Muhammad Nawaz Kandhro, Qamaruddin Jogi, Mahmooda Buriro, Aijaz Ahmed Soomro, Ghulam Mustafa Laghari and Ali Nawaz Khaskheli
...kg-1 soil showed highest inhibitory effect. In both aforesaid treatments, lowest and statistically similar values of germination, root/ shoot length and fresh/ dry weight seedling-1 were recorded for both C. arvensis and C. rotundus, respectively. The water extract of E. camaldulensis showed superiority in allelopathic effects over its powder. Inhibitory effect of water extract or powder increased at higher rate (30 ml or 30...
Shakeel Ahmed* and Sarwat Jahan
...dipokines secretion. Its inhibitory effect on leptin and stimulatory effect on resistin shows an important relationship between NMS and adipokines in the regulation of reproductive axis in male rhesus monkeys. Further studies are required to confirm this relationship....
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
...sitive controls. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts was also determined. Syzygium aromaticum was proven to have excellent antimicrobial activity against all tested microorganisms, while C. sinensis and M. piperita showed weak antimicrobial activity. Methanolic plant extracts exhibited greater antimicrobial activity than aqueous extracts. Maximum zone of inhibition exhibited by methanolic extrac...
Sidra-Tul-Muntaha1, Muhammad Sagheer1*, Mansoor-ul-Hasan1 and Shahbaz Talib Sahi2
...the repellent and growth inhibitory efficiency of five plant extracts (Azadirachta indica, Melia azadirach, Pegnum hermala, baryosma and Zingiber officinale) and three synthetic pyrethroids (bifenthrin, cypermethrin and deltamethrin) on three geographical populations of Callosobruchus chinensis collected during 2013 from Faisalabad, Multan and Nankana districts of Punjab, Pakistan. Three concentrations of each plant extract (5, 10, 15 and 20%) and synthetic p...
Antibacterial Activity by Chlorogenic Acid Isolated through Resin from Apricot (Prunus Armeniaca L.)
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...al activity. The minimum inhibitory concentration of isolated compound was also measured. Two antibiotics were used as references compounds for antibacterial activities. The aqueous ethanolic extract having 968.125 µg/ml of chlorogenic acid inhibited significantly the growth of tested bacterial strains. Among the bacterial strain tested Salmonella entritidis and Helicobacter pylori were even more sensitive to 20.313 (µg/ml) of chlorog...
M. B. Meah
Biopesticides for crop growth and crop protection
...ablets displayed similar inhibitory action against damping-off and seedling blight pathogens,Phomopsis vexans and Sclerotium rolfsii. In the field, garlic and allamanda spray reduced leaf blight severity by 84-86% and fruit rot by 91%. Severities of nematode and virus infection were also reduced significantly with allamanda spray. Active compounds in allamanda leaves have been separated and identified.Formulated trichoderma effectively controlled soil-borne di...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
... of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin. Camel raw milk produced 2.4 mg/mL of lactoferrin. The lactoferrin isolated from camel milk exhibited promising antibacterial activity against E. coli and Staphylococcus aureus. Concentration of lactoferrin i.e. 4,000 ...
Saba Irshad1,*, Ayesha Ashfaq1, Ammara Muazzam1 and Abida Yasmeen2
...s up to 35%, with no inhibitory influence on Candida species and F. solani. These extracts fully inhibit growth of PC3 cells. Moreover, partially purified lectin, from Curcuma longa L. with a molecular weight of 17.3 KDa might be useful to treat patients as a considerably cheap herbal drug which can be prescribed to poor people efficiently at an affordable cost.
... 
Maryum Fakhar, Iqra Jabbar, Anjum Nasim Sabri*
...evealed that at low/ sub-inhibitory concentration of nickel (500 μg/ml), motility is inhibited; adhesion, hydrophobicity, aggregation, EPS production and biofilm formation of two strains are increased. This biofilm forming ability can be employed in
urification of waste waters and treating contaminated soils....
urification of waste waters and treating contaminated soils....
Asma Akhtar, Abdul Rehman
...NA sequence. The minimum inhibitory concentration of both isolates showed fair growth up to 1 mM of K2Te2O3. Both strains showed maximum growth at 37ºC and pH of 7. The bacterial growth in the presence of metal ions was delayed as compared to the control. High ellurite reduction potential was shown 98% (1f) and 97% (2b) after 24 h of incubation. Glucose 6 phosphate dehydrogenase (G6PDH) activity was increased to 279% (1f) and 150% (2b) when ompared to the...
Amtul Jamil Sami1,*, Sehrish Bilal1, Madeeha Khalid1, Muhammad Tahir Nazir1 and A.R. Shakoori2
Shahida Khalid*and Shahida Nasreen Khokhar*
INTERACTION OF HERBICIDES AND BIO-INOCULANTS WITH AGRICULTURAL CROPS AND WEEDS
...irillum) to mitigate the inhibitory effect of herbicide residue in soil, and their inhibitory effect on weed seeds. The crops studied under laboratory conditions were Brassica spp. and Zea mays while Phalaris minor was the test weed. In first experiment, Brassica and maize, grown in soils having residues of either Triasulfuron+Terbutryn (Logran) or Sulfosulfuron (Leader), were found to significantly mitigate (% increase in r...
Muhammad Mudasar Aslam ⃰, Muhammad Jamil**, Ijaz Malook**, Amana Khatoon*, Ali Rehman*, Abdur Rahim***, Pirzada Khan*, Shakir Ullah Khan Shakir*, Shahid Irfan*, Faizan Ullah****, Khair Ul Bashar**, Mahideen Afridi** and Shafiq Ur Rehman*
PHYTOTOXIC EFFECTS OF CALOTROPIS PROCERA, TAMARIX APHYLLA AND PEGANUM HARMALA ON PLANT GROWTH OF WHEAT AND MUSTARD
...t is thus concluded that inhibitory effect increases with the increase of extracts concentration....
Abdul Rehman Khan*, Muhammad Mahmood–Ul-Hassan**, Rizwan Ahmad** and Anjum Munir ***
ARSENIC III TOLERANCE POTENTIAL OF FUNGI ISOLATED FROM POLLUTED AND NON-POLLUTED SOILS OF PAKISTAN
...ance index (TI), minimum inhibitory concentrations (MIC) and growth rate. Out of 18 isolates, 12 belonged to genus , 3 to , 2 to and one to . The isolates and appeared to be most tolerant to arsenic. Fungal strains isolated from Gujranwala soil exhibited more As(III) tolerance than those from Multan and Islamabad. Maximum fungal growth was observed at temperature of 30 to 35 C and pH 6 to 7 under incubation period of 96-120 hrs. The isolated strains were capab...
Asif ullah Khan*, Faizan Ullah*, Sultan Mehmood*, Muhammad Irshad* and Farhat Ullah Khan**
ALLELOPATHIC EFFECTS OF JATROPHA CURCAS L. LEAF AQUEOUS EXTRACT ON EARLY SEEDLING GROWTH OF L. PARTHENIUM HYSTEROPHORUS
...eous extract of exhibits inhibitory effects on growth and development of and therefore could be a potential source of botanical herbicide in the control of this noxious weed species....
Muhammad Sarfraz*, Shahida Hasnain
...actamases was done using inhibitory based method and confirmed 46% K. pneumoniae and 8% E. coli pAmpC positive. A variable resistance pattern was seen in both pAmpC beta-lactamases producing K. pneumoniae and E. coli for Amikacin, Gentamicin, Levofloxacin, Ciprofloxacin, Imipenem, Meropenem, Pipracillin-Tazobactam, Cefoperazone-Sulbactam and Cefepime. The study elaborates recent trends in microbial profiles of pAmpC beta-lactamase p...
Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1
...ptomycin had almost same inhibitory zone that showed its effective use in food. In general, results showed that encapsulation is an effective approach for protection of active ingredients of pumpkin seed oil. ...
Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*
Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
...ere more potent. Minimum inhibitory concentration of heavy metals for all bacterial isolates was different. It was concluded that irrigated pathogenic bacteria are highly resistant to heavy metals....
Atiq-ur-Rehman1,2,*, Abida Latif1,*, Rukhsana Anwar1, Nasir Abbas1, Sajid Nawaz2 and Hamid Turab Mirza3
...gated by α-amylase inhibitory assay. Different concentrations of fractions (20-100 µg/ml) were subjected to in vitro α-amylase inhibitory assay. Assessment of anti-hyperglycemic potential of the most active chloroform fraction exhibiting in vitro α-amylase inhibition was carried out in Wistar albino rat models. Changes in postprandial levels of blood glucose in rat models (normal and dia...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...nic detergents showed an inhibitory effect on the enzyme activity. Kinetic studies of recombinant protein demonstrated the km and Vmax values of 0.22 mg/mL and 2500 µmoles/min respectively with the specific activity of 12464 Umg-1, using carboxymethyl cellulose as substrate. The deinking potential of recombinant cellulase to remove ink from the paper makes this enzyme a suitable candidate for its use in pape...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
...all, while comparing all inhibitory compounds trypsin was most effective in decreasing biofilm formation followed by chymotrypsin > proteinase k > salicylidene acylhydrazides. Our study indicated that inhibitory compounds may disrupt the protein filament of flagellar apparatus producing significantly controlled biofilm formation which might also be helpful to control clinical infections associated with Desulfovibrio...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...owed different degree of inhibitory effect. The inhibitory effect of the protein monomer on gram-positive bacteria was stronger than that on gram-negative bacteria, but not on candida albicans. This novel and monomeric APP-1 has antibacterial activities and imply that it is likely an important component of antibacterial activity in antler plate polypeptide.
... 
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...timicrobial agent having inhibitory effect on serine/threonine phosphatases leading to hindrance in bacterial growth. In addition, phylogenetic tree was also constructed using 18s rRNA gene of A. niger and gene clusters of secondary metabolites were determined through bioinformatics tools.
...
Naila Riaz1, Hafiz Muhammad Tahir2, Muhammad Khalid Mukhtar1, Ali Hassan2, Shaukat Ali2, Shafaat Yar Khan1
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
... In second step, minimum inhibitory concentration of test compounds was determined against bacterial species by using resazurin microplate assay. All the tested compounds exhibited variable antimicrobial properties against various test cultures. All the compounds showed stronger antimicrobial activity against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Compound III was found to be the most effective compound. These results de...
Umer Iqbal1,* and Tariq Mukhtar2
.... Highly significant inhibitory effects of fungicides were recorded on the growth of M. phaseolina. All the fungicides caused significant inhibition of the fungus over control. Maximum individual inhibition of growth of the fungus was recorded with Benomyl (83.89%) followed by Carbendazim (79.11%) at a concentration of 150 ppm. Copper oxychloride at a concentration of 50 ppm gave the minimum inhibition (12.50%). Concentrations also had significant <...
Boxin Dou, Ying Liu*, Yumeng Liu, Lili Fan, Yongqiang Ma, and Yanguo Shi
...tionated and the new ACE inhibitory triplet was isolated and identified. The amino acid sequence of the ACEI was identified as Ile-Thr-Leu or Leu-Thr-Ile. In vitro, ACE inhibition assays showed that the IC50 value of the peptide was 0.0118 mg.ml-1. The inhibitory activity of the peptide slightly increased after incubation with gastrointestinal proteases. It was revealed that the ACE in...
Abida Parveen1, Sajid Aleem Khan1, Nazir Javed1, Anam Moosa1*, Khushboo Javaid1, Hina Safdar1 and Asma Safdar2
...tion. In this study, the inhibitory effect of Moringa oleifera L. (MO) extracts and Steinernema kraussei Steiner (SK) as stand-alone treatments and in combination was assessed to suppress the reproduction and development of MI. Highly susceptible variety of tomato cv. ‘Money Maker’ was tested for the invasion and development of MI. Aqueous and ethanolic MO extracts were tested in combination with SK at three different concentrations a...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3
...antimicrobial by minimum inhibitory concentration (MIC) and minimum mycoplasmacidal concentration (MMC) value was ciprofloxacin having lowest MIC and MMC value (0.390 µg/ml and 0.781 µg/ml), respectively. It was concluded that ciprofloxacin had best mycoplasmacidal and mycoplasmastatic activities among tested chemotherapeutics agents against Mccp. Ciprofloxacin could be more effective antimicrobial in field against Mccp infection in goat.
Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu*
...nizing quercetin’s inhibitory effect on airway inflammation in mice and its possible mechanism of action. The mice received varying doses of quercetin from 22-30 days (1, 10 and 50 mg / kg, p.o.) and montelukast (10 mg / kg, p.o.). Intranasal OVA has been instilled on the 21 days. Biochemical parameters, spleen weight, physiological parameters, interleukin (IL-1β and IL-6) parameters and immunoglobin-E (IgE) were calculated at the end of the experim...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2, Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6
...n after 1 hour, however, inhibitory effect of proteinase k was more pronounced (p<0.001; 80%) compared to the other two enzymes (45% and 34 % respectively). Hence, we concluded from this study, that S. Gallinarum is a strong biofilm former. Use of proteases can strongly inhibit biofilm formation in vitro and can be used as effective therapeutic approach to control fowl typhoid epidemic along with an antibiotic therapy. The future prospects of ...
Ch. Muhammad Rafiq1, Qasim Raza1*, Awais Riaz1, Misbah Hanif2, Wajiha Saeed2, Shawaiz Iqbal1, Tahir Hussain Awan1, Syed Sultan Ali1 and Muhammad Sabar1
...SA treatments alleviated inhibitory effects of different osmotic stress levels after 2–4 days of stress applications. Under medium to high osmotic stress levels, mean germination time, germination index and seed vigour index (SVI) of SA primed seeds were better than non-primed seeds. Remarkably, SVI of all SA concentrations under -0.2 MPa osmotic stress was surprisingly improved as compared with control and other osmotic stress levels, indicating a &lsqu...
Qaiser Shakeel1*, Rabia Tahir Bajwa1, Yasir Iftikhar2, Mustansar Mubeen2, Muhammad Luqman3, Waqas Ashraf1 and Ifrah Rashid4
...e plants showed a better inhibitory effects on the pathogen’s (P. digitatum) mycelial growth rather than fungicides. Among all the plant extracts, the best result was showed by garlic extract with 87% of mycelial growth inhibition followed by moringa with 82%, the best concentration is 100mg/mL. Besides, Ridomil gold showed the most effective control against ginger soft rot with 84% of mycelial inhibition growth among all the tested fungicides. And found...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
... guidelines. The minimum inhibitory concentration of ESBL producing isolates was determined by the broth dilution method. Phenotypically confirmed ESBL producing strains were further subjected to molecular characterization for the presence of ESBL and carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL positive E. coli were recovered and among these 156/213 (73.2%), 42/63 (66.6%) and 21/27 (77.7%) were from poultry, environmental water, an...
D. N. Abd-elshafy1, 2 and M. M. Bahgat2, 3
...cellular metalloprotease inhibitory effect that enhances rate of HAV replication and thus reduces time needed for the virus to cause cytopathic effect. Such finding will definitely facilitate the detection of the virus both in clinical or environmental samples using cell culture techniques....
Radwa M. Shafie 1 and Soha S. M. Mostafa2
...re determined....
Dina N. Abd-Elshafy
...stability
of inhibitory effect of extract on HAV in water.
Results: Green tea extract has a strong anti-HAV effect with direct effect on the viral
particles. Supplementing tap water with the extract with continuous shaking every 15 minutes
for 1 hour caused more reduction in the percentage of HAV plaque counts compared to
allowing the supplemented water to rest for without shaking and the same effect ...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...Bauer method and minimum inhibitory concentration (MIC) to aminoglycosides was evaluated by the broth microdilution method. Moreover, the armA genes were studied by PCR followed by Sanger sequencing. The isolates showed a high rate of resistance to cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones whereas colistin and tigecycline were the most active antimicrobial agents against A. baumannii as none of the isolates was found resi...
Hafiz Muhammad Tahir*, Iram Liaqat*, Junaid Nadeem, Hammad Aamir and Shaukat Ali
...r failed to consider the inhibitory effects of the processing chemicals or did not investigate whether the inhibitory potential is an intrinsic property of silk or is due to the aggregate gland secretion coating its surface, hence a borrowed property. Our study used direction application and disk diffusion method to investigate the antibacterial potential of Neoscona mukherji and N. theisi egg and web silk. For disk diffusio...
Rasha Ali Taha Hamza1, Atef Saad Osheba1, Hassan Mohamed Sobhy2, Sahar Hussein Abdalla Hekal2*
... of meat. The results of inhibitory effect against the selected organisms indicated that; 90% ethanolic extract of sumac and galangal was found to be effective against all Gram negative bacteria, Gram positive bacteria, yeast and molds, with note that the gram positive bacteria were more sensitive than gram negative. Antibacterial activity of different galangal and sumac extracts against total bacterial count was more effective in burger prepared with Sudanese...
Muhammad Asif1*, Ahmad Ali Shahid1,2 and Nasir Ahmad3
... prepared to observe the inhibitory effect of G. lucidum against A. solani. The results for interaction of mycelia of G. lucidum with pathogen A. solani reveal that G. lucidum is an efficient biocontrol agent to reduce the disease incidence. Crude protein extract was also found beneficial to control the pathogen. The results for minimum inhibitory concentration (MIC) of crude protein extract reveal that 5mg/ml is the concent...
Jiang Wu*
...ar diffusion method. Sub-inhibitory concentration (SIC) value, minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value were measured. The virulence gene expression of the four foodborne pathogens was analyzed by quantitative real-time PCR (qRT-PCR). A microplate reader was used to monitor cell growth at 600 nm with 1 h interval. Environmental scanning electron microscope (ESEM) was use...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
...ignificant enhancing and inhibitory effect upon different concentrations. Effect of inhibitors (β-mercaptoethanol, EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucan...
Qiong Xiang1, Jing-Jing Li1, Qian Zhang1,3, Rong-Bo Tian1 and Xian-Hui Li1,2*
Amjed Ali1, Muhammad Tayyab1*, Abu Saeed Hashmi2, Asif Nadeem3, Shumaila Hanif4, Sehrish Firyal1, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1
...detergents showed slight inhibitory impact on the recombinant β-lactamase activity. The enzyme exhibited the Km and Vmax values of 2.27 mM and 45.45µmol/min, respectively when benzylpenicillin was utilized as substrate. The degradative ability of recombinant β-lactamase to hydrolyze a variety of β-lactam ring containing antibiotics makes it a suitable candidate for its utilization as positive control in diagnostics and in antibiotic suscep...
Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby*
...y, while in MIC (minimum inhibitory concentration) method 68.8% exhibited resistance. Colistin was recorded as 33.7% in chicken breast by HPLC. Furthermore, mcr-1gene was detected 54% using PCR. Sanger sequencing revealed the same identity (100%) between the three examined strains despite coming from different sources. It could be concluded that the practices inside poultry farms might be a possible source of spread of antimicrobial resistance to the food chai...
Gamilat A. Elsaid1, Weam Mohamed Baher1*, Eman Shukry1, Abeer E. Abd El. Ghafar2, Marwa Shalaby1
...using PCR. Moreover, the inhibitory effects of potassium sorbate (KS) and Bifidobacterium animalis subsp. lactis on the growth of E. coli O26 were screened. The estimated MPN of coliforms and E. coli in kareish cheese collected from grocery stores were 3.44 ± 0.12 and 2.22 ± 0.14. These values were 4.61 ± 0.11 and 3.60 ± 0.25 in samples collected from street vendors. The prevalence rates of E. coli were 20%, and 60% in kareish chees...
Iqtidar Hussain1*, Shakeel Ahmad Jatoi2, Muhammad Jawad Nazir1, Ehtesham Ul Haq1, Faheem Abbas1 and Muhammad Saqib Raza Shah1
...eous extract produced an inhibitory effect on all the crops’ growth parameters studied. It is recommended that unplanned planting of poplar (P. deltoides L.) around the cereal crop fields should be avoided in order to obtain optimum crop population and finally the grain yield....
Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3
...ensitivity test, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration were determined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyz...
Mehwish Saleem1* and Amina Atiq2
...rlic nanoparticles, with inhibitory zones of 19 mm, 17 mm, and 18 mm for an aqueous extract for aqueous extract and for ethanolic garlic extract was (19mm, 17mm and 15mm) K. pneumonia was the least susceptible, with inhibitory zones for aqueous and ethanolic garlic extracts of 10 mm, 9 mm, and 11 mm, respectively (10mm, 8mm and 10mm). The findings of the study point to the potential for supplementing antibiotic therapy with ...
Sayed H. Al Habty1, Dina N.Ali2*
Aqsa Javaid, Masoom Yasinzai* and Khunsa Saeed Kiyani
... 2+ metal ions while the inhibitory effect of Ca 2+, Co 2+, Zn 2+, Hg2+ and Fe2+ was recorded with 27.12%, 32%, 57.31%, 14.76% and 13.54% residual activity....
M. S. Bajestani1, K. Dolatabadi2†, E. Mahdikhani-Moghadam1
...>...
A.M. El-Baha1, A. A. El-Sherbiny2†, M. Z. M. Salem1, N. Shaarawy3 and N. H. Mohamed3
...howed median
inhibitory concentration (IC50) values of 412.7 and 615.9 mg/l for C. citriodora and E. camaldulensis leaves EOs,
respectively. In addition, the lethal concentrations (LC) causing 50% J2 mortality (LC50) of C. citriodora and E.
camaldulensis oils were 235.9 and 327.7 mg/l, for C. citriodora and E. camaldulensis leaves EOs, respectively.
Essetial oils were analyzed by gas chromatography-mass spectrome...
A. S. A. Saad1, M. B. Al-Kadi1, A. A. A. Deeabes2 and A. M. El-Kholy2†
...icide for their hatching inhibitory and juvenile mortality potential and to ascertain their role as organic amendments for the management of root-knot nematode (Meloidogyne incognita) affecting okra plants in vitro as well as in vivo conditions. Five concentrations of all chemicals were prepared. Data of in vitro studies showed a marked nematicidal and nematode hatching inhibitory activity against root-knot nematode (M. inco...
W.M.A. El-Nagdi†, M.M.A. Youssef and M.G. Dawood*
...al and nematode-hatching inhibitory activity reduced nematode criteria including
number of galls, egg-masses and hatched juveniles on roots of eggplant and number of juveniles in soil at harvest
stage compared to untreated plants significantly (p ≤ 0.05). Lower concentration of the tested materials caused
higher percentages reduction the mentioned nematode criteria. Vice versa, increase in length of shoots, dry and fresh<...
Salman Hussain1, Basit Zeshan2*, Rabiya Arshad1, Saba Kabir1 and Naveed Ahmed3
..., and vancomycin minimum inhibitory concentration (MIC) E-test. The MRSA isolates were detected for the presence of mecA, mecC, and nuc genes. Congo red agar (CRA) method was used to assess the ability of isolates to form biofilms. The results of the study showed that the prevalence of MRSA was 48%. The MRSA isolates were highly resistant (100%) to penicillin, β lactamase inhibitors, cephalosporins, and macrolides. All the MRSA isolates were susceptible t...
Dina N. Ali1*, Sayed H. Elhabtey2*, Manal M. Amin1
...sion method. The minimum inhibitory concentration (MIC) of MO and Nano-MO was 3% and 2%, on Listerea monocytogenes, Staphylococcus aureus, Salmonella typhi and E. coli bacteria, respectively. While, in inoculation of MO and Nano-MO in cheese complete reduction of examined bacteria was recorded at 3rd week of storage except for L. monocytogenes Nano-MO killed it at 2nd week. Overall acceptability (OAA) investigations showed better results of Nano-MO than MO. Na...
SYED ALI RAZA1, MUHAMMAD WASEEM MUMTAZ2*, AYOUB RASHID CH1, ABDUL WAHEED1, JAMES WILLIAM3 & MUHAMMAD ARSHAD1
...nt and pancreatic lipase inhibitory properties of different hydroethanolic leaf extracts of Conocarpus lancifolius. Freeze drying assisted ultra-sonication was adopted for extract preparation. The results showed that highest total antioxidant power of 291 ± 0.50 mg ASE/g DE was exhibited by 60% ethanolic extract which was slightly lower than antioxidant power of BHA (309.16 ± 3.90 mg ASE/g DE). The β-carotene bleaching inhibition of 8...
Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3
...ytotoxicity and in vitro inhibitory activities of local medicinal plant Azadirachta indica (A. indica) extracts on expression of NS3 and NS5A nonstructural proteins of HCV 3a genotype. Owing to the fact that HCV patients can develop HCC, plant extract has also been tested for cytotoxic activity. Colorimetric analysis was done to determine the vitality of HepG 2 cells for 24 h after treatment with methanolic seed extract and minimum inh...
Chenchen Liu1,2, Tong Wang1, Muhammad Khan3*, Xiaolin Cui2 and Yongming Li1,2*
...ian cancer cells. Growth inhibitory effects of ALT are evident from CCK-8, Edu, and colonogenic assays. The molecular mechanism associated with induction of apoptosis includes ROS generation, G2/M phase arrest and inhibition of aerobic glycolysis. Inhibition of glycolysis was found to be linked with down-regulation of lactate dehydrogenase A (LDHA) and glucose transporter-1 (GLUT1). Finally, ALT effectively suppressed growth and induced apoptosis in cisplatin ...
Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*
.... Meanwhile, the minimal inhibitory concentration (MIC) for amikacin and norfloxacin is significantly reduced from 64 µg/mL to 1 µg/mL and from 256 µg/mL to 8µg/mL respectively with frictional inhibitory concentration (FIC) index 0.25. Therefore, the FIC index recognized a synergistic activity between amikacin and norfloxacin against all P. areuginosa isolates. The mPCR was an efficient tool for detec...
E. K. Allam.1; B. A. Othman.1; Hayam S. Abdelkader2; and Noher A. Mahmoud2
...t was suggested that the inhibitory compounds released during the extraction of RNA constitute a limiting factor for the detection of GFLV in infected vines. Both immunological and molecular detection methods provide tools assisting in the understanding of the epidemiology and diversity of nepoviruses as well as to facilitate resistance breeding, cultivar selection, and development of control strategies....
Muhammad Afzal Naeem1*, Muhammad Hassaan Khan2, Tahmina Nazish3, Uzma Bashir4 and Midrarullah5
... found to impart maximum inhibitory effect on the germination indices of the wheat (T. aestivum) followed by the stem extracts, as the highest concentration of ACWE (i.e. 100%) caused the reduction of 45.65, 54.55, 50.9, 61.6, 5.3 and 9.4 % in germination percentage, germination energy, germination index, vigour index, germination coefficient velocity and relative percentage of wheat, respectively, compared to the control. Delay of 2.98 % mean germination time...
Muhammad Ather Nadeem1, Bilal Ahmad Khan1,2*, Neelam Yaqoob3*, Abdullah2, Amina Iftikhar4, Muhammad Irfan5, Hafiza Zara Saeed6, Syed Saqlain Hussain7 and Sidra Shakil8
...es. It was observed that inhibitory effect depends on the level of concentration. ...
Maria Azam1, Tahir Mahmood Qureshi1, Saddam Hussain2, Amjad Islam Aqib3*, Shanza Rauf Khan4, Kashif Akram1, Misbah Ijaz5, Maheen Murtaza6, Afshan Muneer6 and Sammina Mahmood7
...ontribute lowest Minimum inhibitory concentration (MIC) (1.302±0.564 mg/mL) followed by Fe2O3 (2.930±1.691 mg/mL) and ZnO (3.906±0.000 mg/mL). MIC of ZnO was recorded to be 18.23±11.93 mg/mL, 15.63±0.0 mg/mL, 13.02±4.5 mg/mL, 7.81±6.77 mg/mL, 5.21±2.26 mg/mL, and (3.906±0.000 mg/mL) at 04, 08, 12, 16, 20 and 24th h of incubation. A similar trend was followed by other nanoparticles at different hour...
Tabark F Ahmed, Nibras Z. Yahya*
...fects through fractional inhibitory concentration (FIC index < 1) against tested isolate, while the combination of ascorbic acid with erythromycin exhibited antagonistic effects. Furthermore, time-kill analysis showed that ASCORBIC/CIP combination exhibited highest synergistic and bactericidal effect. It was determined that in vitro study against E. coli O157 in the treatment of urinary tract infection, the combination of ascorbic acid and ciprofloxacin was...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...e potential. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of screened Actinomycetes was in the range of 1.3 - 3.5 mgmL-1 and 1.9 – 4.0 mgmL-1 of bacterial biomass, respectively. Three isolates, S. monticola, S. septentrionalis and S. polaris showed 100 % mortality at 1000 ppm against Anopheles 3rd instar larvae. These findings indicated that actinobacterial isolates possess antibacterial and larvicidal potential...
Chen Zhao, Lulu Ding, Ying Ye, Congying Kou, Haoran Xiao, Jing Zhu and Jicang Wang*
...g that Nar has a certain inhibitory effect on cell autophagy. In general, this study shows that Cd caused oxidative stress and autophagy. Nar can reduce the toxicity of Cd by attenuating oxidative stress and inhibiting autophagy. The specific mechanism of Nar antagonizing the toxicity of Cd can provide new ideas for anti-Cd toxicity, and also provide theoretical basis for the clinical application of Nar....
Raed Hussein Salih Rabee1, Yahya Sabah Abdulameer1, Walla Farhan Obed1, Noor R Abady1, Adnan Mansour Jasim1*, Firas Hussein Albawi1, Mohammed Jasim Jawad2, Ahmed Samir Abukhomra3
... docking study confirmed inhibitory activity of Cytochrome P450 3A4 (CYP3A4) and P-glycoprotein (P-gp) an enzyme responsible for the metabolism of antibiotics. Additionally, berberine demonstrated antibacterial effects against E. coli, as well as anti-inflammatory and antioxidant properties. Furthermore, histopathological examination of the intestinal broiler received BBR showed improvements and restore tissue near to normal.
Key...
Afsheen Khattak1, Shahida Naveed1*, Naila Khalid1, Inayat Ullah Khan2 and Tamana Bakht3
...wheat seeds to check the inhibitory and stimulatory effect, respectively. Whole plant of V. encelioides was collected, dried, powdered, and aqueous extracts were prepared at room temperature. Randomized Complete Design (RCD) with four treatments and one control were used in the experiment. Data was recorded for seed germination rate (%), shoot length (cm), radical length (cm), fresh biomass weight (g), dry weight (g), and moisture content (%) of wheat and maiz...
Hafsa Saeed, Soumble Zulfiqar, Abeedha Tu-Allah Khan and Abdul Rauf Shakoori*
... znt operon. The minimum inhibitory concentration of zinc, lead and cadmium were 10, 10 and 4 mM, respectively against K. pneumoniae KW. The strain also exhibited metal uptake and storage ability. Having amplified and cloned zntR gene, expressed and purified ZntR protein showed binding with zntA promoter. Increased transcripts of zntR in the presence of metal ions revealed its metal inducible nature. The molecular dynamics simulation and protein-metal ion dock...
Khansa Jamil1, Muhammad Ramzan Khan1, Asad Jan2 and Ghulam Muhammad Ali1*
...s and P. aeruginous. The inhibitory effect was compared to standard gentamycin. Four bioactive compounds were subjected to molecular docking from which all targeted compounds received scores ranging from (−3.5 to −5.9) kcal/mol with targeted protein. Hence the study revealed that bioactive compounds derived from the Caralluma tuberculata plant pose highly antibacterial activity and might be used to synthesize the antibacterial drug....
Rabiea Pervaiz, Shaista Bano*, Sarfraz Ali Tunio, Abdul Nabi Jatt and Aisha Amber Soomro
... The present study shows inhibitory spectrum of B. pseudomycoides SB13m with conclusion that Bacillus species from a diverse environment and Proteus mirabilis from clinical environment were strongly inhibited....
Mahdyl , A.M.M.; Hafezl , M.A.; EL-Dougdoug2, Kh. A.; Fawzyl , R.N. and Shahwanl , Eman S.M.•
...justify;">To explore the inhibitory effect of two biotic inducers on tomato plant against Cucumber mosaic cucumovirus (CMV), salicylic acid (SA) activities were determined in the inoculated and non-inoculated tomato plants. Identify of CMV was confirmed by some differential hosts and dot blot immunoassay (DBIA) using polyclonal antibody specific CMV. Water extracts of Mirabilis jalapa, Clerodendrum inerme and Kombucha as antiviral to control the virus infectio...
El.-Kadyl, M.A.S; Badr2, A.B.; zein3, Saiwa N. and Khalifa4, M.A.A.
.... Ribavirin had a higher inhibitory effect (80%) when sprayed at lower concentration (the minimum inhibiting concentration is 0.0001%) before one hr of inoculation. Salicylic acid and Parahydroxy benzoic acid has the same effect (80% reduction) at concentration of 0.01% and 0.1, respectively in the treatments inoculated one hr. after sprays. Six different chemical compounds applied as foliar sprays with two concentrations for each were used to study their effe...
E.F. Mohamedl and A.A. Owayss2
Rizqan*, Hilda Susanty, Linda Suhartati, Azhar, Arief, Elly Roza
...roperties as well as its inhibitory effects against Staphylococcus aureus and Escherichia coli pathogens. Eco-enzyme was prepared from citrus peel waste, resulting in a solution with a typical fresh sour aroma. The pH of the eco-enzyme ranged from 3.7 to 4.5. Conducting the agar diffusion test revealed a dose-dependent inhibitory effect of the citrus fruit peel eco-enzyme against both Staphylococcus aureus and Escherichia co...
Ahmed M. Manthoor, Ali H. Saliem*
...e of inhibition, minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), by using 4, 8, 16, 32, 64 and 128 mg/ml concentration of the extract, while 1.562, 3.125, 6.25, 12.5, 25, 50, 100, 200, 400, 800 µg/ml concentration of Ciprofloxacin, on the other hand study mechanism of action of the extract against the same bacteria by Fe-Scanning Electron Microscopic(Fe-SEM). The extraction ratio was (15%) and the phytochemical an...
Tabinda Nowsheen1, Sayed Wadood Ali Shah2, Ali Hazrat1*, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar4 and Muhammad Ajmal Khan3
... of proteins. In minimum inhibitory concentration assay, the crude methanolic extracts showed significant inhibition against all tested bacterial strains at25, 50 and 100 µg/ml. The methanolic crude extract of A. maritime various parts showed MIC of 37.5µg/ml for S. aureus which is gram-positive bacteria followed by 75µg/ml for P. aeruginosa, (gram negative), and B. subtilis (gram positive) that is nearly similar to the activity of ciprofloxa...
Aalaa S. Saad1*, Taghreed H. A. Ali2, Nayerah Alatfeehy3, Dalia Elmasry4
...tion. PE’s minimum inhibitory concentration (MIC) was 50 and 25 mg/mL, while PN’s was 150, 75, and 37.5 mg/mL. The number of viable L. monocytogenes in the breaded chicken panne with PE and PNs gradually decreased significantly (P < 0.05) on chilling compared to the control. Moreover, in the PE and PN-treated groups, the relative expression of hemolysin A (hlyA) decreased until the 9th day, reaching 0.251 and 0.125, respectively, and the re...
Pramudya Andiana*, Moch. Geerhan Miraja Syahdan, Khothibul Umam Al Awwaly, Abdul Manab
...ity, and α-amylase inhibitory activity. CHP1 showed the best bioactivity with a DPPH IC50 value of 4.62 mg/ml, iron chelating ability of 81.07%, copper chelating ability of 35.97%, and α-amylase inhibitory activity of 32.67%. While the best reducing power was at CHP2. As a result, it can be concluded that the use of a combination of bromelain and papain enzymes in chicken head protein hydrolysate has the potentia...
Muhammad Akram1, Anirban Mandal2*, Mehwish Iqbal3, Arindam Mukherjee4, Ritika Bandyopadhyay5, Surendar Rangasamy6, Rida Zainab1, Muhammad Talha Khalil1, Pragnesh Parmar7 and Umme Laila1
SYED MUJTABA HAIDER1, KHIZAR HAYAT BHATTI1*, EJAZ HUSSAIN SIDDIQI1, SYED MAZHAR IRFAN1,`2, & ALLAH BAKHSH GULSHAN2
...t showed relatively more inhibitory effects on bacterial growth as compared to other solvent extracts....
HANNANA MARYAM, SANA MAQSOOD & SIKANDER ALI*
...ious techniques. Minimum inhibitory concentration (MIC) and critical dilution assays (CDAs) were performed for exhibiting bacteriocin inhibitory potential against various indicator strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Azotobacter sp. It was concluded that Brevibacillus sp. possesses strong potential for laterosporulin biosynthesis. Further manipulation of physical and nutritio...
MUHAMMAD NADEEM1, MUHAMMAD WASEEM MUMTAZ1&MUHAMMAD DANISH1
...based on α-amylase inhibitory assay (IC50=42.91±1.24 μg/mL) was also depicted to be highest in the same extract. Based on current findings, 60% ethanolic extract of V. negundo leaves was revealed to be most potent for its antioxidant and α-amylase inhibition activity, hence could be used for formulation of new plant based functional food sornutraceuticals to improve human health....
Youfang Li1, Xu Li2, Shaik Althaf Hussain3, Jayasimha Rayalu Daddam4 and Zhigang Chen5*
...hough, EGCG has a potent inhibitory effect on urinary stone formation, and the antioxidative action of EGCG is considered to be involved. The combinational activity was enhanced along with quercetin....
Tianhoun Denté Fidèle1,2*, Meda Nãg-Tiéro Roland2, Zabré Geneviève3, Koama Benjamin2, Kaboré Adama1, Tamboura H. Hamidou1, Bélem Adrien Marie Gaston4
...se-dependent manner. The inhibitory concentration 50 (IC50) for egg hatching ranged from 2.189 and 2.994 mg/ml for the hydroacetone and aqueous extracts respectively, and that for larval development was 1.970 for the hydroacetone extract and 2.761 mg/ml for the aqueous extract. The addition of polyvinylpolypyrrolidone (PVPP) to the extracts showed that the tannins present were responsible for the bioactivity observed in both plant extracts. These results show ...
Shah Zeb Khan1, Ali Hazrat1*, Farman Ali Khan2, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar3, Muhammad Ajmal Khan4 and Hayat Ullah1
...strong DPPH free radical inhibitory effects. Strong antioxidant activity was found for BR (IC50= 62.5 µg/mL) and BS (IC50= 80 µg/mL) probably due to the presence of flavonoids in butanol fractions, evident from the Fourier Transform (FT-IR) spectral analysis which gave distinct absorption of flavonoids functionalities. In antimicrobial studies, CR exhibited strongest antibacterial activity against E. coli, S. flexneri and S. aureus with inhibition ...
Nimra Javaid1, Mukhtar Hussain Shah1, Iqra Haider Khan2, *Arshad Javaid2 and Syed Muhammad Waleed1
...xtracts were also highly inhibitory in suppressing the germination up to 89% and 95%, shoot length up to 80% and 89%, root length up to 86% and 91%, and plant biomass up to 89% and 98%, respectively. Stem extract showed the least herbicidal activity causing up to 46%, 59%, 73% and 37% reduction in germination, shoot length, root length and plant biomass, respectively. This study concludes that leaf, root and inflorescence extracts of A. conyzoides possess stro...
Mona Adel El-Wakeel1 and Ibrahim Mohamed El-Metwally1
...ded results revealed the inhibitory allelopathic effects of orange peels powder
on both weeds with direct relationship between the orange peels rate and it's inhibitory
effects. However, the pre-sowing treatments were more effective than the others which
applied at the time of sowing. Orange peels at 40g/pot at one week pre-sowing gave the
highest inhib...
Muhammad Ather Nadeem1, Bilal Ahmad Khan*1, Sadia Afzal2, Ahsan Aziz1,
Rizwan Maqbool3, Muhammad Mohsin Amin1, Amir Aziz4, Amjed Ali1, Muhammad
Adnan1, Durrishahwar5
...li. The aqueous extracts inhibitory effect was in order of
leaf > fruit > stem. This study leads to conclusion that 8% or higher concentration of C.
tinctorius leaf aqueous extract may be exploited to biologically suppress the E. cruss-galli.
...
Muhammad Ather Nadeem1 , Bilal Ahmad Khan*1, Saima Anwar2, Ahsan Aziz1, Rizwan Maqbool3, Muhammad Kashif Sohail4, Muhammad Ehsan Safdar1, Muhammad Mansoor Javaid1, Muhammad Mohsin Amin1, Muhammad Kashif Iqbal1, Amir Aziz4
...acts showed stimulatory, inhibitory and hormetic responses depending
upon the concentration and the plant part used for extraction. Results depicted maximum
mean emergence time (5.26 days) and minimum germination index (1.67), germination
percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of red rice with
application of 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S.
Iqtidar Hussain1, Ejaz Ahmed Khan1, Jawad Nazir and Ehtesham-ul-Haq1
...edarachshowed pronounced inhibitory effect on all parameters of T. aestivum. M. azedarach exerted phytotoxic influence on T. aestivum at initial growth stages. M.azedarachexhibited a significant negative impact on germination of T. aestivumat100, 200, 300, 400 glitterof leaves than control (sterilized soil).M. azedarachhalted the coleoptilelength of T. aestivum@ 400 gleaves litter-1 (T4). Powdered leavesof M. azedarachin clay loam soil appeared to have strong ...
Nabeel Akhtar1, Owais Iqbal1,2*, Imtiaz Ahmed Nizamani1, Rehana Naz Syed1 and Abdul Mubeen Lodhi1
...monstrated an escalating inhibitory trend as the concentration increased further....
Somayeh Amani,Mehrdad Chaeechi,Mohsen Rajabi
SHUMAILA FAROOQ,MAKSHOOF ATHAR,Salik Nawaz Khan,SUMERA NAZ
Wirat Phuwiwat , Watcharin Wichittrakarn , Chamroon Laosinwattana , Montinee Teerarak
Mohammad Zubair Sulemani* Mohammad Safdar Baloch** and Khalid Abdullah**
...s conducted to study the inhibitory effects of leaf and litter leachates (extracts) of kikar (Acacia nilotica) on the germination of wheat (Triticum aestivum) maize (Zea mays), sorghum (Sorghum bicolor), millet (Pennisetum typhodeum), sunflower (Helianthus annus), brassica (Brassica compestris) and taramira (Eruca sativa). Seed germination of all sevel crops was significantly and negatively affected due t...
Fauzia Balqis Malik and Bashir Hussain Shah
... There is no significant inhibitory effect due to allelochemicals on various growth parameters including yield (weight and number of pods) of Phaseolus vulgaris. However, the growth of Pisum sativum was significantly reduced due to allelopathic effect of all the three Eucalyptus species, maximum being of the decayed leaves of Eucalyptus citriodora followed by Eucalyptus camaldulensis and Eucalyptus tereticornis respectively. Similarly nodulation ...
Nasim Akhtar, Himayat Husssain Naqvi and Farrukh Hussain
...ances which proved to be inhibitory not only to its own growth but also to other species used in the bioassays. The toxicity in both the cases increased with increasing concentration and soaking time. Cinhrus had more allopathic potentiality than Chrysopogon. The toxicity in both the cases was species slated. The possible effect of allelopathy of these grasses is discussed in relation to the vegetation dynamics....
