Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*
...y of two techniques i.e. serum neutralization test (SNT) and solid-phase competitive ELISA (SPCE) for foot and mouth disease virus (FMDV) structural antibodies detection in terms of their sensitivity and specificity. These methods were performed by using set of sera collected from cattle with foot-and-mouth disease vaccination. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years...
John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1
...">< 100% human serum or a tick homogenate, the fluorescent aptamer assay maintained sensitive detection of Rickettsia typhi cells. ...
Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren
...imation of antibodies in serum and dried blood samples collected from mink, a VP2 ELISA (enzyme-linked immunosorbent assay) system was recently evaluated. However, such estimations of disease progression are commonly based on single sampling occasions and it is essential to know if estimated antibody levels vary over a period of weeks or months. Therefore, the aim of this study was to evaluate the short term stability and the long term ...
Ahmed Zein Mahmoud1, Muaz Abdellatif 2, 3*, Luai Shazali1
Muhammad Mushtaq, Umer Sadique, Naila Chand, Iftikhar Ahmed, Said Sajjad Ali Shah, Ijaz Ahmad, Imran Ullah and Muqadar Shah
...lysis. Lipid profile and serum glucose was significantly improved. Lipid profile and serum glucose was significantly reduced in all treated groups. Mean cholesterol was (157.66 mg/dl), triglyceride (65.64 mg/dl), HDL (58.88 mg/dl) while no alteration in LDL contents. Significantly effect of water based infusion was notice on mean serum glucose (73.83 mg/dl) Observation of the study indicat...
Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure
...tion in animal and human serum samples will utilize Microscopic Agglutination Test (MAT). Study outcomes will include (i) geo-referencing of adverse environmental attributes that support Leptospira growth, (ii) animal and human seasonal public health burden of Leptospirosis, (iii) seasonal variation in the distribution of Leptospira determinants in a slum setting, and (iv) a simulation model based on an ecological metapopulation framework to track Leptospira b...
Tasnim Farasat*, Saima Sharif, Farkhanda Manzoor, Muneeza Zafar and Shagufta Naz
...e, cholesterol level and serum insulin were significantly higher (p< 0.05) in proliferative group of diabetic retinopathy, while triglyceride level, HbA1c (%), and low density lipoprotein (LDL) were non-significantly higher (p> 0.05) in diabetic retinopathy groups. To conclude high prevalence of diabetic retinopathy was observed among newly diagnosed diabetic patients....
Abdul Sattar Baloch1*, Abdul Rasheed1,2, Rahmatullah Rind1, Jam Kashif Sahito1, Rehana Buriro1, Muhammad Faisal Ayoob1 and Parkash Dewani1
...engal Plate Test (RBPT), serum agglutination test (SAT) and competitive-ELISA (c-ELISA) were used as screening tests, and overall seroprevalence of brucellosis was found to be as 21%, 21% and 13% by RBPT, SAT and c-ELISA tests, respectively. Seroprevalence was higher in females (26%) than in males (16%) by RBPT and SAT tests, and also in camels less than 9 years of age (13.33%). Milk samples were collected to detect the antibodies against the disease, however,...
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*
...ibet, China. A total 454 serum samples were collected from Tibetan pigs in Nyingchi in 2014 and 2015. Each sample was assayed for T. gondii antibodies by a commercial enzyme-linked immunosorbent assay kits and an indirect hemagglutination test. The results showed that seroprevalence in Tibetan pigs were 25.6% by ELISA. On gender basis, the prevalence was found 25.1% in males and 27.8% in females. By the method of IHA, the seroprevalence in Tibetan pigs were 21...
Hussain Ahmad1, Inamullah1, Ijaz Ali2, Tauseef Ahmad3, Muhammad Tufail4, Kabir Ahmad5 and Bibi Nazia Murtaza3,6*
Asmaa Hamid1,* and Samia Kalsoom2
...were monitored for total serum cholesterol. It was found that both Bengal gram and chickpea had a significant effect on lowering glucose level and total cholesterol of these experimental animals. Bengal gram however, proved to have a significantly (p<0.05) greater effect on lowering of these parameters....
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
...lification strategy. The serum samples were analyzed for determination of metals and minerals through Atomic absorption spectrophotometer (AA 6600 Shimadzu). The statistical analysis indicated that non-significant association existed between SOD1 (rs2070424) gene polymorphism and RA (p>0.05). Results of HWE estimation indicate that allele frequencies were not deviant from HWE in RA group. The results of present study indicates that Pb and Cr concentration d...
Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar
...Pakistan. A total of 965 serum samples were collected from sheep and goat’s farms from January to December 2015 with no history of vaccination. Haemagglutination inhibition (HI) test was performed using PPRV antigen to determine antibody titres level. The overall prevalence of antibodies to PPRV in goats and sheep were 66.22% (521 samples) and 65.76% (444 samples), respectively. Sero-prevalence of anti-PPRV antibodies was found higher in adults (69.14% w...
Wan-Long Zhu1 and Guang Yang2,*
... body mass, food intake, serum leptin levels and hypothalamic neuropeptide neuropeptide Y (NPY), Agouti related peptide (AgRP), pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) expressions were measured. The results showed that short photoperiod reduced body mass and body fat mass, and increased food intake. But serum leptin levels showed no significant differences between short photoperiod gr...
Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki
... cycle in Nigeria, 3,288 serum samples were collected from domestic pigs from 10 states and analysed for anti-tick antibodies using the recombinant TSGP1 indirect ELISA. Of the samples analysed, 13.4% (442/3,288) showed moderate to high reactivity, suggestive of domestic pigs’ exposure to tick-bite. Antibody reactivity’s were found in eight of the 10 states studied and all the states have favourable climatic conditions for soft ticks’ surviva...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...he study. Blood profile, serum biochemistry and mineral profile were analyzed at day 0, 7 and 21 of infection. Results showed significant decrease (P<0.05) in red blood cells (RBC), packed cell volume (PCV), hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC) and platelets while total leucocytes count (TLC), neutrophils, eosinophils, basophils, lymphocytes, monocytes, red cell distribution width (RDW) and mean corpuscular volume (MCV) were inc...
Shazia Ali, Hizb Ullah and Sarwat Jahan*
...uced (P<0.001), while serum ferritin were significantly increased (P<0.001) in all four thalassemic groups on comparison with control. Kisspeptin levels were significantly reduced in ≤13 years female (P<0.001). While significantly high levels were observed in >13 years of male (P<0.01). T3 levels were significantly increased in both thalassemic groups of female (P<0.01). While T4 levels were significantly reduced in &...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...rs5888 polymorphism with serum lipid levels and CVD risk in an Indian population. A total of 412 samples which included 148 myocardial infarction survivors, 162 Normolipidemic healthy controls and 102 patients with hypercholesterolemia in a Indian Tamilian population were included in the study. Genotyping of SCARB1 genetic polymorphisms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found t...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2*
...province, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of avian leukosis virus subgroup A (ALV-A) infection was confirmed by necropsy, histopathological examinations and PCR analysis. The results showed, 20 chickens as positive for ALV-A (2.73%, 95% CI 1.7-4.2) and these chickens were found to be positive for ALV-A with the further distribution of 2.91% (95% CI 1.0-6.7), 3.70% (95...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3
...and IgM were detected in serum by ELISA method. Toxoplasma gondii DNA was detected using PCR. Most women were in the 20-29 years age group (48%), followed by 30-39 years (33%) and >40 years (14%). Overall prevalence of Toxoplasma gondii IgG was seen in 12%, with significantly more in women with age more than 30 years as compared to those with age less than 30 years (19% vs 8%; p=0.038). The percentage of IgG seroprevalence was higher in...
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
... Pakistan. A total of399 serum samples from indeginous and exotic cattle were tested for brucellosis by RBPT, SAT and multiplex real-time PCR for Brucella sp., Brucella abortus and Brucella melitensis. A total of 20 (5.01%) samples were found seropositive by RBPT and 19 (4.76%) by SAT, however real-time PCR showed 13 (3.25%) samples positive for both Brucella (sp.) and Brucella abortus. None of the seropositive samples were f...
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...Pakistan. A total of 120 serum samples were randomly collected from buffaloes and cattle (60 per species) at Civil Veterinary Hospitals, animal markets and peri-urban livestock holdings in Rawalpindi and Islamabad. Serum samples were initially screened by the Rose Bengal Plate Test (RBPT). RBPT positive samples were subjected to a B. abortus specific indirect enzyme-linked immunosorbent assay (i-ELISA).
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
... supplemented with horse serum. The blood was used for serum plate agglutination assay. Thereafter, the samples were subjected to genomic DNA extraction to detect Mycoplasma spp. using multiplex-PCR. Isolation and identification data showed that a total of 58/96 samples (60.41%) were positive for Mycoplasma infections whereas a total of 61/96 blood samples were also found positive using serum<...
Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3
...of biochemistry markers (serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP)). In contrast, in the groups given D-GaIN and EA, a decrease in the damage of the liver tissue, a significant decrease activated Bax and caspase-3-positive hepatocytes, while increase in the number of Bcl-2 positive hepatocytes, a decrease in the biochemistry markers levels were found. Group 4, given EA before D-GaIN, showed better results whe...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...ic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
...
Physiological Responses to Fasting and Refeeding in Apodemus chevrieri from Hengduan Mountain Region
Hai-Ji Zhang1, Di Zhang2, Dong-Min Hou1 and Wan-Long Zhu1,*
...RMR), organs morphology, serum leptin levels and food intake were measured in the present study. The results showed that food deprivation decreased body mass and body fat mass. After refeeding, body mass can not be returned to the control value on refeeding 12 h, and it returned to control level on refeeding 7 days, but body fat mass can not be restored to the control level on refeeding 7 days. RMR and mass of liver decreased significantly in fasting groups, w...
Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
...y, the hematological and serum biochemical parameters were determined to establish the reference values in the wild Indian blue peafowl (Pavo cristatus) of Thar (desert) region in the Sindh province of Pakistan. A total of 60 blood samples were collected from peafowl (30 males and 30 female) in months of March and April, 2015. The gender difference showed that RBC, MCV and MCH concentrations were significantly higher (P < 0.05) and the
Mutassim M. Abdelrahman1*,Ibrahim Alhidary1, Abdullah H. Alyemni2, Rifat U. Khan3, Abdel Raouf S. Bello4, Mohamed Y. Al-Saiady2 and Ramzi A. Amran1
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...ent assay (ELISA) in all serum samples. This study indicated that the frequency of both alleles HLA-DRB1*11and HLA-DRB1*12 in aero allergy patients is less as compared to healthy controls. HLA-DRB1*11 demonstrated significant association with aeroallergens and could have a protective role for allergy while HLA-DRB1*12 did not show any association with aeroallergen sensitization.
...
Farah Ashfaq* and Tasnim Farasat
...mine the relationship of serum resistin with metabolic markers in obese Pakistani subjects. Anthropometric parameters including age, body mass index (BMI), waist circumference, waist-hip ratio (WHR), diastolic and systolic blood pressure, lipid profile and fasting glucose, serum resistin and insulin of three hundred over weight, obese males and females, 17 to 30 years and 100 comparable control subjects were included.
Shabana1,*, Farah Ehsan1 and Shahida Hasnain1,2
...t its effect by changing serum fasting plasma glucose, total cholesterol and LDLC levels.
...
Ambreen Butt1, Uzma Malik2, Khadija Waheed3, Amna Khanum3, Samar Firdous4, Sara Ejaz5 and Fawad Randhawa2, Tania Shakoori6*
...
Research shows that serum Vitamin B12 levels are reduced in diabetes including gestational diabetes. We sought to compare serum cobalamin between pregnant females diagnosed with gestational diabetes mellitus (GDM) and healthy controls and to propose a cut off value for serum B12 levels as a predictive biomarker for GDM. Study design was Quantitative cross-sectional study. This study w...
Zia-ur-Rehaman1, Naila Chand1, Sarzamin Khan1 and Rifat Ullah Khan2,*
... from day 21 to 42. Mean serum antibody titer against Newcastle disease (ND) and serum paraoxonase (PON1) were significantly higher (P< 0.05) in TN zone, while serum malondialdehyde (MDA) was significantly (P< 0.05) higher in HAT zone. No significant differences were recorded in serum antibody titer against ND, PON1 and MDA among different broiler ...
Rehana Shahida1, Tasnim Farasat1, Shagufta Naz1,* and Shahjahan2
...ng plasma glucose level, serum levels of insulin, IL-6, and TNF-α. Serum level of thrombomodulin was assessed as a measure of vascular damage. Different demographic parameters as age, BMI, waist /hip ratio, B.P., personal history and socioeconomic status were recorded. Fasting glucose and HbA1c was done by chemistry analyzer. Fasting serum insulin, IL-6, TNF-α and thrombomoduli...
Jiandong Wu, Miao Ye and Zaigui Wang*
... reducing the content of serum total cholesterol (TC), triglycerides (TG), and low-density lipoprotein-cholesterol (LDL-C) and increasing the concentration of high-density lipoprotein-cholesterol (HDL-C).
...
Tahira Perveen1,*, Shaista Emad1, Saara Ahmad2, Zehra Batool1, Sarwat Yousuf1, Sheeza Sheikh1, Sara Qadeer1 and Saida Haider1
Irfan1* , Arshad Javid2 , Muhammad Altaf3 , Muhammad Shahbaz3 , and Khalid Javed Iqbal4
...an";">Variations in serum biochemical profile with increase in age were analyzed in turkeys (Meleagris gallopavo). Genderwise variations and effect of rearing systems i.e. free range, semi-intensive and confinements were also assessed from 1st to 6th month of age. Total sixty (n = 60) experimental birds were divided in to three groups i.e., poults having age 4-8 weeks (10 &...
Muhammad Iqbal Anjum*, Shahbaz Javaid and Mukhtar Ahmad Nadeem
... and 7.11 mg/dL in blood serum and 32.61, 31.36 and 32.83% and 16.57, 15.92 and 16.60% in tibia bone of chicks fed rations I, II and III, respectively. The total feed cost per unit weight gain of broilers fed R-III diet was numerically 14% and 9% less than the broilers fed R-II and R-I, respectively. Results suggested that exogenous microbial phytase supplementation to rations having low DCP had positive effects on weight gain, feed: gain ratio and economic ef...
Rabia Qurashi, Qurat ul Ane Gillani* and Furhan Iqbal*
...ys, on hematological and serum biochemical profile of 6 week old male albino mice. Blood samples from male albino mice (CGP55845 treated N = 14 and saline treated N = 16) were collected directly by cardiac puncture and used for estimation of hematological and some serum biochemical parameters. The hematological parameters did not show any significant variation between the two groups. From amongst ser...
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
...extracts (150 mg/kg) and serum samples were collected from overnight fasted diabetic rats on day 15 to observe thepercent changes in blood glucose levels and lipid profile. Garlic extracts have shown the significant (p<0.001) hypoglycemic activity with no considerable effect on mean body weight of rats (ns, p>0.05) as compared to diabetic rats. The AGE treatment has significantly reduced the blood glucose level (56%), however, the EGE treatment was found...
Muhammad Usman Saleem1, Saima Masood1,*, Hafsa Zaneb1, Aneela Zameer Durrani2, Asim Aslam3, Kamran Ashraf4, Habib-ur-Rehman5, Muti-ur-Rehman3 and Muhammad Shabir Shaheen6
...al microarchitecture and serum biochemistry in Hubbard chicks. A total of 128 one day-old chicks were divided equally in four different groups with each group having 4 replicates. The number of birds per group were 32 whereas the number of birds per replicate were 8.The first group was kept as control (CONT) whereas, the second (MOSG) and third (OABG) groups were given MOS (2g / kg of feed) and OAB (3g / kg of feed), respectively. The fourth group (MOS+OAB) wa...
Attia Anwar1, Sajida Malik2*, Zobia Usman1 and Sadia Chiragh3
...enzymes, renal function, serum uric acid and blood cell counts of Sprague-Dawley rats. For this purpose, twenty Sprague-Dawley rats were divided in two groups. Group A (n=10) was given distilled water and Group B (n=10) was supplemented berberine hydrochloride with dose of 75 mg/kg body weight/day. 24 weeks post-treatment, blood samples were collected by cardiac puncture from both groups and tested for Hb (%), WBC, RBC and platelet counts, SGPT, alkaline phosp...
Kashif Prince1,*, M. Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Jawaria Ali Khan1, Nisar Ahmad3,Rais Ahmed2, Aamerzish Mushtaq4, Sajid Umar4 and Yung-Fu Chang5
...esigned to determine the serum selenium status in association of its risk factors in cattle and buffaloes of district Kasur, Punjab. Selenium status was evaluated by Atomic Absorptions Spectrophotometery (AAS) with respect to sex of animals, geographical area, age of animals, herd size, stage of animals, production level and concentrate feeding. Selenium deficiency was also evaluated as a risk factor of mastitis and repeat breeding. About 48.43% cattle and 72....
Abida Sajid* and Aqsam Sajid
...ncy Anemia is defined as serum ferritin level less than 12 micro gram per ml and on Red Blood Cell indices, i.e. decrease in MCV, MCH and MCHC, and Microcytic Hypochromic cells in Peripheral Smear. Megaloblastic anemia was labeled when Macrocytic Hypochromic cells seen. Obstetric outcome in terms of Preterm delivery defined as delivery at <37 weeks of gestation (36 +6 weeks) and birth weight <2500g mand a need for operative delivery. Patients fulfilling ...
Muhammad Athar Khan1, Muhammad Zahid Latif2*, Syed Amir Gilani3 and Ifrah Bukhari4
...council as controls. The serum sample of each subject was tested against each of the five antigens against the serovars. A total of 250 subjects were included in the study. Out of these, 125 subjects were exposed to the rice paddy water where as 125 were not exposed to rice paddy water. The cumulative seropositivity among the exposed is (83.2%) as compared to (42%) among the non exposed to rice paddy field water. The calculated cumulative odds ratio is 6.7 whi...
Sana Qanber Abbasi1*, Ejaz Ahmed2 and Rabia Sattar1
...een found between rising serum pro BNP levels and Cardiovascular pathologies especially those involving left ventricular dysfunction which may depict a probable association between migraine and CVD. To compare the levels of serum pro Brain natriuretic peptide (pro BNP) between migraineurs and hypertensive migraineurs. It was a cross sectional comparative study in which serumpro BNP levels ...
Promy Virk
...d groups. The results on serum ALT, creatinine levels and blood urea also showed a comparable ameliorative effect of both the bulk Trigonella seed extract (TBN) and the green nanocomposite (TN), being more efficacious than metformin. Thus, the green synthesis and application of the Au/Ag nanoparticles offer a novel approach in nanomedicine for diabetes management.
...
Muhammad Kashif Maan1, Muhammad Arif Khan1, Shehla Gul Bokhari1, Muhammad Ijaz2, Hamid Akbar1, Sajid Umar 3 and Muhammad Luqman Sohail4,*
...terectomy.
...
Zhiping Hu, Hussain Ahmad, Jingfei Zhang, Lili Zhang and Tian Wang*
Shireen Ihsan Izzaddeen1 and Ali Kaygısız2,*
...;0.05). The calf’s serum total cholesterol, glucose, AST, GGT, creatinine, phosphor, calcium, insulin, total T3, total T4, albumin, GH and globulin concentrations did not differ between the groups (P>0.05). Essential laurel oil containing whole milk, on the other hand, lowered serum triglyceride concentration (P<0.05). Altogether these results suggested that the consumption of laurel essential oil (600 mg/day) co...
Lili Zhang, Yuanxiao Wang, Yili Kong, Hussain Ahmad, Rui Yan, Li Dong, Jingfei Zhang and Tian Wang*
...and 160 days of age, the serum urea nitrogen levels of the IUGR pigs were significantly higher than the NBW pigs (P<0.05). The serum insulin levels of the IUGR pigs were significantly lower (P<0.05) than the NBW pigs at 28 and 66 days of age. The serum leptin level was significantly higher (P<0.05) in the IUGR pigs at 28 days of age while it decreased significantly (P<0.05) at ...
Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4
...rs determined from blood serum were glucose, cortisol and Heat shock protein-70 (HSP-70). Thermal stress increased all physiological parameterssignificantly (P < 0.001). Holstein Frisian manifested maximumincrease in RT, RR and PR (3.33, 209.50 and 59.41%) than crossbred (0.59, 40.22 and 22.0%), Sahiwal (0.78, 42.54 and 34.31%) and Achai (0.78, 39.22 and 33.85%) respectively (P < 0.001).Thermal stress increased biochemical changes significantly (P < 0...
Ambreen Asghar, Tasleem Akhtar, Nadeem Sheikh*
...aluate the variations in serum protein level induced by fat rich diet. For this purpose, two groups of Wister rats were fed with diets carrying difference in percentage of fats. Protein profiling of treated groups indicated a marked increase in serum protein (related to iron metabolism and immune response) level compared to low fat-fed rats. Additionally, a decreased level of serum protein...
Naimat Ullah1,3,*, Aneela Zameer Durrani1, Muhammad Avais1, Nisar Ahmad2, Sana Ullah3, Muhammad Shuaib Khan3, Khalid Mehmood5, Mumtaz Ali Khan1 and Ikramul Haq1
...lanine aminotransferase, serum creatinine, urea whereas decrease in glucose level in diseased animals. The current study concluded that paying close attention to animal feeding, housing and keeping may reduce the occurrence of theileriosis in sheep.
...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...leukin-12 (IL-12) in the serum of ducks at 1 day and 7 days after LPS injection (P<0.05). However, there were no apparent changes in organ index or the relative expression of TLR4 and IgM (P>0.05). Furthermore, correlation analysis showed a positive relationship between the mRNA expression of TLR4 and concentrations of TNF-α and IL-12. These results revealed that although there were no significant effects of LPS injection on
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...servation. In total, 146 serum samples from 47 individuals were analyzed. Serum neutralization test was used to determine presence of CDV antibodies, and an indirect IgG ELISA was used to detect CPV antibodies. Neutralizing antibodies was induced after CDV vaccination and persisted up to 3.9 years. Most animals had high IgG antibody titers to CPV prior to vaccination and vaccination did not result in increased titers. A decl...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
... out of 507 samples, 479 serum samples were scrutinized by enzyme linked immunosorbent essay (ELISA). Sero-prevalence of AIV among different species of wild birds was as follows; peacock (n=35; 14%), duck (n=5; 2%), migratory water fowl (n=3; 1%), pheasant (n=2; 0.8%), grey leg goose (n=1; 0.4%), turkey (n=1; 0.4%), eagle (n=1; 0.4%) and crane (n=1; 0.4%), for domesticated bird species sero-prevalence was; broiler (n= 152; 60%), rural poultry (n=14; 6%), domes...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,*
... (AST) activities in the serum along with decrease in level of antioxidant enzymes and significantly increase in the MDA contents in TD afflicted chickens compared to control group. The SM administration to TD affected birds significantly ameliorated lameness, stimulated ALP level with a decrease in ALT and AST contents, increase in antioxidant parameter and decrease in MDA contents significantly (P<0.05). SM treatment of TD-afflicted birds prevented lamene...
Jia-kui Li1,2,*, Kun Li2, Zhao-qing Han2, Hui Zhang2, Xiao-qiang Wang2, Hou-qiang Luo2, Yan-fang Lan2 and Gang Qiu1,2
... China. Totally, 516 yak serum samples were randomly got during 2012 to 2014. BVDV was found highly prevalent among the adult yaks (51.1%; 95% CI: 46.5-55.6) and calves (60.5%; 95% CI: 43.4-76.0), respectively. The prevalence of BVDV infection in 2012 to 2014 was 45.3% (95% CI: 38.6%-52.1%); 56.0% (95% CI: 49.7-62.2) and 60.5% (95% CI: 43.4-76.0), respectively with a significant difference in the three years (P<0.05). The current result reveal a growing pre...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...ng proportion of chicken serum and some growth factors. CAFSCs can be transferred to 34 passages nowadays. Surface marker Oct4, CD105, nanog, CD73 and SSEA-4 on CAFSCs are detected positive with immunofluorescence histochemistry, Gene CD44, CD29, CD73 and SH2 are detected positive with RT-PCR. We carry karyotype analysis out and find that chromosome is 2n=78 and shows normal. CAFSCs in mid period of subculture propagate fastest from cell cycle examination on F...
Tayfun Karataş
...vation for 8 days on the serum metabolites and antioxidant enzymes, thiobarbituric acid reacting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss). Eight-days of fasting caused a significant decrease in glucose, total protein, triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein levels as well as protein and lipid reserves in liver and muscle tissue of fish (p<0.05). Th...
Muhammad Shahid Saeed1, Adeela Shahid2, Samia Jawed3, Muhammad Akram4, Irshad Hussain Qureshi5
...s taken to determine the serum magnesium levels. All patients were given Salbutamol inhalation and intravenous (I/V) Hydrocortisone. The study group was given 2gm of MgS04 intravenously in 20 minutes in a burette and Placebo group was given placebo (0.9% normal saline). Salbutamol inhalation was given at regular intervals and Spirometry was performed to determine lung functions.
Results: The two groups (study group and placebo-tre...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...ced significantly higher serum GH and PRL concentration in comparison with T2 and control groups (p < 0.05). Expectantly, mice in T1 group also showed significantly higher average weight gain and offspring weaning weight than that in T2 and control groups, respectively (p < 0.05). Overall, these results suggested that novel somatostatin plasmid fused to tPA signal peptide obtained stronger immunogenicity, and better potentials of pro...
Farhat Ijaz1, Rana Khurram Aftab2*, Samia Jawed3
...atients. TNF-α and serum insulin levels were determined using ELISA. Insulin resistance was calculated using HOMA-IR. Comparison between groups was performed using independent sample t-test. The P value ≤ 0.05 was considered statistically significant.
Results: Mean HOMA-IR and TNF-α values were significantly (p-value <0.01) higher in obese diabetics (17.13+8.77) and (10.96+4.69), respectively when compared to non-obese Type...
Muhammad Anwar Iqbal1, Habib Rehman2, Ali Hussain3,*, Faiza Jabeen4, Ijaz Ahmad2, Kamran Ashraf5, Muhammad Irshad Arshad6 and Omaima Khan7
... visceral organ weights, serum mineral levels and caecal concentrations of E. coli and Clostridium spp. in Japanese quail (Coturnix coturnix japonica). Japanese quail (one-day old; n = 1320) were taken and randomly separated into four groups (n = 320), having 8 replicates (n = 40) in each group. Corn-based poultry feed (basal diet) was given to control group birds (Group A) and the same feed mixed with three different concentrations of MOS...
Bo Wu1, Hui Zhang2, Kun Li2, Khalid Mehmood2,7, Yan Zhao1, Bin Jiang1, Chengjun Xue3, Muhammad Tariq Javed6, Fazul Nabi2, Zhaoqing Han5,* and Houqiang Luo4,*
...Zhejiang. A total of 368 serum samples were collected from June to July in 2016. Out of these samples, 23 (6.79%, 95% CI 4.4-9.9) pigs were found positive for FMD antibodies with the further distribution of 9.09% (95% CI 4.6–15.7), 6.90% (95% CI 2.6–14.4), 5.49%, (95% CI 1.8-12.4) and 4.35% (95% CI 0.9-12.2) from Wenzhou, Lishui, Jinhua and Ningbo counties, respectively. For the first time, we investigated the seroprevalence and the immunization sc...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
...h and mobility of bovine serum albumin (BSA) was different in all milk species.
...
Shoukat Ara1, Sheikh Adil2,*and Manzoor Ahmad Khan3
... cristata on growth, serum biochemistry and laying performance layer chicken, a study was conducted on 240 key stone golden layer chicks which were randomly assigned into five groups having three replicates of 16 each. Birds in control (T1) group were fed the basal diet, where as other groups the groundnut cake was replaced with 5 (T2), 10 (T3), 15 (T4) and 20% (T5) Azolla. The results revealed ...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
...changes were observed in serum biochemistry and histology. Serum glucose and cholesterol were increased while total protein was decreased. Histopathological result reveled that gills of experimental fish was damage severely resulting Necrosis, Damaged nuclei , rupturing of epithelial cells, Mucous cells and severe lamellar fusion. It was concluded that, deltamethrine is highly toxic for non-target aquatic organisms like silv...
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
... testing the contents of serum level of tumour necrosis factor-α (TNF-a), interleukin-6 (IL-6), lipopolysaccharide (LPS) as well as colonic mucosal tissue TLR4/9 and NF-kB. We got the following results: (1) The rat IBS-D model was successfully prepared; (2) Wumei Pill could reduce the contents of serum TNF-α, IL-6 and LPS in rats(P<0.05), and alleviate the inflammatory reaction and accumulation of bacterial en...
Qudrat Ullah1,*, Huma Jamil1, Zafar Iqbal Qureshi1, Muhammad Saqib2 and Heinrich Neubauer3
...ii antibodies in the serum. Serological analysis revealed overall flock-level sero-prevalence of 92.3% which varied from 88.88% in sheep and 100% in goat, while individual level sero-prevalence was 15.6% in sheep and 15.0% in goats, the difference being non-significant. A significant (P<0.05) association was found between seropositivity against C. burnetii and variables like livestock farm, type of farming, region (locality of farm), prese...
Bata Shalangwa Ishaku1*, Maimadu Abdullahi1, Dakwang Nalong1, Rengkat Jonah1 and Olabode Mayowa2
...eria. Five hundred (500) serum samples comprising of 300 pigs, 100 each of sheep and goats were collected and analyzed for Toxoplasma gondii antibodies (IgG) using latex agglutination test (LAT). Serum samples with LAT titer >10 IU/ml were considered positive. The study showed that 176 of the 500 samples analyzed were positive for Toxoplasma gondii antibodies (IgG) giving an overall prevalence of 35.2%. There was statisti...
Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq2
Muhammad Babar Khawar, Nadeem Sheikh*
Effect of paper industry leachate on various serological indices and serum proteins of wistar rats
...e variations in level of serum aspartate aminotransferases (AST) (P<0.0001), cholesterol (P<0.0001) and High density lipoproteins (HDL) (P<0.0001) while a significant negative change in triglycerides (P=0.0002) and creatinine (P=0.0370) level in both experimental groups. Alanine aminotransferases (ALT) level showed a significant increment in Group 1 and a decrement in Group 2 compared to control group (P<0.0001). SDS page analysis revealed an overa...
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
Riffat Mehboob1*, Sami Ullah Mumtaz2, Zoya Manzoor3, Sajid Abaidullah2, Fridoon Jawad Ahmad1
Deranged biochemical and hematological profile of septicemia patients in Mayo hospital, Lahore
...ish the possible role of serum biomarkers of liver, kidney and blood in the diagnosis of septicemia. 101 confirmed patients of septicemia from a tertiary care hospital in Lahore were included. Liver and renal function tests were performed for all patients. Patients were divided into 3 age groups on the basis of age: 30-50, 51-70 and 71-90 years. In Liver function Tests, ALT (37.62%), AST (50.49%) and ALP (99%) were elevated, Bilirubin was normal in majority of...
Nasir Raza Zaidi1, Mian Waheed Ahmad1, Mahesh Gautam1, Riffat Mehboob2*
Risk factors for multiple sclerosis in Pakistani population- A crosssectional study
... had low level of
serum vitamin D and 30% of the patients had positive family history. Current study shows viral infections, smoking,
and hypovitaminosis D as frequently associated with MS....
serum vitamin D and 30% of the patients had positive family history. Current study shows viral infections, smoking,
and hypovitaminosis D as frequently associated with MS....
Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*
Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
..., as noted by changes in serum proteins and transaminases. Therefore,
further investigation of its toxicity is necessary in order to consider it safe....
further investigation of its toxicity is necessary in order to consider it safe....
Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1
Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
...te changes in tissue and serum total proteins and
albumin along with urea, uric acid and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume ...
albumin along with urea, uric acid and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume ...
Nabila Roohi, Mehjabeen, Samina Ashraf*
Effects of cigarette smoking on serum proteins profile in male active and passive smokers
...als with the analysis of serum total proteins and fractions
(albumin, total globulins, gamma globulins and non-gamma globulins) of active and
passive smokers. Blood samples of 180 cigarette smokers were collected from
different locations of Lahore and 60 healthy non-smokers from University of the
Punjab, Quaid-e-Azam campus Lahore, in terms of comparable age, height, weight
and socioeconomic set up. S...
(albumin, total globulins, gamma globulins and non-gamma globulins) of active and
passive smokers. Blood samples of 180 cigarette smokers were collected from
different locations of Lahore and 60 healthy non-smokers from University of the
Punjab, Quaid-e-Azam campus Lahore, in terms of comparable age, height, weight
and socioeconomic set up. S...
Anber Naqvi1*#, Abdul Qadir1, Adeel Mahmood2, Mehvish Mumtaz3, Iqra Aslam1, Gan Zhang4
Assessment of polychlorinated biphenyls (PCBs) in maternal blood serum from selected districts of Punjab, Pakistan
...he PCB residues in blood serum of
mothers residing in five districts of Punjab Province, Pakistan. The mean
concentration of Σ34 PCB congeners was recorded 232.3 ng/g l.w. PCB homologs
profile showed highest levels of Tetra-CBs (53%) followed by Penta-CBs (23%)
and Hexa-CBs(11%), respectively. Spatial distribution of PCB homolog reflected
the higher prevalence of lower chlorinated PCBs in urban population descending
mothers residing in five districts of Punjab Province, Pakistan. The mean
concentration of Σ34 PCB congeners was recorded 232.3 ng/g l.w. PCB homologs
profile showed highest levels of Tetra-CBs (53%) followed by Penta-CBs (23%)
and Hexa-CBs(11%), respectively. Spatial distribution of PCB homolog reflected
the higher prevalence of lower chlorinated PCBs in urban population descending
Hina Abrar1,2, S.N.H. Naqvi1,*, Muhammad Rashid Ahmed3, Asma Basharat Ali3 and Hina Yasin1,2
A.M. Abdul Azeem, A.N. El Shahat* and Ashraf M. Mounir
...significant reduction in serum activities of liver enzymes, level of hepatic and testicular lipid peroxidation and plasma levels of caspase 3 associated with significant increase in the levels of luteinizing hormone, follicle stimulating hormone and testosterone in addition to marked improvement in the level of total antioxidant capacity and glutathione content and the activity of superoxide dismutase activity and catalase activity of the liver and testes of l...
Muhammad Ijaz1, Shahid Hussain Farooqi1, Rahmatullah2, Amjad Islam Aqib1, Sadaqat Ali3,*, Awais Ghaffar1, Ahmad Ali1 and Sehrish Saleem1
...n hematocrit and various serum biochemical parameters (serum electrolytes, trace and ultra-trace elements) in foals. A total of 105 diarrheic foals (n = 35 horse foals, n = 35 donkey foals, and n = 35 mule foals) were selected regardless of their etiologies. Additionally, 12 healthy foals (n = 4 horse foals, n = 4 donkey foals, and n = 4 mule foals) as control animals were selected for this study. Packed cell volume (PCV) an...
Muhammad Amir Iqbal1, Nabila Roohi1*, Ahmad Qureshy2
...re used to determine the serum levels of FT4, FT3, TSH, Creatinine, AST and ALT. Prominent reduction (P<0.001) of creatinine was observed in overt hyperthyroid state, when compared with controls. However, significant (P<0.05) improvement was noticed in it in post treatment group. Pronounced elevation (P<0.001) of ALT was observed in overt state when compared with controls. Subclinical subjects also manifested...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...higher anti-SS antibody, serum growth hormone and IGF-1 concentrations and average body weight than those in the control group (P < 0.05). Immunisation with ptCS/2SS-asd promoted the growth of rams similar to the effect of the simple co-injection of CpG and SS DNA vaccine. Thus, the fusion type of CpG adjuvant is an effective, simple, and low-cost method in enhancing the effect of SS DNA vaccine and the growth of ram lambs.
...
Tamoor Azeem1,*, M. Yasin Tipu1, Asim Aslam1, Sajjad Ahmed2, Salman Ahmed Abid1, Abdullah Iqbal3, Naeem Akhtar4, Muhammad Saleem4, Aamerzish Mushtaq4 and Sajid Umar4
...lues. On the other hand, serum analysis revealed increase in ALT, AST, ALP and GGT while decrease in total protein and albumin (p>0.05).
...
Muhammad Huzaifa Mehmood1, Muhammad Ahmad Iqbal2, Muhammad Daood1, Muhammad Rizwan Tariq3*, Khubaib Ali3
...eat while improvement in serum lipid profile was observed by 4% supplementation of oat seeds. It was also observed that fat percentage in meat was also reduced by oat seed supplementation.
...
Zhen-li Li1,2, Hui-fang Zhou2,*, Bo-ru Zhou2, Bei Liu2, Xiao-fei Jiang2 and Jian-ya Xu2
... intervention. Then, the serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were detected by ELISA, FSHβ and LHβ expression levels in the pituitary were determined by qPCR, and GnRHR expression levels were assessed by qPCR and western blot analysis. Simultaneously, transcription factors, such as c-Jun, Elk-1, Egr-1, Nur77, CREB, and transduction molecules in PKC-MAPK, Ca2+-CAM, and cAMP-PKA signaling pathways, such as PKC, p...
Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1
...e expression of CXCL8 in serum and its mRNA in neutrophils of the patients with chronic renal failure (CRF). It was used to dynamically observe of Hemodialysis on the concentration of the CXCL8 in serum and the levels of CXCL8 mRNA in neutrophils were respectively measured by ELISA and PCR. The house keeping gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference, the ratio of lgcDNA/lgGAPDH ...
Wen Chao Liu1,2, Kwan Sik Yun3 and In Ho Kim2,*
...n tended to increase the serum HDL cholesterol levels (quadratic, P=0.074). Furthermore, the relative weight of bursa of Fabricius was increased by supplementation with 1,3-DAG (quadratic, P<0.05). In conclusion, dietary supplementation of different levels of 1,3-DAG in broilers diets could improve the growth performance, modify blood lipid profile and promote the development of bursa of Fabricius.
...
Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu
...ve or negative effect on serum total protein (TP), albumin (Alb), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) (P<0.05 or P<0.01). The g.247075G>A mutation was significantly association with these measured serum biochemical indexes except for LDL-C. Significant additive effects were detected for all detected
Osman Ergene1,*, Isfendiyar Darbaz1, Serkan Sayiner2 and Selim Aslan1
...In the PAG-Milk and PSPB-serum tests, there was a difference (P <0.05) in terms of blood values between the 32nd day and the 40th day when EM was detected. Statistically significant differences in PAG-Serum test (P <0.001) and PSPB test (P <0.01) was obtained between animals that were pregnant on the 40th day and animals that had EM on the 40th day according to TRUS observations. Sensitivity and specifici...
Yang Liu1, Jing-xin Mao2, Xiao-dong Wei2, Man Yi2, Xiao-long Zhang2, Ke Zheng3, Xian-xin Chen4, Guo-Ze Wang5 and Bing-bo Chen1,*
...ad significant increased serum levels of IgA, IgG, IgM and IL-2 compared with the control group (P<0.05); the Lactobacillus BFA group had obviously increased levels of IgA, IgG and IL-2 than the control group (P<0.05). It proves that the BFA has a positive effect on improving the health indicators and nutritional status, blood physiology and biochemistry, weight gain of rats, which can effectively promote the growth of animals and raise feed reward. It r...
Iqra Bano1,*, Moolchand Malhi2, Pershotam Khatri3, Saeed Ahmed Soomro2, Hira Sajjad4, Ambreen Leghari5, Muhammad Awais2, Safia Kandhro6 and Shakeel Ahmed Lakho5 and Munaza Soomro7
...rent in both groups. The serum glutathione peroxidase (GPx) activity as well the testicular GPx activity was significantly increased in SY as compared to C. Furthermore, the serum T-SOD activity was significantly elevated in SY as compared to C. Moreover, the T-SOD activity in testicular tissues was also significantly increased in SY as compared to C. The results revealed that the SY supplementation has positive effects on g...
Muhammad Zain Saleem1,*, Raheela Akhtar1, Asim Aslam1, Muhammad Imran Rashid2, Zafar Iqbal Chaudhry1, Muhammad Adeel Manzoor1, Bilal Ahmed Shah3, Rais Ahmed4 and Muhammad Yasin5
...n this study 960 and 471 serum samples of goats and sheep were collected, respectively. After screening with Rose Bengal test (RBT), all seropositive samples were subjected to real-time PCR assay. RBT confirmed the seroprevalences of 19.32% ± 0.289 and 12.29% ± 0.0105 brucellosis in sheep and goats, respectively and real-time PCR confirmed the B. abortus in 74 samples (62.71% ± 0.044) out of 118 seropositive samples in goats while 6...
Fazal Abbas1, Muhammad Ijaz1,*, Zunaira Akhtar1, Khalid Mehmood2, Muhammad Zeeshan Hyder3 and Umair Iqbal4
...ificant correlation with serum electrolytes and trace elements....
Fan Yi1,2, Xiaobin Yang1, Shigen Ye1, Hua Li1 and Ruijun Li1,*
...pheral leukocytes count, serum lysozyme activity, phagocytic rate, phagocytic index, respiratory burst activity of head kidney cells, compared with the basal feed group (P<0.05 or P<0.01). The challenge test results also showed that the 0.5% and 1% HCT feeding had higher immune protection against Edwarsiella tarda infection; and the relative protection ratios were 60% and 50%, respectively. It was also obtained that interval feeding ...
Yuyu Wang, Gangchun Xu*, Zhijuan Nie, Quanjie Li, Nailin Shao and Pao Xu*
...s on growth performance, serum biochemical parameters, digestive enzymes activity and antioxidant status of largemouth bass (Micropterus salmoides) reared in an in-pond raceway system (IPRS). Fish (initial average body weight: 35.68±2.12g) were randomly allotted to in-pond raceways (26.2m×5m×2.5m) stocked at two stocking densities (68 and 114 fish/m3, respectively). Fish were fed twice daily (08:00 and 17:00), and the daily...
Hong Zhang1, Shu-Fen Han2, Jing Wang1, Shao-Kang Wang1, Gui-Ju Sun1 and Cheng-Kai Zhai1, *
... increasing, increase in serum triacylglycerol, total cholesterol, glucose and the decrease in high density lipoprotein cholesterol. However, CWD can improve blood lipid and blood sugar levels, and at the same time improve obesity and fat accumulation in rats. CWD significantly augmented the relative level of peroxisome proliferators-activated receptor γ (PPARγ) and suppressed the sterol regulatory element-binding protein 1c (SREBP-1c...
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
... in T2 and C groups, and serum PRL levels in T1 group was significantly higher than that in control group. Body weight of offsping rats in T1 group were significantly higher than that in control group at weeks 2 and 4 after birth, and body weight of offsping in T2 group were significantly higher than that in control group only at week 4. Oral SS-14 DNA vaccine fused tPA signal peptide and CpG adjuvant and delivered by attenuated Salmone...
Shaikh Abdul Lateef, Zhou Lin, Wu Jian, Junjie Qian, Jonathan Hartanto Tan and Zheng Shusen*
...fied into infectious and serum hepatitis. The pattern of disease progression manifests liver inflammation, chronic hepatitis and hepatocellular carcinoma. In this article, we present a comprehensive review characterizing the viral architecture, life cycle, infectivity mechanisms, immune escape strategies, disease epidemiology and preventive measures associated with HBV. The current antiviral therapeutics are not providing adequate protection against HBV. It is...
Shujjah Haider1,*, Ayesha Maqbool1, Tariq Pervez1, Saima Parveen1, Arfan Ahmad2, Zahid Iqbal3, Javed Iqbal3, Shahid Mehmood3, Amanullah Khan4 and Sajid Umar5
...tion of MG antibodies in serum samples as compared to RPA/SPA test. This evidence emphasizes the need of more systemic approaches for the investigation ofMG distribution and prevalence in otherparts of Pakistan in order to design effective control strategies.
...
Qudrat Ullah1,8,*, Huma Jamil1, Laeeq Akbar Lodhi1, Zafar Iqbal Qureshi1, Shakeeb Ullah1, Tariq Jamil2, Iahtasham Khan3, Shahbaz Bashir4, Qudratullah5, Inamullah Wazir6, M. Abdus Sallam1 and Muhammad Zubair7
...nfection. A total of 384 serum samples were collected from cross bred dairy cattle at three different private farms located in Gujranwala, Pakistan. The samples were analysed by Rose Bengal Plate Test (RBPT) and by indirect-Enzyme Linked Immunosorbent Assay (iELISA). The overall seroprevalence was found to be 28.90 and 27.86% by RBPT and iELISA, respectively. Previous history of animal reproductive disorders was found to be significantly associated (P=0.005...
Ali Mujtab Shah1,2,3, Muhammad Naeem2, Muhammad Giasuddin Shah2, Muhammad Haaroon2, Quanhui Peng1 and Zhisheng Wang1,*
...is study showed that the serum IgG levels and body weight gain were significantly (p < 0.05) different among the groups, the greater levels were observed in ST (15.74±0.29 mg/ml) as compared to NF (11.52±0.72 mg/ml) and NS (10.36±0.36 mg/ml) during the experiment period. Whereas; withers height, heart girth and body length were found non-significant (p > 0.05) among the groups. The morbidity rate of calves fed different levels of col...
Chukwuemeka Calistus Okolo1*, Ikenna Onyema Ezeh2, Chinelo Nnenna Uju3 and Nwakaego Ernestina Nweze1
...oups A and B had similar serum alanine and aspartate aminotransferases, blood urea nitrogen, and serum creatinine levels as group D. The serum malondialdehyde and catalase levels were similar in groups A--D. Therefore, treatment with the probiotics, while enhancing clearance of trypanosomes did not improve the antioxidative and clinical outcome of the infection. ...
Nouf Alharbi*, Mai Elobeid and Promy Virk
...MDA) levels in liver and serum, and histopathological evaluation of liver and kidney. Results showed that low dose of quercetin was significantly (p≤0.05) more effective than the high dose. Low dose was more efficacious in reducing Cd accumulation in the tissues, reversing the effects of Cd toxicity on blood profile, and on the CAT and SOD activity in the liver and decreased the MDA levels in both serum and liver. Thus, t...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...ein bound with the human serum almost three times more efficiently than the positive control. For raising antibodies against the antigenic protein HCV3a E2, the purified protein E2 was injected subcutaneously into the rabbit (Oryctolagus cuniculus). The antibodies titer in rabbit after immunization in 100% pure serum sample was 0.736 which was 15 times more than that of pre immune control sera (0.049). It is co...
Yue Ren1, Peng-Fei Liu2, Wan-Long Zhu1,*,Hao Zhang1 and Jin-Hong Cai1
...esis (NST), food intake, serum leptin levels and hypothalamic neuropeptide Y (NPY), agouti aelated peptide (AgRP), pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) expressions were measured. The results showed that body mass and serum leptin levels were lower significantly in winter than that of in summer in five areas. But thermogenic characteristics and food intake were higher significantly ...
Shad Mahfuz1,2, Shuyuan Wang1, Mo Chen1, Fei Zao1, Dong Zhen1,
Zhongjun Liu4 and Hui Song1,3*
...ormance, egg quality and serum metabolic profile during the early phase of production.A total of 105 ISA Brown 18 wk old laying hens were grouped into 5 treatments with 7 replications of 3 hens each. Dietary treatments included a basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW for 8 wk. Data were subjected to one-way analysis of variance using SPSS software followed by Duncan’s test probability of p<0.05. During...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...ntration of the CXCL8 in serum and the level of CXCL8 mRNA in PBMCs of forty-eight children were dynamically measured by ELISA and PCR. The ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in PBMCs in MPP children were (298.917±51.860) pg/mL and (1.848±0.525) lgcDNA/lgGAPDH. There were significant differences between the trial groups ...
Mo Chen1, Shad Mahfuz1, 2, Yan Cui1, Liying Jia1, Zhongjun Liu3 and Hui Song1, 4*
...ntioxidant properties of serum and egg yolk in ISA Brown layer hens fed with mushroom (Flammulina velutipes) stembase (FVS). A total 150 hens of 30 wk old were grouped into 5 equal treatments with 5 replications of 6 hens each. Dietary treatments included basal diet as a control group; control diet including antibiotic (0.05% flavomycin) as an antibiotic group; 2% FVS fed group; 4% FVS fed group and 6%FVS fed group. The experimental duration was total 63 days,...
Muhammad Furqan Shahid1*, Tahir Yaqub1, Muhammad Yasin Tipu2, Asim Aslam2, Saima Yaqub1, Aziz-ul-Rahman3, Muzaffar Ali1
... Cross reactivity of antiserum response toH9N2 was compared among reference isolates when challenged in broilers, Partridge origin anti-H9N2 antibodies showed varied hemagglutination inhibition results (4log2 to 15log2) when cross reacted with different antigens, suggesting H9N2 viruses are circulating with different antigenic characteristics. Sero-biochemical analysis of blood collected at 7th DPI revealed an increase in aspar...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...5 ml Blood was drawn and serum was separated. Anti-Oxidant Biomarkers, Serum micronutrients, serum Electrolyte balance was measured through spectrophotometric procedure. Serum MDA level jump high in patients (14.19) as compared to normal subjects (3.26). Serum Glutathione declined in disease persons (0.56) from healthy...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...ence to udder health and serum parameters of lactating goats. Thirty two lactating Beetal goats of 3-4 y age, weighing 40.91±0.285, were randomly selected, and equally divided into four groups. Group A was kept as control while animals of groups B, C, and D were individually fed daily with 30µg, 40µg and 50µg of AFB1, respectively, through naturally contaminated cotton seed cake for a 10 days period. Milk samples were tested for aflato...
Saman Muhsin Abdulkareem* and Nadir Mustafa Nanakali
...ve stress biomarkers and serum liver enzymes (p<0.05). While, pre and post treatment of QCT (20 mg/kg/day) had protective effects on hepatotoxicity induced by TCDD and could significantly decrease these factors (p<0.05). Furthermore, there is no difference between the pre and post treatment of QCT (p>0.05). In addition to, the results showed that prolonged exposure to TCDD causes mutation in CYP1A1 gene and increases the expression of this gene...
Zekeriya Demir1 and Hatice Kaya2*
...ncreased Mg value in the serum parameters. However, the other serum parameters were not affected by the treatment. In conclusion, supplementation of bee pollen during the study period improved the feed conversion ratio and decreased serum cholesterol and lipid contents. In addition, according to economic analysis, the net income for hen fed 1.5% BP addition dietincreased by about 0.62% com...
Hu Liu1,2, Qifang Yu1,2, Xiaopeng Tang1,2, Chengkun Fang1,2, Sijia Chen1,2 and Rejun Fang1,2,*
...oncentration in eggs and serum antioxidant capacity in laying hens. Seven-hundred and twenty 21-week-old healthy Roman laying hens with a similar laying rate were randomly divided into 5 groups with 6 replicates of 24 hens. The experiment was designed by 2 × 2 factorial arrangement with two sources of Se and two levels of Se. The hens in control group were fed a basal diet without adding exogenous selenium source, and the hens in other four groups were f...
Kadry A. El-Bakry1, Lamiaa E.M. Deef1*, Lotfy Z. Habbak1 and Samia S. El-Naeli2
... significantly increases serum level of malondialdehyde (MDA) but decrease the activity of superoxide dismutase (SOD) enzyme. On the other hand, in patuilin injected rats and treated with ginger, the activity of ALT, AST and SOD were improved and level of MDA was decreased significantly. The liver and kidney tissues showed markers of improvement after treatment the patulin rats with ginger. In conclusion, treatment with ginger can protect liver and kidney form...
Sameh S. Tawfik, Ahmed A. Elkady* and Ehab T. Mohamed
...limited the elevation in serum tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels when compared to irradiated rat group. The histopathological-findings of renal tissues in the irradiated-group, showed nephrotoxicity; the renal cortex showed massive necrotic changes, the convoluted tubules showed distinctive pattern of ischemic renal injury and the cuboidal epithelium cells of proximal and distal renal ...
Monica Paula Marin1, Elena Narcisa Pogurschi1*, Iuliana Marin2 and Carmen Georgeta Nicolae1
...ficant increase in blood serum calcium during the last week of precalving period, as well as in the calving and postcalving periods, having as consequence fewer recorded milk fever cases. The results obtained can convince the farmers to use volcanic tuff as a food additive in the feeding of dairy cows during the precalving, postcalving and lactation periods, in parallel with ensuring the selenium requirements, because the natural zeolite can improve the reprod...
Doulat Khan1, Hamayun Khan1, Nazir Ahmad2, Muhammad Tarique Tunio3, Muhammad Tahir2, Muhammad Saleem Khan2 and Rifat Ullah Khan1*
Yan-Guo Han, Yu-Qing Han, Jia-Yuan Wu, Jia Guo, Ri-Su Na, Yan Zeng, Guang-Xin E, Yong-Ju Zhao and Yong-Fu Huang*
...e in maternal animals on serum related hormones in offspring. In this study, ten ewes with two newborn lambs were randomly divided into SS DNA vaccine group (Group T) and empty vector group (Group C), and these vaccines were given orally into the ewes at weeks 0, 3 and 6 of the research. Blood samples of ewes or offspring lambs were collected at week 6 or 9 of lactation, respectively. Serum SS, prolactin (PRL), growth hormon...
Abdüssamet Aydın1 and Ĺž. Canan Bölükbaşı2*
...ion peroxidas (GSHPx) in serum. Sixty Lohman LSL white layers, 40 weeks of age, kept in individual cages were assigned randomly to five treatment groups, each group included 12 hens. The hens received one of five diets with 0, 32.5, 65 or 130 mg/kg pennyroyal extract and 50 mg/kg BHA, respectively. Experiment lasted for 60 days. At the end of the experiment, the supplementation of pennyroyal extract did not affect feed intake, rates of albumen, yolk and shell ...
Mingsan Miao*, Hui Zhao, Jiaojiao Jia, Xiaoyan Fang and Yanyan Miao
...mical indicators such as serum NSE in the brain tissue. To investigate the impact of total phenolic acid on the biochemical parameters in the repeated cerebral ischemia-reperfusion mice, the mouse model of cerebral ischemia repeated reperfusion was successfully replicated. The doses of total phenolic acid of D. canes have significantly reduced the levels of IL-6 and TNF-α in brain tissue and decreased the level of NSE in the seru...
Abdul Kabir¹, Amjad Hussian Mirani¹, Jam Kashif1, Shumaila Manzoor2, Abdullah Iqbal3*, Ihsan Ullah Khan4 and Muhammad Abubakar2
...era, KPK. A total of 246 serum samples were randomly collected from three age groups (<1 year, 1-2 year and >2 years) of sheep and goats. Competitive ELISA was performed to evaluate sero-prevalence of PPR in goats and sheep. The swab and tissue samples were collected from the surrounding areas from suspected sheep and goats to confirm the PPRV. Out of total 246 sera, 144 (58.54%) samples were found seropositive for PPRV. PPR was more prevalent in sheep (...
Fatima Hashim Abbas1,2* and Alaa Tareq Shakir Al-Hassnawi1
...OS) were measured in the serum of all studied groups. MDA levels were significantly higher (p≤0.05) in aborted women with T. gondii compared to negative control. MDA levels were also significantly higher (p≤0.05) among aborted women with Cytomegalo virus compared to negative and positive control groups. All aborted women had significantly higher levels of TAC levels (p≤0.05) compared to the control groups. However, catalase activity and ROS lev...
Ahsan Numan1,4, Khadija Irfan Khawaja2, Abdul Basit Qureshi3, Muhammad Shahbaz Yousaf4, Imtiaz Rabbani4, Hafsa Zaneb5 and Habib Rehman4*
...determination of various serum biochemical determinants. Prevalence of DSP was found to be 31.48% when 2,290 diabetes mellitus Type-2 subjects were screened. The patients with DSP were older (p < 0.05), had longer duration of DMT2 (p<0.01), elevated systolic blood pressure (p<0.05), blood sugar (p < 0.05), HbA1c (p<0.001), cholesterol (p<0.01), LDL (p<0.001), triglycerides (p<0.05) and more obese (p<0.01) when compared wit...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...me, creatinine, and both serum and urinary electrolyte levels were measured. In addition, the apoptosis rate of white blood cells was analysed from plasma samples. Tissue samples from the brain, heart, aorta and kidneys were used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF group and the control group with regard to heart rate,...
Jiao Qin,Qian-Qian Su and Quan-Sheng Liu*
Saima Sharif*, Farkhanda Manzoor, Tasnim Farasat, Shaugfta Naz and Raheela Tabasum
...stimation was done using serum creatinine and blood urea along with eGFR calculation by MDRD equation. Kidney dysfunction was defined as eGFR of <60 ml/min/1.73m2. Statistical analysis was donedone by using SPSS. The serum creatinine of ischemic stroke patients was found to be 2.41 mg/dl compared to 0.93mg/dl in controls. The blood urea in ischemic stroke group was 67.3 mg/dl in contrast to 34.6 mg/dl in controls. The eGF...
Gong Ga1,2 and SuoLang Sizhu2,*
... In this study, 173 serum samples of Tibetan swine were tested for anti-HEV IgM and IgG antibodies by the ELISA. HEV RNA were measured in feces (n=173), serum (n=173) and tissue ((including liver, spleen, kidney and intestine, n=21) by nested RT-PCR and qRT-PCR. HEV antigens were detected in tissues by immunohistochemistry analysis. Overall, we found that that eight serum samples (4.6...
Xuhui Zhang1, Zhiyuan Sun2, Jinfeng Cai1, Guibin Wang1, Zunling Zhu1, Linguo Zhao3 and Fuliang Cao1,*
... the FR3 diet. Moreover, serum glutathione (GSH) and α-tocopherol (α-TOH) contents in the FR groups were increased, while, levels of total cholesterol (TC), malondialdehyde (MDA) in serum and hepatic reactive oxygen species (ROS) in FR groups were significantly decreased compared with the control or NF group at 42 d of age. Furthermore, serum total superoxide dismutase (T-...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
...y included 500 suspected serum samples. All clinical specimens were tested for IgM and IgG specific antibodies against Chikungunya virus using a commercial ELISA kit. Twenty seven (5.4%) and thirty five (7%) samples were IgM and IgG positive, respectively. All antibody positive samples and a subset of negative samples (195) were further subjected to PCR for confirmatory purposes. Two different sets of primers were used for the identification of the viral genom...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
...(WB) with artificial antiserum. A total of 105 Haemaphysalis longicornis (H. longicornis) ticks were selected to prepare protein samples. Some of them were used for 2-DE, and the others were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protein spots representing 12 ORFs: 4 from ticks and 8 f...
Shaista Abbas1,Imtiaz Rabbani1, Hafsa Zaneb2, M. Shahbaz Yousaf1, Saima Ashraf2, Abid Hussain Shahzad3, M. Afzal Rashid4 and Habib Rehman1,*
...S), body weight (BW) and serum health biomarkers in Beetal goats during the transition period. Twenty-four Beetal goats were randomly allotted to three groups YSC0, YSC5 and YSC10 and were fed a basal diet supplemented with 0g, 5g or 10g yeast day/animal, respectively for 4 weeks before and -4 weeks after kidding. When compared with the non-supplemented goats, dietary yeast improved (P<0.05) DMI during transition period however, BCS (P = 0.81) and BW (P = 0...
H. Liu1, X.P. Tang1, R.J. Fang1,*, F.Yi2, C. Zhang2, R.Q. Yang1, F. Sun1 and S.Y. Zhou1
...n growth performance and serum Cu-Zn SOD activity in piglets. A total of 288 (144 castrated males and 144 females) healthy post-weaned at 23-day-old piglets (Duroc × Landrace × Yorkshire) with an average body weight of (8.79±1.15) kg were randomly divided into 6 groups with 8 replicates in each group, and 6 piglets (3 castrated males and 3 females) per replicate. Control group was fed a basal diet, experimental groups were fed diets suppleme...
Khayyam1*, Muhammad Zahid1, Naiz Ali2 and Qadeem Khan3
... patients. Prolactin and serum gonadotropin showed fluctuations in Hashimoto’s thyroiditis patients but in Grave’s disease patients only the gonadotropins showed abnormal values while prolactin was in the normal range. The study suggests that autoimmune thyroid diseases effect the pituitary-hypothalamic axis which needs to be further investigated to find the mechanism underlying it.
...
Mian Adnan Kakakhel1, Faryal Gohar1, Zahid Anwar2, Raza Ullah1, Muhammad Attaullah3, Shahbaz Ahmad4, Aamir Khan5, Kalimullah5*
... Peshawar, Pakistan. The serum samples were analyzed for IgG and IgM by using enzyme-linked immunosorbent assay (ELISA), and statistical analysis carried out. Out of these tested samples, 20% were positive for both IgG and IgM against T. gondii. The positive prevalence of T. gondii was 11.11% in male dogs and 42.85% in female dogs. Further details of results are given in below in main data. T...
Majeeda Rasheed1*, Tanveer Akhtar1, Nadia Mukhtar2, Muhammad Furqan Shahid2, Muhammad Imran3, Saima Yaqub2
Magbolah Salem Helal Alzahrani
...demonstrated decrease in serum lipoprotein HDL, LDL, VLDL fraction levels, indicating liver damage. Rats given CCl4 and fed on 5% M. chamomilla showed the most significant increase in organ weight as compared to all levels of treatment suggesting that M. chamomilla can reduce liver damage. Rats given CCl4 then fed on a combination of all levels of M. chamomilla showed a decrease of AST, ALT and ALP enzyme levels in th...
Mumtaz Ali Khan1, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Kashif Prince1*, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4, Asad Ullah5, Naimat Ullah4, Muhammad Shoaib2 and Said Sajjad Ali Shah2
...ets, packet cell volume, serum creatinine, bilirubin, liver enzymes, total, glucose and urea in blood significantly (P<0.05) increased. Fluctuations in mean erythrocyte counts (RBC), hemoglobin, PCV and total bilirubin were beyond the limits while others were within normal ranges in sheep infected with Clostridium perfringens type A. The total bilirubin, Liver enzymes, creatinine level, glucose and urea levels in blood were abnormal while others were...
Muhammad Shakeel1*, Mudussar Nawaz1, Zahid Naseer1, Muhammad Fiaz1, Asghar Khan1, Muhammad Imran Khan1, Awais Ur Rehman1, Ahmad Yar Qamar2 and Ali Raza3
Caprine and Ovine Serological Evidence of Brucellosis in Five Districts of Punjab, Pakistan
...akistan. A total of 1239 serum samples were collected from male (n=73) and female (n=1166) different sheep (n = 865; Pak-Karakal, Thalli, Lohi, Kajli and non-descript type) and goat (n = 374; Teddy, Beetal and non-descript type) breeds of variable age groups (0 months to 4years). All the serum samples were analyzed using Rose Bengal Plate Test (RBPT) and seropositive samples were further submitted to
Amit Sharma1* and Pankaj Sood2
Sonographic Assessment of Ovarian Follicular Dynamics during Breeding and Non-Breeding Season in Gaddi Goats
...ing both the seasons for serum progesterone (P4) estimations. Follicular growth was characterized by presence of three to five numbers of follicular waves in both seasons. Significantly higher (P<0.01) number of follicular waves (4.0±0.21 versus 3.18±0.12), number of follicles at wave emergence during second (2.85±0.26 versus 1.77±0.14; P<0.01) and third wave (2.57±0.2 versus 2.0±0.16; P<0.05), along with shor...
Zahir Shah1*, Saima Munawar1, Ihsan Ullah2, Taif Shah3, Haq Aman Ullah1, Saqib Nawaz1, Farhan Anwar Khan1 and Manoj Kumar Shah4
Combined Physico-chemical and Analgesic Effects of Electroacupuncture Plus Clonidine in Goats
...r, a significantly lower serum glucose concentration was noted in EA plus 10µg/kg of clonidine treated goats at 15-60 min than those treated with clonidine alone. Creatinine, blood urea nitrogen, alanine/aspartate aminotransferase activities, and hematological variables for differently treated groups were insignificant. In conclusion, EA combined with a low dose of 10µg/kg of clonidine provided better analgesia in experimental animals....
Muhammad Tahir Khan1, Shahid Mehmood2*, Athar Mahmud2, Khalid Javed3 and Jibran Hussain2
...fferences (P>0.05) in serum biochemical indices, and immune-related parameters among the diets. The experimental group fed compost at 10% showed the lowest (P=0.0001) feed cost per kg weight gain compared to control group. These results indicate that it is possible to feed diets containing up to 10% compost to growing meat quails without compromising growth performance, carcass characteristics, serum biochemical indices, ...
Joseph Anejo-Okopi1*, Dorcas Yilger Gotom1, Noble Allison Chiehiura1, Julius Ocheme Okojokwu1, David Ochola Amanyi2, John Otumala Egbere1, Joshua Adetunji1, Otobo Innocent Ujah3 and Onyemocho Audu4
The Seroprevalence of Zika Virus Infection among HIV Positive and HIV Negative Pregnant Women in Jos, Nigeria
...us IgG (ZV-IgG) in human serum. A total of 90 pregnant women was recruited, and the overall seroprevalence was (14.4%) for Zika virus (20.0% HIV positive and 8.9% negative) IgG antibodies. A significant association was found with age category among the HIV negative pregnant women p=0.012, and in the history of blood transfusion among the HIV positive participants, p=0.013. No association with educational level, employment, marital and resident, gestational tri...
Rida Younas1*, Tamseela Mumtaz1, Nabeela Roohi2 and Muhammad Amir Iqbal3
...sion of IL-6 and IL-8 in serum samples were evaluated by Enzyme-linked immunosorbent assay. Correlation between serum inflammatory cytokines (IL-6 and IL-8) level and post predicted FEV1%, FVC% and FEV1/FVC was analyzed. Results of serum IL-6 and IL-8 showed significant difference among control and GOLD stages II, III and IV (both P< 0.05). The level of IL-6 was highest in GOLD stage II...
Yasir Sharif1, Saba Irshad1*, Ammara Muazzam1, Muhammad Hamza Tariq1, Ambreen Kanwal1, Sana Rasheed1, Mabel Baxter Dalrymple2 and Anam Tariq1
...ount (CBC), blood group, serum ferritin level and liver function tests were performed. Secondary complications were assessed by physical examination of pallor, splenomegaly, ascites, and hepatomegaly. The average±SD values of patients’ CBCs and liver enzymes were: red blood cells 3.07x 1012±0.769x 1012/L, white blood cells 8.89x 109±2.849x 109/L, hemoglobin 8.01± 1.027 g/dL, platelet...
Mehwish Saleem Khan
Evaluation of Changes in Zinc Levels in Patients Suffering from Cancer
...this study zinc level in serum of cancer patients was observed. For this purpose, the blood of 50 cancer patients was taken from MAYO HOSPITAL Lahore. The patients are suffering from different type of cancers such as, breast cancer, lung cancer, uterus cancer, oral cavity cancer, esophageal cancer, urinary bladder cancer, blood cancer and brain tumor. In addition to serum zinc level of cancer patients many other parameters w...
Md. Gulam Rabbany Rassel1, Kazi Afsana Homayra Orchy1, Md. Moshiur Rahman Khan1, Marzia Rahman2 and Md. Mahmudul Alam1*
Hematological and Serum Biochemical Indices in Calves with Navel Ill
...es in haematological and serum biochemical attributes in calves affected with navel ill. Peripheral blood was collected from affected and healthy calves, and serum was separated for biochemical evaluation. In term of haematological profile, Hb and TEC were decreased whereas PCV and TLC were elevated compared to healthy calves. Some leading enzymes such as ALT, AST, ALP, LDH, CK were elevated in affected calves than their hea...
Jin-Juan Wan, Mei-Fang Shen*, Hui Xue, Hong-Yan Liu, Mei-Qin Zhang, Xi-He Zhu and Chong-Hua Wang
...i><0.05). The highest serum lysozyme (LZM), lowest catalase (CAT) and malondialdehyde (MDA) were observed in crayfish fed 3 times/day. However, there were no significant differences in cortisol, glucose (Glu), and superoxide dismutase (SOD) among all groups (P>0.05). The cumulative mortality in F3 was significantly lower than that of the other groups at d4 after challenge (P<0.05). In conclusion, both low and high feeding frequencies cou...
Xuya Zhou1, Ying Liu1, Deqin Xu1, Jie Bao1, Yaru Cao2 and Yong Jin3*
...c peptide (BNP) in blood serum of diabetes complicating arthritis rats. Blood pressure was measured by a noninvasive caudal artery blood pressure meter. Western blot analysis showed that unification of two drugs caused protein expression levels of COX-2 to reduce obviously and increased protein expression levels of CYP4A1. Immunohistochemistry showed the increased expression of CYP4A1. Elisa indicated that the decreased production of PGE-2 and the increased pr...
Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu*
...evels in the asthma mice serum. Inflammatory cells in the mice BALF are significantly reduced in a dose-dependent manner through quercetin. Quercetin (P<0.05) significantly reduced the level of TNF-α, IL-1β, IL-4 and IL-5 cytokines and increased the level of IFN-π in allergic asthma mice caused by OVA. In contrast, there was no significant effect in the body weight of mice treated with quercetin. Based on the result, we may infer that querceti...
Muhammad Shafiq1,2,*,Rajwali Khan1, Ilyas Ali3, Sadeeq Ur Rahman4, Saif Ullah3, Shah Jan Mohammad2, Mohammad Jan2 and Jinhu Huang2
...le breed type on (a) cow serum IgG and serum total protein concentration (b) colostrum immunoglobulin level and (c) their respective calves’ serum immunoglobulin and serum total protein concentration. Three breeds of cattle were observed: Jersey, Holstein Friesian (HF) and local Pakistani cow breed Achai. To assess s...
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
...ty of ATFs. Fetal bovine serum (FBS) is used extensively in cell culture but being expensive and scarce in availability, its alternatives are needed. This study proposed autologous equine serum (AES) and adult bovine serum (ABS) to be evaluated as alternative to FBS. ATFs were cultured in DMEM-HG and supplemented with 10% FBS, 10% AES and 10% ABS. At passage 4, each group showed same morph...
Rabia Riaz1, Khushi Muhammad1*, Masood Rabbani1, Muhammad Amjad Iqbal2, Aamir Riaz Khan2, Sadia Sarfaraz1, Mudassar Naseer3 and Khalid Majeed1
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
... medium (SSCM), which is serum-free. SSCs began to proliferate on the second day and quickly formed grape-like clusters that were consistent with the morphological features of SSCs. These cells were identified as positive by immunofluorescence staining. This study successfully isolated, enriched and identified buffalo SSCs and established an effective platform to explore the mechanisms of proliferation and differentiation of buffalo SSCs.
...
Kamal Al-Samawi1, Mohamed Al-Hassan2, Hussein Migdadi3,4*, Megahed Ammar5 and Salem Alghamdi3
...using real-time PCR, and serum progesterone (P4) concentrations were assayed using an ELISA kit. Pregnancy detection was performed by US on 23, 35, and 60 days post NM. Serum P4 concentration was significantly higher in pregnant than non-pregnant goats at 15, 23, 35, and 60 days post NM. Relative expression of mRNA of ISG15 and ISG17 was significantly higher in pregnant goats at 7, 15, and 35 days post N...
Ying Liu1, Shankun Liu1, Hui Wang2 and Weihua Su2,*
...The blood glucose level, serum, lipids, antioxidant, cytokines and C-peptide was recorded. GDM group rats showed the lower weight of pregnant rats, fetal and placental weight as compared to other group rats. Caffeic acid treated group rats exhibited the modulated level of blood glucose level, lipid profile and antioxidant parameters at dose dependent manner. Caffeic acid significantly (P<0.001) enhanced the level of insulin, hepatic glycogen as compared to ...
Nosheen Basharat1, Usman Waheed2,3*, Muhammad Arshad1, Noor e Saba4, Iram Masood1, Akhlaaq Wazeer1, Ahmad Farooq1, Sadaf Moneeba1, Abdul Rauf5 and Hasan Abbas Zaheer2,3
...iscovered in 1997 in the serum of a Japanese patient. TTV is a non-enveloped small virus of 3.8 kb containing a circular single-stranded negative-sense DNA genome. TTV represents the first circovirus-like virus found in humans. The study was conducted to assess the molecular epidemiology of TTV in healthy blood donors and find its relationship with hepatitis B and hepatitis C seropositivity. The study was conducted at the Department of Pathology and Transfusio...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
... using NDV reference antiserum, As well as realtime polymerase chain reaction by using standardized NDV specific primers and finally eight viruses were selected for further sequencing for the partial fusion protein, The eight NDVs isolates of velogenic genotype VII and contain the unique cleavage site motif 112RRQKRF117 with high relation to very virulent NDV Chinese strain Chicken /China/SDWF07/2011 strain with nucleotide identity percentage (99.3% -100%). Th...
Hagar E. Mohammed1, Ranwa A. Elrayess2 and Heba N. Gad El-Hak2*
...indices and a decline in serum testosterone levels which may have a bad impact on male infertility, on the other side, treatment the rats with the combined extract of different doses of A. monosperma or M. piperita attenuated the deleterious damage to the testes, the sperm index and the sex hormones. This study indicated that the traditional use of these two herbs as a mixture has no effects on male reproductive health.
...
Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*
...al signs, hematology and serum biochemistry in Nili Ravi buffaloes under subtropics is lacking. This study was designed to fulfill this gap by analyzing the effects of exposure to the bacterial culture of Pasturella multocida (P. multocida) type B:2 and its immunogens, i.e., lipopolysaccharide (LPS) and outer membrane protein (OMP) on Nili Ravi buffaloes. Prepubertal Nili Ravi female buffaloes (N=18) of 10-12 months age in good health condition were divided in...
Mehwish Saleem1*, Maria Tahir1, Maryam Ilyas2 and Aqsa Malik2
... purpose was to evaluate serum calcium level in patient’s blood. Blood samples were collected from cancer patients from MAYO hospital LHR during the time period April 2018 to May 2018. Some cancer patients have increased calcium level in blood. Cancer is more prevalent in males (n=17) 57% than females (n=13)43%. The most frequent cancer type among males is blood 41cancer (n=5) 36%, lung cancer (n=3) 22% and oral cancer (n=3)22%. In females br...
Aneeqa Saleem1, Muhammad Islam1, Hamid Saeed1* and Mehwish Iqtedar2
...utcome measures included serum creatinine, uric acid and BUN levels. Significant differences exist with regards to primary and secondary metabolites between both peel and pulp extractives. After 21 days of treatment in mice, only chloroform (CHL) extractives of peel and pulp demonstrated significant improvements in serum creatinine (Peel: N; 1±0.44, PC; 1.4±0.62, CHL; 0.43±0.18, p<0.05, ...
Xiujun Yao1, Haofeng Wang 2, Ligong Zhang3, Jingzhang Wu4 and Lijun Wang1*
... urine protein quantity, serum levels of IL-6, IFN-γ, and TNF-α ,kidney ROS and MDA levels, relative content of TGF-β1 and CTGF in TGP group were lower than that in model group (P <0.05); serum levels of IL-2 and CTLA-4,SOD levels in TGP group were higher than that in model group (P <0.05).These results indicated that TGP could reduce urinary protein quantity, inhibit inflammatory response, and reduce ...
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
.... Blood was collected in serum separating tubes through intracardiac puncture for biochemical analysis. Morphometric observations revealed significant decrease (P≤0.001) in body weight of mice in Al treated groups as compared to control but organs weight increased contrary to the body weight. Significant increase (P≤0.01-P≤0.001) was recorded in total alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) in liver and ure...
Saddar Faheem1,2, Hameeda Kalhoro1, Naeem Tariq Narejo1*, Muhammad Hanif Chandio3, Memon Samina4, Shahnaz Rashid5 and Ghulam Abbas5*
...tological variations and serum biochemical constituents of Labeo rohita from Kotri Barrage near Jamshoro, Sindh, Pakistan. Fish samples (100 specimens for each sex) were collected during two seasons i.e., summer: March to May, 2017 and winter: November, 2017 to January, 2018. Haematological characteristics of the fish specimens were considered season and sex wise separately. Results showed that erythrocytes (2.09 × 106 to 2.26 × 106), leucocytes (1...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
... parameters, hematology, serum biochemistry, immune response (ND and SRBC), intestinal histo-morphology, bacterial enumeration and cecal volatile fatty acids (VFAs) were measured. There were significant improvements in weight gain (WG), feed conversion ratio (FCR) and average daily gain (ADG) in the treatment groups supplemented with extract (P<0.05) compared to control. Similarly, intestinal histo-morphic parameters and ileal bacterial count were significa...
Ahmed F. Afify1, Mohamed A. Shalaby2, Ahmed A. El-Sanousi2 and Amal S. Gaber1
...se breeds, including 540 serum samples, among these, 4 EDTAblood
and 4 semen samples. Serologically, indirect ELISA was performed on 540
serum samples. 130 samples were clearly strong positive at a percentage of 24%.
Molecular detection of EAV genome was performed only on 8 selected semen and
EDTA-blood samples which were giving highly distinct positive reaction in the
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