. rubripes. Most isolates were obtained in May, September and December. The isolates were identified through 16S rRNA gene sequence analysis and Vibrio spp.-specific PCR amplification, followed by pathogenicity determination. Results show...
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
... corneal stroma. Careful clinical evaluation, early diagnosis, genotyping, genetic counseling and proper treatment (corneal grafting) are necessary for the restoration of optimal vision and should be done to avoid the severe outcomes caused by MCD.
...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2
...f Punjab on the basis of clinical biomarkers. Blood and mosquito samples were collected. Blood samples were processed through complement fixation test and RT-PCR, while mosquito samples were processed through RT-PCR. Overall prevalence of bovine ephemeral fever (BEF) in indigenous breeds of bovine was 63.33%, lower, than exotic breeds 64.66% which were found to be more prone to disease. In the case of buffalo breeds, prevalence was more in Nili Ravi buffalo, <...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
...f 30 dogs, comprising 10 clinically healthy and 20 dogs with leishmaniasis were enrolled in this study. The diagnosis of canine visceral leishmaniasis was performed by the immune fluorescence antibody test with antibody titter ≥1:128 and lymph node smear examination. In blood samples of dogs with visceral leishmaniasis, levels of malondialdehyde, total antioxidant capacity, glutathione, nitric oxide, myeloperoxidase, adenosine deaminase and cytokines (TNF-a...
Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*
...ates the incidence of subclinical mastitis (SCM), associated risk factors, involvement and antimicrobial susceptibility of Staphylococcus aureus in the development of SCM in District Faisalabad of Pakistan. For this purpose, a total of 384 goat milk samples were screened for SCM through surf field mastitis test (SFMT) and mastitis-positives cases were further investigated for isolation of S. aureus using standard procedures. Coagulase gene was PC...
Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1
Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
...valence of mastitis (sub-clinical and clinical) before and after parturition and
bacteriological cure rate. Quarter and animal wise prevalence of both clinical and
sub-clinical mastitis after systemic dry cow therapy with lispiracin™ group (G1) was
lower than control group (G2). This was evaluated through Surf Field Mastitis Te...
bacteriological cure rate. Quarter and animal wise prevalence of both clinical and
sub-clinical mastitis after systemic dry cow therapy with lispiracin™ group (G1) was
lower than control group (G2). This was evaluated through Surf Field Mastitis Te...
Nabila Roohi, Mehjabeen, Samina Ashraf*
Effects of cigarette smoking on serum proteins profile in male active and passive smokers
... kits by using automated clinical chemistry analyzer. Serum total proteins
have shown a significant decrease in smokers in comparison with controls. A
significant depression in serum albumin was observed in smokers due to increased
excretion of albumin in bound state with the chemicals, whereas, total globulins,
gamma globulins and non-gamma globulin serum proteins have shown significant
increase in smokers. Our findings prov...
have shown a significant decrease in smokers in comparison with controls. A
significant depression in serum albumin was observed in smokers due to increased
excretion of albumin in bound state with the chemicals, whereas, total globulins,
gamma globulins and non-gamma globulin serum proteins have shown significant
increase in smokers. Our findings prov...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
... was made based upon the clinical and histopathological examination of the tumor sample. In all cases, tumor resection was performed followed by cauterization of the remaining surface with silver nitrate. No postoperative complications or local recurrence were reported by owner of each case.
... 
