Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...essed. Sperm progressive motility, plasma membrane integrity, acrosomal integrity, viability and chromatin integrity was higher (P<0.05) in extender containing melatonin 0.1 mM and 0.5 mM compared to control. In conclusion, the addition of melatonin 0.1-0.5 mM to extender improved the post-thaw quality of buffalo bull semen....
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...awed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response on the post-thawed s...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
... Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane int...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...rs such as sperm volume, motility, morphology, viability, and concentration. In conclusion, gradation of electrical stimulation into three stages (our modified Method II) could help to ease the collection of semen samples from bulls with minimum discomfort signs. Furthermore, the modified method is also recommended to use for other animals, in particular, the wild animals.
...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...nd tested for individual motility, progressive motility, plasma membrane integrity, acrosomal and DNA integrity, seminal pH and dead sperm percentage in fresh and thawed semen. Data were subjected to one way analysis of variance. High sperm individual motility (85.12 ± 0.51%), progressive motility (20.25 ± 0.47%), plasma membrane integrity ...
Maryum Fakhar, Iqra Jabbar, Anjum Nasim Sabri*
...assessing the adherence, motility, hydrophobicity, aggregation and biofilm formation of two salt tolerant strains [Bacillus subtilis and Halomonas aquamarina (1)] in the presence of nickel. Two concentrations of nickel were used i.e., 500μg/ml and 1000μg/ml for subsequent tests. The results revealed that at low/ sub-inhibitory concentration of nickel (500 μg/ml), motility is inhibited; adhesion, hydrophobicity, aggr...
Filiz Kutluyer*
... mM] for assessing sperm motility and its duration, fertility and hatching rate of eggs. The results from the present study indicated that addition of L-tryptophan increased the motility rate and duration in O. mykiss, S. rizeensis and S. coruhensis compared to control group. Highest sperm motility rate and its duration, and fertility and hatching rate of eggs of O....
Yang Yang1,2, Yun Liu1, Mingxu Zhou1 and Guoqiang Zhu1,*
...pression, as measured by motility assays, bacterial gene expression, and host responses to infection. The QS systems and flagellin were coordinately regulated, as deleting fliC caused decreased QS-II activity.
...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...resh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination, pH was determined by digital pH meter. ...
Siddra Tayyab Akhtar, Anjum Nasim Sabri
Twitching, swimming, swarming in biofilm forming strains in response to chemical and physical factors
...control
bacterial motility. Present study determined swimming, swarming, and twitching
motilities in response to different physiochemical factors. Twenty-two
morphologically different bacterial strains were collected from a drain of sewage
water located in the vicinity of Jinnah hospital Lahore, Pakistan. Six biofilm forming
bacterial strains were selected on the basis of their motility and...
bacterial motility. Present study determined swimming, swarming, and twitching
motilities in response to different physiochemical factors. Twenty-two
morphologically different bacterial strains were collected from a drain of sewage
water located in the vicinity of Jinnah hospital Lahore, Pakistan. Six biofilm forming
bacterial strains were selected on the basis of their motility and...
Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2
...ty was analyzed by adult motility assay (AMA). Levamisole was used as positive control to compare the efficacy of CEE of both plants. Adult worms were treated with different concentrations i.e., 2, 4, 6 and 8 mg/ml of ethanolic extract and motility of the worms was examined after every two to eight hours. Both A. lebbeck and C. sinensis exhibited 88% and 95% mortality at 6 & 8 mg/ml after 8 hrs of tr...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
... Sperm concentration and motility were significantly (P<0.05) high in Jersey bull while live sperm percentage was significantly (P<0.05) high in Jersey bulls and sperm abnormality was significantly (P<0.05) high in Sahiwal and Friesian-Sahiwal cross. In addition, semen quality was improved in all the breeds after supplementation of vitamin C. There was no significant effect of BCS on the sperm quality of bulls. The results give important information r...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...testing indices of sperm motility, mitochondrial activity, membrane integrity and arosome integrity. The results illustrated that compared with controls, above-mentioned indices of sperm were significantly improved under concentrations of Vitamin C (400 mg/L) and Vitamin E (600 mg/L) (P < 0.05). Moreover, physiological indices of donkey semen were significantly higher than the control groups (P < 0.05) when Vitamin C (200 mg/L) and Vitamin ...
Naimat Ullah1, Sarzamin Khan2, Muhammad Mobashar3, Shahbaz Javaid4*, Tarique Tonio1 and Asif Mahmood5
...ty parameters i.e. sperm motility and progressive sperm motility (%), sperm concentration (million/ml), live to dead ratio of sperm, amplitude of lateral head displacement of sperm (µm) and straightness of sperm (%) were measured at interval of 15 days. The results showed that dietary supplementation of fat didn’t influence dry matter and water intakes as well as weight gain significantly (P>0.05) among differ...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
... assessment. Progressive motility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-cysteine supplemented groups in comparison with 0.5 mM or 0 mM. The number of supra-vital hypo-osmotic swelling test positive spermatozoa at 1.5 mM and 1.0 mM was greater in comparison with lower concentration of 0.5 or 0 mM L-cysteine. Furthermore, significant improvement for acrosomal integrity was recorded in semen samples treated with 1.5 mM L-cy...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
...mg/kg dosage), decreased motility (for 110 mg/kg dosage) and increased sperm malformation (for all of the treated groups) were also confirmed (p<0.05). Sperm malformation showed high sensitivity to PFOS exposure. A sperm malformation percentage, varying from 11.00% to 51.20%, was confirmed for the treated groups. As to various sperm malformation types, curved body type constituted the most proportion. The treated groups showed a curved body malformat...
Amit Sharma1* and Pankaj Sood2
...tozoa leading to reduced motility, viability, impaired transport and fertility. Caprine sperms are extremely sensitive to cryopreservation compared to other species which is mainly attributed to the compositional variation of the sperm plasma membrane. Different factors affect affects the semen cryopreservation viz. species, breed, semen collection technique, collection season, extender composition, type of cryoprotectant used, cooling rate, equilibration tim...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...hat showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buserelin acetate (Buserelin® aniMedica, Poland). After equilibration, the straws were frozen in liquid nitrogen vapour using a computer controlled automatic freezer. After one day, straws were thawed in a water bath at 38ºC for 20 sec and then were examined...
Paul Oludare Adetunji* and Safiriyu Idowu Ola
...ion, sperm output, sperm motility and sperm abnormalities) from 26-52 weeks of age and sexual ornaments’ morphometry (Comb Length-CL, Comb Height-CH, Wattle Length-WL, Wattle Height-WH and Inter Pubic Distance-IPD) from 16-24 weeks of age. Data on onset of puberty and semen quality parameters were subjected to Analysis of Covariance (ANCOVA) with body weight at puberty as a covariate while morphometry measurements were subjected to Spearman correlation a...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
... semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at ...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...on per mL conc., >60% motility) and cryopreserved using standard procedures. Percent sperm motility, liveability, plasmalemma integrity and viability were increased in extender (P<0.05) having 20.0 ng/mL of arachidic acid compared to 25.0 ng/mL, 30.0 ng/mL and control. However, sperm chromatin integrity was equally improved in experimental extenders having arachidic acid compared to control. Sperm abnormalities were re...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2, Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5, Hamayun Khan1 and Shabana Naz6*
...ighest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility<...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...men concentration, sperm motility and useable spermatozoa/ejaculate before 32 wk of age (p>0.05). Feed quantity was correlated positively with sperm concentration and sperm motility. At 50 wk of age, higher sperm motility was observed in males fed with 135.5and 125.5 g/d diet than males fed with 115.5 g/d diet (p<0.05). While feed quantity was correlated positively with semen volume,...
Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2
...d to determine the sperm motility. PNA-FITC staining was used to examine the acrosome integrity under a fluorescence microscope. Total oxidant status (TOS) and total antioxidant status (TAS) were measured using ELISA. The methionine group showed an increase in the post-thaw motility (53.08 ±3.6%) and viability (47.5 ±2.9) percentages compared to the control group (46.68 ±2.9; 40 &plus...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...sed and found that total motility, progressive motility, membrane functionality, acrosomal integrity, and viability percentage were significantly higher (P < 0.05) in the diluent groups treated with 7.5 mmol of L-cysteine or 4.8 mmol of vitamin E. Correspondingly the DNA integrity and mitochondrial membrane potential percentage were found significantly higher (P < 0.05) in diluent groups treated with 7.5 ...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
...xtender on mortality and motility of spermatozoa of Limousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 % SFE and G4 = 8 % SFE. Each of the treatments was repeated thr...
Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*
...ture, variation in rumen motility, mucous membrane, salivation, edema, dullness, depression, nasal discharge, respiratory distress and recumbence or death. A significant change in complete blood count (CBC) including packed cell volume (PCV), hemoglobin (Hgb) concentration, red blood cells (RBCs) count, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) platelets count, mean platelet volume (MPV),...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...amples were assessed for motility, oxidative stress, sperm membrane integrity, morphology, and acrosome integrity. Four stallions were nominated for AI in the current study. The best result from different concentrations of cysteine and ascorbic acid were used for AI. Thirty mares were divided into three groups of 10 mares each and were then inseminated using the frozen semen with the following extenders: HF-20 extender (0) as control, 0.5 cysteine, and 0.5 asc...
Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5
...ed by flagellum-mediated motility. Type III protein secretion systems of several gram-negative bacterial pathogens use flagella to invade foreign surfaces, host tissues and substrates. Flagellar biosynthesis and function in Salmonella typhimurium isregulated by >50 genes. Bioinformatics analysis of flagellar assembly in S. typhimurium identified several conserved structural elements. In this study, FliI a flagellar protein required for flagell...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...d were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed at the comparatively lower concentration (0.1-1.5%) on the post-thawed quality indicators. On the other hand, the hight GT concentration (1.5%) significantly decreased the quality indicators of spermatozoa. Green tea extract enhanced the spermatozoa survivability, integrity of acrosome and...
Nour El-Hoda Khayrat Hammad1*, Yousef Y. El-Seady3, Azza E. Hassan1, Sara T. Elazab2, Magdy S. Amer2
...ermatozoa (viability and motility) which accompanied by some histopathological damage in testes and epididymis. On a molecular basis, mRNA expression of CYP17A1 and LHR genes were significantly reduced in testicular tissues of FCZ group while aromatase gene was elevated. The administration of LIN oil after FCZ treatment markedly improved the aforementioned alterations caused by FCZ. So linseed oil is capable to ameliorate the fluconazole induced fertility diso...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...valuated were percentage motility, plasma membrane integrity (HOST assay), acrosomal integrity (NAR), viability (Live/Dead), DNA integrity (Acridine orange assay) and oxidative stress (TBARS assay). Five straws from each Gallic acid group were thawed individually in water bath at 37°C for 30 seconds. The addition of 15 µM GA to semen extender improved marginally the buffalo bull spermatozoa motility, viability and ...
Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1
...meters of sperm (volume, motility, concentration, survival) of rams bred in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 109 ml-1 ): 2.66 ± 0...
Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1
...meters of sperm (volume, motility, concentration, survival) of rams bred in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 109 ml-1 ): 2.66 ± 0...
Luke Chukwudi Ali*, Nnanna Ephraim Ikeh, Bright Chigozie Amaefule, Amarachi Linda Obinna, Ndubuisi Samuel Machebe
...hest value (8.67). Sperm motility, sperm concentration, live and normal sperms in decreased with an increase in dietary DCSM whereas non-motile, dead and abnormal sperms increased with an increase in dietary DCSM. Testicular morphometric measurements and histomorphological evaluations were similar in all treatments (P>0.05). This investigation depicts that DCSM inclusion above 15% affected semen quality negatively even though results on testicular morphomet...
Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*
... F18ab△hcp1, bacterial motility, adherence and invasion towards the intestinal porcine enterocyte cells (IPEC-J2) were reduced significantly, and the cytotoxicity decreased. The results of RT-PCR showed that in F18ab△hcp1, the flagella gene flic and type I fimbriae fimA were significantly down-regulated. In conclusion, hcp1 gene of F18 STEC is related to flagella expression, bacterial adherence, invasion, and secretion of Stx2e toxin. T6SS hcp1 is proved t...
Gamal Shawki1, Tawfek Mohamed Barakat1, Attia Mohamed Samy2, Ahmed Alhamed AL-Mejren2, Ayman Mesalam1*
...m count, livability, and motility during June and July. Similarly, the length, width, height and volume of testes as well as the width of total scrotum were higher (P < 0.05) during August, July and September. Moreover, the number of mounts per ejaculation, reaction time, time to erection, ejaculation times and time to ejaculation were significantly lower during summer and spring seasons (P < 0.05). A positive correlation (P < 0.05) between sperm para...
Emtenan M. Hanafi1*, Fouad M. Tawfeek2, Walid S. El Nattat1, Moetazza M. Alshafei3, Seham S. Kassem3, Kawkab A. Ahmed4, Enas N. Danial5, Hagar Elbakry3, Hoda S. El-Sayed2
...owed low sperm count and motility. Histopathological examination declared testicular degeneration in G1 and G3 and a high spermatogenesis in G2 compared to control group. It was concluded that spirulina may act as health potentiator but also, may negatively affect the male reproductive performance. Special care should be paid for the dose and frequency of administration of any polyphenol or health potentioators.
Keywords | Spirul...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
...ost-thawing, progressive motility, plasma membrane integrity (PMI), and livability of buck spermatozoa were higher (P< 0.05) with 1mM GLU and 10mM CYS as compared to control. In the second experiment, post-thaw progressive motility, PMI, and livability were higher (P< 0.05) with 1mM GLU + 10mM CYS and 1mM GLU + 5mM CYS compared to other doses of GLU+CYS and control. We concluded that optimized supplementation of Glutat...
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
...uding the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63±16.54% and 52.76± 8.88%, and 53.00±7.93% and 54.08±8.76%, respectively. In swim-up method the average
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2
...n semen. Parameters were motility, motility progressive, kinematics sperms, viability, abnormality membrane integrity, and acrosome integrity. The Bali-polled bull semen was analyzed by using CASA with the Sperms Vision Version 3.7.5 program. The results in this study showed that sperms motility in Bali-horned bull was significantly lower (P<0.01) than Bali-polled bull, Brahman, Limousi...
Muhammad Kashif Maan1*, Rohma Shabbir2
...n, disturbance in ocular motility, visual deterioration, and chemosis. There was no evidence of presence of cyst in lungs and liver upon radiographic examination. Rhinotomy approach was used to enucleate the Cyst. There was no postoperative complication after enucleation of the Cyst.
Keywords | Exopthalmia, intraorbital hydatid cyst, proptosis, Rhinotomy
...
Idrissa Sacko1, Madou Dao1, Souleymane Sanogo1,2*, B. Michel Orounladji2, Kaly Diakité1, Diakaridia Traore3, Mamadou D. Coulibaly1, Amadou B. Cisse1
...gina. Semen volume, mass motility and live and dead sperm counts (spz) were measured by direct reading and electronic microscopy with a direct image transfer system on screen, respectively. Sperm concentration and total sperm count in ejaculate were determined by microscopy using single chamber Neubauer cell. Results show that ejaculate volume was influenced (p < 0.05) by individual (buck). Besides, individual and period of collection had no impact (p > ...
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
...color, consistency, mass motility, individual motility, spermatozoa concentration, viability, and libido. Data were analyzed using the ANOVA test. The results showed that the volume and pH of semen did not differ in all treatments, but treatment A showed cream color and thick consistency over treatments B and C. The mass motility and sperm concentration of treatment C with the dose and per...
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
...wing sperm parameters as motility, live, normal, and integrity acrosome up to 47.50, 65.10, 65.66, and 69.67%, but it was 37.22, 49.78, 55.89, and 59.22% relative to E0 extender, respectively. Furthermore, ATP, BC, and LPO levels post-thawing were 0.35µM, 255.40 CFU/ml, and 2.61 µM with E3 extender however, 0.19 µM, 276.00 CFU/ml, and 2.89 µM with E0 extender, respectively. Then, all of their previously enhanced post- thawing semen char...
Sri Suharyati, Siswanto, Madi Hartono, Kusuma Adhianto*
... or concentration, sperm motility, and vitality. L-carnitine is an amino acid compound and is able to provide energy so that it can help improve sperm quality. The objectives of this research were to find out the sperm quality of Jawarandu goats after oral administration of vitamin E and L-carnitine. The research used 16 Jawarandu male goats, with a completely randomized design (CRD) using 4 treatments, control (P0), administration of vitamin E 50 IU (P1), ad...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...showed that, progressive motility, live spermatozoa and acrosome integrity % were higher (P < 0.05) in G1, G2 and G3 with reduced mortality percentage than control group after cooling process. In addition, a significantly increased (P < 0.05) of progressive motility, acrosome integrity and alive spermatozoa percentage in groups treated with different concentrations of garlic extract than control group. While both the a...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...f concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls....
Xin Xing Dong1*, Gai Cui Bai2, Ming Li Li1, Guo Xiang Lan1, Da Wei Yan1, Xiao Yi Wang1, Qiang Chen1 and Shao Xiong Lu1*
...weight, gastrointestinal motility and growth hormone secretion. The aim of this study was to investigate the novel single nucleotide polymorphisms (SNPs) at GHRL gene in three different pig breeds, including Duroc, Yorkshire and Landrace, and the association of the novel SNPs with growth traits. PCR-SSCP and sequencing were used to detect the GHRL gene polymorphisms in the present study. Three SNPs were detected, one in the exon 1 (g.3734 G>T), which was sy...
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...cant change in the sperm motility, before freezing, post-thawing, and during recovery across age (p > 0.05). Semen volume, total sperm, total motile sperm, and frozen semen production increased in bulls from 6 to 7 years of age and then gradually reduced from 7 to 10 years of age. It could be concluded that the optimum semen productivity of Aceh cattle is when they are 7 years old.
Keywords | Artificial insemination, Frozen semen production, Indones...
Saleh M. Albarrak1*, Fahad S. Aldahmashi1,2, Abdulrhman A. Alrubayan1,2, Abdel Kader A. Zaki1,3
...(P ≤ 0.05). The sperm motility and viability were significantly higher in the >7 years group compared to the > 3 to ≤5 years and ≥5 to ≤7 years groups (P ≤ 0.05 and P ≤ 0.01, respectively). Significant differences were also observed between the examined two breeds concerning sperm viability and motility with sperm viability being higher in the Majaheem breed (P ≤ 0.05) and sperm
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
...ifying semen ejaculates [motility (>60%) volume (>4ml) and concentration (>0.5 billion/ml)] collected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was filled in 0.5 ml French straw at 4°C and...
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
...were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were motility, viability, abnormality, intact plasma membrane, acrosome status, DNA integrity, and movement patterns. Viability and abnormality parameters were stained with eosin-nigrosine, intact plasma membrane was stained with Hypo-osmotic (HOS) solution, acrosome status was ...
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
... size and percentages of motility, acrosome status, integrity of membrane, total sperm output, sperm concentration, sperm mitochondrial potential, and initial semen fructose. All PEE levels increased serum follicle-stimulating hormone, luteinizing hormone, testosterone, immunoglobulin, and antibody titer, while reduced lysozyme. All PEE levels improved antioxidants capacity, while reduced malondialdehyde in blood serum and in seminal plasma. Advanced
Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*
...n in the sperm count and motility with a parallel reduction in the acid phosphatase activity. It also directly reduced the concentration of free testosterone and estradiol in both sexes without any apparent effect on LH or FSH hormones. It increased the total cholesterol in males only and elevated the triacylglycerols and glucose levels in both sexes. The administration of testosterone to males, females, or both reduced the fertility index to zero. The mating ...
Ibrahim Samir Abd El-Hamid1, Alaa Emara Rabee2, Moustafa Mohamed M. A. Ghandour2, Rasha Salah Mohammed3, Ahmed Mohamed. Sallam4
...perm concentration, mass motility, sperm motility, sperm viability, and cell membrane integrity. Plasma testosterone concentration and number of red blood cells were higher (P≤0.05) in both treated groups. However alanine aminotransferase, cholesterol, total antioxidant capacity and glutathione peroxidase levels were reduced (P≤0.05) in both treated groups. It can be noted that, supplementing of plant herbs mixtures ha...
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
... spermatozoa progressive motility, visibility, and abnormality. Spermatozoa quality was observed every 24 hours until the spermatozoa motility reached at least 40%. The results showed that progressive motility and viability on the fourth day storage in extender Citrate-egg yolk (CEY) with 10% egg yolk and 10% GF were 50.4 ± 1.6% and 57.1 ± 1.6%, significantly higher (P<0.0...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...rmatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplementations were sequentially incubated for 15 minutes at 37 ºC and cooled at 5 ºC for 2 hour than packed in 0.25 ml straws, and cryopreserved in liquid nitrogen for 24 hours. Consequently, straws were thawed at 37C0 for 30 se...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
... can cause a decrease in motility, viability, and damage to plasma membranes that can reduce fertility rates. The use of antioxidants in semen preservation is very important to suppress lipid peroxidation so it can maintain the quality of the sperm. This study aimed to determine additional honey to the quality of KUB rooster sperm during cold storage. The semen with superior quality was diluted into four different treatments, lactate ringer egg yolk without ho...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
...rved included individual motility, viability, abnormalities, concentration, total motile spermatozoa, chromatin damage, intact acrosome hood of spermatozoa, and sperm capacitation. The treatments consisted of T1 = unsexed semen, T2 = sexed semen X-bearing sperm, and T3 = sexed semen Y-bearing sperm. A general linear model with three treatments and ten replications was performed in this research. The data were analyzed using a general linear model using SAS Dem...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
...ters measured were sperm motility, abnormality, viability, acrosome integrity, and membrane integrity. Liquid chromatography-mass spectrometry (LCMS/MS) analysis was used to assess the confirmation profile protein of seminal plasma. The conception rate from artificial insemination was used to determine the fertility rate (AI). This investigation revealed that the quality of the sperms from Bali-polled bulls and Bali-horned bulls did not substantially change af...
Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi
...les included spermatozoa motility, spermatozoa survival, spermatozoa abnormalities and intact plasma membranes of spermatozoa. The obtained data was analyzed using the Analysis of Variance (ANOVA). The results showed that the addition of tomato juice extract into egg yolk citrate diluent exhibited a 20% increase in sperm motility percentage, 26.3% in sperm survival rate and 22.5% in intact plasma membrane percentage as compa...
Doaa H. Assar1, Rasha A. Al-Wakeel2, Zizy I. Elbialy3, Mahmoud M. El-Maghraby4, Helmy K. Zaghlool5, Adel A. El-Badawy4, Abdel-Khalek E. Abdel-Khalek6*
...and percentages of sperm motility, livability, and abnormality as well as concentration and output of sperm cells were significantly by SPL. In serum and seminal plasma, glutathione, superoxide dismutase, catalase, and glutathione peroxidase significantly increased, while malondialdehyde significantly decreased in SPL groups. Based on our results, spirulina as a dietary feed additive (5 g/kg diet) could be used safely for improving growth, health, antioxidant ...
Hasanain Jihad Neamah
...≤0.05) enhanced sperm motility at 2 and 48 hour of cooling. A non-significant (p>0.05) increase in HOS-test percentage for all groups after 2 hours of cooling was observed. The PPE1 and PPE3 groups recorded the lowest percentage of HOS-test after 48 hours, however, groups PPE2 and the control recorded the significant (p≤0.05) excess in this characteristic. The sperm dead percentage were reduced by adding 10 mg of prickly pear extract (PPE2) in compar...
Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago
...e their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into 500µl of Ringer’s lactate solution with various concentrations of spermatozoa, namely 50×106, 100×106, and 150×106. Fertile period and fertility were evaluated by inseminating spermatozoa in hens. Eggs were collected two days after insemination and observed for embryo ...
Lalu Ahmad Zaenuri*, Rodiah Rodiah, Adji Santoso Dradjat, Oscar Yanuarianto, I Wayan Lanus Sumadiasa , Lukman Hy
..., viability, progressive motility, and morphology, including normal and abnormal spermatozoa. The statistical significance of the results was evaluated by pre- and post-test analyses using T-test with repeated measuring. Data were expressed as Means ± SD and SEM. Results revealed that semen volume, spermatozoa concentration/ejaculate, plasma membrane integrity, viability and progressive motility, and normal spermatozo...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
... four treatments, namely motility before freezing (70.86%) and post thawing (53.00%); viability before freezing (76.00%) and post thawing (60. 14%); plasma membrane integrity before freezing (71.29%) and post thawing (59.86%); and also showed the lowest abnormality level before freezing and post-thawing, (17.29%) and (24.14%), respectively.
Keywords | Spermatozoa, Belgian blue crossbreed, Egg yolk citrate, Lycopene
...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
...sh;egg yolk. Progressive motility post-thawing at 1 hour and 2 hours was highest at genistein concentration 30 µM (P<0.05). The effect of the antioxidant genistein on tris aminomethane - egg yolk diluent gave a significant effect on sperm viability in the Before Freezing (BF) phase at 1.5 hours, BF and PT at two hours (P<0.05). The addition of different genistein in diluent tris aminomethane - egg yolk was no significant effect (P>0.05) on sperm...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
...sperm analyser for sperm motility parameters, and by confocal laser scanning microscope for sperm membrane integrity parameters. Sperm lipid peroxidation was also analysed through malondialdehyde production. The study found that there was a gradual decrease in all sperm quality indicators of all the treatments during 12 days of storage. In addition, the chilled sperm motility parameters among all the semen extenders were not...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
...al microscope to analyze motility and membrane properties, respectively. Additionally, malondialdehyde production was measured to evaluate lipid peroxidation capacity. The results revealed that incorporating essential oils at concentrations under 100 µg/ml in semen extenders had a positive impact on sperm quality. Nonetheless, employing essential oils at elevated concentrations of 200 µg/ml exhibited detrimental impacts on the sperm. The optimal do...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm percentage, and level of total antioxidant capacity and superoxide dismutase activity, and decreased percentage of apoptosis, early apoptosis, necrosis, and malondialdehyde level; most favorable results were recorded with PEE2. The foregoing results indicated that supplemen...
Moustafa M. Zeitoun 1,3; Mohamed M. Mansour 2,4*
...od aldosterone and sperm motility percentage. Besides, plasma aldosterone levels in infertile animals were positively correlated (r = 0.26) with sperm abnormality ratios. Conclusion; The blood and seminal plasma aldosterone and testosterone levels could be used as a biomarker to predict fertility in male dromedary camels. Further, it could recommend measuring blood aldosterone relative to seminal plasma testosterone for early diagnosis of sub-fertility in male...
Umar Salisu Ahmad1, Adamu Abdul Abubakar1,2*, Hassan Abubakar Bodinga1, Nura Abubakar1, Ekaete Ime Oviawe1, Zaid Shehu3, Abubakar Musa Mayaki3
... observed, reduced rumen motility, bilateral ocular discharge, foamy salivation, and labored breathing were observed. The prescapular and submandibular lymph nodes were enlarged, and a hard mass was palpable on the distended abdomen. Rectal examination revealed hard rectal feces in rectum were found. The rectal temperature was subnormal (35.4 oC), respiratory rates were 12 cycle/min and the pulse rate was so weak that it was not detectable. Attempts to induce ...
Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2
...ity (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentage of motility, percentage of live spermatozoa, and intact plasma membrane. The NEZ group showed better results (P<0.05) than the NEZ group in maintaining motility, viability, and membrane integrity. Th...
Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2
...ers included progressive motility, viability, and abnormalities of spermatozoa. The data obtained were analyzed using the analysis of variance (ANOVA) followed by Duncan’s multiple range test to determine the difference among the treatments. The current study showed that adding 15% sugarcane (T2) improved the semen parameters compared to the other treatments with 54% progressive motility, 74.8% viability, and 8.8% tota...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...eters such as viability, motility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the diluent before freezing exhibited significantly higher (P < 0.05) spermatozoa motility. Moreover, the viability and motility of post-thawed semen significantly improved (P < 0.05) at lycopene doses of 1% and 2%. The addition of 1% to 2% ...
Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4
...sured in this study were motility, viability, abnormality, membrane integrity, acrosome integrity, movement pattern and proportion of spermatozoa. The data obtained were analysed using one-way analysis of variance (ANOVA). A Descriptive Statistical test used to analyze the proportion of sperms. The results showed that different concentrations of sexing media showed no significant difference (P>0.05) on motility, viability...
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
...esults showed that sperm motility, viability, and plasma membrane integrity were significantly higher (P<0.01) when 2-3 mM BHT was added, while acrosome integrity was elevated after the addition of 1 and 3 mM BHT. Furthermore, the addition of BHT did not significantly change semen pH. DNA damage was lower (P<0.05) when 3 mM BHT was added and mitochondrial activity was enhanced under the same condition. The concentration of malondialdehyde (MDA) was lower...
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
...ution ratio, the highest motility duration of 74.3±2.16 s was obtained at 1:10 dilution ratio. The best results on motility duration (62.28 ± 2.12 s), fertilizing ability (77.3±1.63 %) and hatching rate (61.6±2.44 %) was recorded when freezing protocol I was followed and the values were statistically significant. Hence the results revealed that for successful cryopreservation of C. carpio milt, us...
ABRAR HUSSAIN1, AQSA KHALIL1, SAIRA ZAHEER1, MUHAMMAD LATEEF2 & MUHAMMAD MANSHA1
...CEE) was prepared. Adult motility assay (AMA) was carried out on adult Haemonchus contortus to analyze the anthelmintic efficacy of CEE of the whole plant and the leaves of Tribulus terrestris L and Ziziphus mauritiana Lam. The motility of insects was assessed at concentration of 2, 4, 6 & 8 mg/ml after 2, 4, 6 & 8 hours of post treatment and dead worms were noted. The 8mg/ml concentration of CEE of Tribulus terrestr...
Aziz Ahmed1*, Anila Naz Soomro2, Muhammad Ramzan Ali1, Muhammad Aleem Khan1 and Hasina Basharat1
...parameters i.e., percent motility, motility interval, sustainability, and fertilization rates in Nile tilapia (Oreochromis niloticus). The experimental design was CRD with four treatments and three replicates. Lab prepared (Activation media A and B), one commercially available (Activation media C) and freshwater served as control. After the pre-developed broods were selected based on maturity approaches and collected sperm s...
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
...ly. The sperm used had a motility of over 80%. The semen was diluted Lactate Ringer with 10% egg yolk and the addition of 7% DMSO cryoprotectant. Then the semen was equilibrated at different times and then frozen. The frozen semen was then stored in a liquid nitrogen container at a temperature of -196°C.After 24 hours, the semen was thawing at a different temperature. The evaluation of semen quality included motility, vi...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...stress and affects sperm motility. Antioxidants in the diluent can maintain the motility of bull sperm during the cooling process until after thawing. This research examines the effect of Myrmecodia extract (MYE) supplementation, which has high antioxidants, on semen diluent during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice w...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...aculates having ≥ 80% motility, morphology, and live-dead ratio were pooled and diluted in Tris-based Egg Yolk (TEY) extender supplemented with various concentrations of selenium, i.e., group A control (0mM), group B (2mM), group C (4mM) and group D (6mM). Results showed significantly (P<0.05) higher motility (78.1±0.22), morphology (86.5±1.08), membrane integrity (85.6±0.30) and live dead ratio (8...
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